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Thyroid hormone immunochromatographic test strip and preparation method thereof, and thyroid hormone immunochromatographic kit

A technology of immunochromatographic test strips and thyroid hormones, which is applied in the direction of analyzing materials, biological testing, material inspection products, etc., to achieve the effects of high sensitivity, improved detection sensitivity, and improved accuracy and sensitivity

Inactive Publication Date: 2020-03-10
海卫特(广州)医疗科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Based on this, it is necessary to provide a thyroid hormone immunochromatographic test strip and its preparation method and kit for the problem of limited detection of animal thyroid hormones

Method used

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  • Thyroid hormone immunochromatographic test strip and preparation method thereof, and thyroid hormone immunochromatographic kit
  • Thyroid hormone immunochromatographic test strip and preparation method thereof, and thyroid hormone immunochromatographic kit
  • Thyroid hormone immunochromatographic test strip and preparation method thereof, and thyroid hormone immunochromatographic kit

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preparation example Construction

[0043] In one embodiment, the present invention also provides a method for preparing any one of the above-mentioned thyroid hormone immunochromatographic test strips, comprising the following steps:

[0044] S1. Add the sample pad treatment solution to the sample pad and dry it.

[0045] The specific steps are: spray the sample pad treatment solution on the sample pad at a speed of 2-8 μL / cm, and dry it overnight.

[0046] In one embodiment, the sample pad treatment solution is a sample pad diluent containing 0.5-2 mg / mL mouse IgG, 0.5%-1% Evans blue and 0.1-2 mg / mL RBC, and the sample pad diluent is a sample pad diluent containing 0.5%- 3% Tween-20 and 2%-5% BSA (Bovine Serum Albumin) in 0.02M PB buffer (Phosphate Buffer).

[0047] S2. The fluorescent microsphere solution, animal thyroid hormone monoclonal antibody and the first complex are prepared into a labeling solution, and then the labeling solution is added to the labeling area of ​​the labeling pad and dried.

[0048]...

Embodiment 1

[0075] Preparation of detection membrane

[0076] Dilute animal thyroid hormone antigen to a concentration of 0.5mg / mL with 0.01M PB (pH=7.4) buffer solution containing 5% sucrose, which is the working solution of the detection area (T line);

[0077] Dilute the rabbit IgG antibody to a concentration of 0.5mg / mL with 0.01M PB (pH=7.4) buffer solution containing 5% sucrose, which is the working solution of the quality control area (C line);

[0078] Stick the nitrocellulose membrane on the PVC board, draw the detection area and the quality control area on the membrane, the concentration of the membrane is 1 μL / cm, and place it at 50°C to dry for 48h.

[0079] Preparation of sample pads

[0080] Spray the sample pad treatment solution on the glass fiber (sample pad) at a speed of 2 μL / cm, and dry it overnight; wherein, the sample pad treatment solution contains 0.5 mg / mL mouse IgG, 0.5% Evans blue and 0.1 mg / mL The sample pad diluent of RBC, the sample pad diluent is 0.02M PB ...

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Abstract

The invention relates to a thyroid hormone immunochromatographic test strip and a preparation method thereof, and a thyroid hormone immunochromatographic kit. The test strip comprises a substrate, anda sample pad, a marking pad, a detection film and an absorption pad which are arranged on the substrate, wherein the sample pad, the marking pad, the detection film and the absorption pad are sequentially connected from one end to the other end of the substrate; and the marking pad comprises a marking area, the marking area contains an animal thyroid hormone monoclonal antibody marked by fluorescent microspheres and a first complex marked by the fluorescent microspheres; the detection film is provided with a detection area and a quality control area, the detection area is coated with an animal thyroid hormone antigen, the quality control area is coated with a second complex, and the first complex and the second complex can be specifically combined. According to the thyroid hormone immunochromatographic test strip, fluorescence signals are detected by means of an immunoassay analyzer, the detection sensitivity is greatly improved by a photoelectromagnetic signal amplification system, interference of background colors is effectively eliminated, misjudgment of human factors on results is reduced, and the thyroid hormone immunochromatography test strip is safe, simple and rapid to operate and high in sensitivity.

Description

technical field [0001] The invention relates to the technical field of immunochromatography detection, in particular to a thyroid hormone immunochromatography test strip, a preparation method and a kit thereof. Background technique [0002] Thyroid hormone (T4) has very important physiological functions. It can promote the growth and development of animals, promote the basic metabolism of carbohydrates, proteins, and fats in animals, increase the heat production in animals, and act on the central nervous system, cardiovascular system, and digestive tract. and other organizations. Insufficient synthesis and secretion of thyroid hormones can lead to hypothyroidism (hypothyroidism), while excessive synthesis and secretion of thyroid hormones can lead to hyperthyroidism (hyperthyroidism). Therefore, detection of thyroid hormone (T4) in serum is of great significance for evaluating whether thyroid function is normal. [0003] At present, the immunological methods for detecting ...

Claims

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Application Information

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IPC IPC(8): G01N33/78G01N33/533G01N33/558
CPCG01N33/533G01N33/558G01N33/78
Inventor 杨晶邓艳珍黄晨珠梁才弗岑赞询王伟
Owner 海卫特(广州)医疗科技有限公司
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