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Method for extracting pigskin collagen through acid leaching
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A pig skin collagen and acid leaching technology, applied in the field of food processing, can solve the problems of low product purity, low collagen yield, corrosion of production equipment, etc., and achieve the effect of high quality and high extraction rate
Inactive Publication Date: 2020-03-17
FOSHAN UNIVERSITY
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Problems solved by technology
The acid extraction method has the advantages of rapid reaction and short processing time, but the collagen yield of this method is low, and the corrosion of production equipment is serious
In addition, when using strong acids such as hydrochloric acid for extraction, the time, temperature and pH of acid leaching are all important factors affecting the extraction effect of collagen. When these factors are not designed properly, collagen may be completely hydrolyzed into amino acids, or make Part of the amino acid structure (such as tryptophan, serine, etc.) is damaged, and the purity of the product is low due to the varying degrees of hydrolysis of the collagen produced
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Embodiment 1
[0023] A method for extracting pigskin collagen by acid leaching, comprising the following steps:
[0024] 1) After cleaning the fresh pigskin, use a scalpel to scrape off the subcutaneous fat and surface hair, and trim the pigskin into a 1cm×1cm square; wash it with phosphate of pH 7.5, then soak it in distilled water and rinse it for 4-5 times, drained, vacuum freeze-dried for 48 hours, pulverized, and stored at -4°C;
[0025] 2) Immerse the pigskin powder after the above treatment in a 10wt% NaCl solution for 12 hours according to a solid-liquid ratio of 1:50 (w / v) to remove salt-soluble impurities. After removing impurities, centrifuge at 8000r / min for 10min to obtain a solid precipitate;
[0026] 3) Mix the pretreated pigskin samples with acetic acid solutions of different pH (1.8, 2.1, 2.4, 2.7, 3.0), the ratio of solid to liquid is 1:50 (w / v), acid hydrolyze for 24 hours, and then 8000r / min after acid hydrolysisCentrifuge for 10min, take the supernatant, add NaCl for ...
Embodiment 2
[0033] A method for extracting pigskin collagen by acid leaching, comprising the following steps:
[0034] 1) After cleaning the fresh pigskin, use a scalpel to scrape off the subcutaneous fat and surface hair, and trim the pigskin into a 1cm×1cm square; wash it with phosphate of pH 7.5, then soak it in distilled water and rinse it for 4-5 times, drained, vacuum freeze-dried for 48 hours, pulverized, and stored at -4°C;
[0035] 2) Immerse the pigskin powder after the above treatment in a 10wt% NaCl solution for 12 hours according to a solid-liquid ratio of 1:50 (w / v) to remove salt-soluble impurities. After removing impurities, centrifuge at 8000r / min for 10min to obtain a solid precipitate;
[0036] 3) The pretreated pigskin samples were mixed with citric acid solutions of different pH (1.8, 2.1, 2.4, 2.7, 3.0), the ratio of solid to liquid was 1:50 (w / v), acidolysis for 24 hours, 8000r / v after acidolysis Min centrifuge for 10 min, take the supernatant, add NaCl for salting...
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Abstract
The invention belongs to the technical field of food processing, and discloses a method for extracting pigskin collagen through acid leaching. The method comprises the following steps: 1) cleaning pigskin with phosphate, washing with water, draining, freeze-drying and crushing the material to obtain pigskin powder; 2) soaking the pigskin powder in a sodiumchloride solution for 10-16h, and carrying out centrifugation to obtain a precipitate, and 3) adding the precipitate into an acid and carrying out acidolysis and performing subsequent treatment to obtain pigskin collagen; wherein the acid isone or more of acetic acid, citric acid and lactic acid. The method is simple, and the obtained collagen is high in extraction rate and good in quality.
Description
technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a method for extracting collagen from pigskin by acid leaching. Background technique [0002] With the rapid development of the national economy and the increasing level of people's consumption, my country has become a big producer and consumer of pork in the world, but a large amount of pig by-products (such as pigskin) will also be produced when pork is consumed in large quantities. Pig skin is the main by-product of pork slaughter, and its main application field is the leather industry. But because the leather quality that pigskin is made is inferior, and the utilization rate that pigskin is used for tanning is only 20~30%, so the utilization rate of pigskin as tanning material is decreasing year by year. However, pigskin is useful in addition to being used as a tanning material. The protein content in pigskin is as high as 33%, of which about 87.7% is co...
Claims
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Application Information
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