Cell model for screening developmental toxicity exogenous compounds by taking 11beta-HSD2 as target, as well as construction method and application of cell model

A cell model and compound technology, applied in the field of drug toxicology, can solve the problems of lack of mechanism theory system, difficult cultivation, poor specificity, etc., and achieve the effect of mature extraction and identification methods, simple growth environment, and strong binding specificity
CN110904047AActive Publication Date: 2020-03-24WUHAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WUHAN UNIV
Publication Date
2020-03-24

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Abstract

The invention provides a cell model for screening developmental toxicity exogenous compounds by taking 11beta-HSD2 as a target, as well as a construction method and application of the cell model. Human WJ-MSCs is adopted as target cells, luciferase reporter genes containing 11beta-HSD2 promoters are transfected into the target cells to obtain the cell model, and the model has two indexes: 11beta-HSD2 gene expression and luciferase activity of a reporter system. By means of the cell model, 11beta-HSD2 gene expression and luciferase activity of a tested compound treatment group are reduced, andit is prompted that the tested compound has developmental toxicity. The cell model for screening developmental toxicity exogenous compounds has the characteristics of high specificity, high sensitivity, high stability, high feasibility and the like, can be used for high-throughput screening, and has important significance for rapid screening of developmental toxicity exogenous compounds.
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Description

technical field

[0001] The invention belongs to the field of drug toxicology, and relates to a cell model for screening developmental toxic exogenous compounds with 11β-HSD2 as a target, a construction method and application thereof. Background technique

[0002] Developmental toxicity of exogenous compounds refers to the structural or functional damage caused by exogenous compounds in the offspring before sexual maturity, including structural deformities, dysfunction, growth retardation and even death. Developmental toxicity is one of the important contents of safety evaluation of exogenous compounds. Most of the current evaluation methods use the results of the overall animal reproduction experiment as a measure of the developmental toxicity of exogenous compounds, but there are disadvantages such as large amount of animals used, long experimental period, poor specificity, low sensitivity, and the inability to elucidate its mechanism from the cellular or molecular level. ...

Claims

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