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CAR-T living body tracing method

A CAR-T and in vivo technology, which is applied in the direction of pharmaceutical formulations, radioactive carriers, and in vivo radioactive preparations, can solve the problems of long radiation exposure time, long half-life of nuclides, and high radiation dose, and achieve short imaging time and half-life. Effect of short duration and low radiation dose

Active Publication Date: 2020-03-27
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Therefore, the technical problem to be solved in the present invention is to overcome the long half-life of the nuclide, the long time of radiation exposure to the living body, and the high radiation dose, which are easy to cause damage to the living body when the practical nuclide is used for CAR-T cell tracing in the prior art. Shortcomings

Method used

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Examples

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Embodiment 1

[0028] A CAR-T tracer method in vivo, comprising the following steps:

[0029] S1. Combining pre-targeting molecule A with amino groups on the surface of CAR-T cells to obtain pre-targeting CAR-T cells with A groups;

[0030]

[0031] where R 2 -NH 2 in, R 2 That is, it represents the main body of CAR-T cells, -NH 2 Represents the amino groups on the surface of CAR-T cells. The above reaction formula is the process of binding the pre-targeting molecule A to the amino group on the surface of the CAR-T cell membrane. The pre-targeting molecule A with an isothiocyanate group can bind to the amino group on the cell membrane surface under mild conditions to form a stable covalent bond , so that the pre-targeting molecules are firmly bound to the surface of the cell membrane. The advantage is that the pre-targeting molecules can be directly added to the conventional cell culture medium, and the amount of addition is less than micrograms. With the long-term proliferation and c...

Embodiment 2

[0042] A CAR-T tracer method in vivo, comprising the following steps:

[0043] S1. Combining pre-targeting molecule A with amino groups on the surface of CAR-T cells to obtain pre-targeting CAR-T cells with A groups;

[0044]

[0045] where R 2 -NH 2 in, R 2 That is, it represents the main body of CAR-T cells, -NH 2 Represents the amino groups on the surface of CAR-T cells. The above reaction formula is the process of binding the pre-targeting molecule A to the amino group on the surface of the CAR-T cell membrane. The pre-targeting molecule A with an isothiocyanate group can bind to the amino group on the cell membrane surface under mild conditions to form a stable covalent bond , so that the pre-targeting molecules are firmly bound to the surface of the cell membrane. The advantage is that the pre-targeting molecules can be directly added to the conventional cell culture medium, and the amount of addition is less than micrograms. With the long-term proliferation and c...

Embodiment 3

[0056] A CAR-T tracer method in vivo, comprising the following steps:

[0057] S1. Combining pre-targeting molecule A with amino groups on the surface of CAR-T cells to obtain pre-targeting CAR-T cells with A groups;

[0058]

[0059] where R 2 -NH 2 in, R 2 That is, it represents the main body of CAR-T cells, -NH 2 Represents the amino groups on the surface of CAR-T cells. The above reaction formula is the process of binding the pre-targeting molecule A to the amino group on the surface of the CAR-T cell membrane. The pre-targeting molecule A with an isothiocyanate group can bind to the amino group on the cell membrane surface under mild conditions to form a stable covalent bond , so that the pre-targeting molecules are firmly bound to the surface of the cell membrane. The advantage is that the pre-targeting molecules can be directly added to the conventional cell culture medium, and the amount of addition is less than micrograms. With the long-term proliferation and c...

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Abstract

The invention relates to the technical field of living body tracing, in particular to a CAR-T living body tracing method, and aims to solve the problem that in the prior art, radioactive nuclein enters living bodies (animals or human bodies) along with CAR-T cells to damage the CAR-T cells and the living bodies. The method is characterized by comprising the following steps of S1, combining pre-landed molecule A with amino on the surfaces of the CAR-T cells to obtain the pre-landed CAR-T cells having A groups, wherein R2-NH2 is the CAR-T cells, and -NH2 is the amino groups of the surfaces of the CAR-T cells; S2, combining 18F with homing molecules B, to obtain R1-N3 of 18F; S3, injecting the pre-landed CAR-T cells obtained in S1 into the living bodies, during required imaging time points, injecting the R1-N3 into the bodies, and combining the R1-N3 with the pre-landed CAR-T; and S4, through PET / CT scanning, observing the distribution condition of the CAR-T cells in the living bodies. According to the CAR-T living body tracing method, the operation safety of experimenters is improved, and damage of radioactivity to the CAR-T cells and the living bodies can be avoided.

Description

technical field [0001] The invention relates to the technical field of living body tracing, in particular to a CAR-T living body tracing method. Background technique [0002] In recent years, common malignant tumors of the blood system such as leukemia, myeloma, and lymphoma have made great progress through the application of chemotherapy and bone marrow transplantation technology, but there is still a high treatment failure rate, and the problems of drug resistance and recurrence are still difficult to overcome. Therefore, it is urgent to find another way and explore safer and more effective treatment methods. [0003] Chimeric antigen receptor T cell (chimeric antigen receptor T cells, referred to as Car-T cell) therapy is one of the hottest areas of anti-tumor immunity, and it is also a major breakthrough in immunomedicine in the past decade. The effect of Car-T cells in the treatment of hematological malignancies is impressive, and major pharmaceutical companies and inn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K51/04C12N5/0783A61K101/02
CPCA61K51/0406C12N5/0006C12N5/0636
Inventor 霍力方鹏
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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