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Application of Lactobacillus crispatus to preparation of female contraceptives

A technology of Lactobacillus and contraceptives, applied in the field of microorganisms, can solve the problems of affecting human hormone levels, reducing sexual life experience, incomplete contraception, etc., achieving good contraceptive effect, breaking through limitations, and reducing swimming ability

Pending Publication Date: 2020-03-31
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides the application of Lactobacillus crispatus for the preparation of female contraceptives, so as to solve the problem of reducing the experience of sexual life, incomplete contraception, and affecting human hormone levels in conventional contraceptive methods in the prior art. Technical problems such as defects

Method used

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  • Application of Lactobacillus crispatus to preparation of female contraceptives
  • Application of Lactobacillus crispatus to preparation of female contraceptives

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 (Lactobacillus crispatus and semen acquisition)

[0044] 1. Acquisition of Lactobacillus crispatus

[0045]The vaginal secretions of healthy women were collected, and the subjects were required to have no records of vaginal hormones and antibiotics in the near future, no records of cervical treatment within one week, no records of washing within 5 days, and no records of sexual life within 48 hours. Lactobacillus crispatus for subsequent experiments.

[0046] Second, the acquisition of semen

[0047] The semen of healthy men was taken, and the parameters of the sperm of the subjects were normal (including sperm size, sperm count, motile sperm, non-motile sperm, and sperm morphology) and the karyotype was normal. Before sampling, the glans was washed with soap and water, and the semen was obtained by masturbation of the subject, injected into a sterile collection tube, and incubated at 37°C for 25-45 minutes to liquefy.

Embodiment 2

[0048] Example 2 (exploring the influence of Lactobacillus crispatus on sperm in vitro)

[0049] 1. Activation of Lactobacillus crispatus with MRS medium

[0050] (1) Take out the glycerol preservation tube of Lactobacillus crispatus from the -80°C refrigerator, inoculate it in 5mL MRS medium according to 5% of the volume of the fresh medium, and store it at 37°C in 5% CO 2 Cultivate overnight in a constant temperature incubator;

[0051] (2) Take the culture solution in (1), inoculate 5mL of MRS medium according to 5% of the volume of the fresh culture medium, and perform secondary activation of Lactobacillus crispatus to ensure a good physiological state of the bacteria;

[0052] (3) Mix the Lactobacillus crispatus that has been activated twice in step (2) and take 1mL of the bacteria liquid, centrifuge at 6000rpm / min for 3min, discard the supernatant, add 1mL of HS solution to resuspend the bacteria sludge, shake well and centrifuge again to discard the supernatant Clear ...

Embodiment 3

[0062] Embodiment 3 (the effect of Lactobacillus crispatus in animal contraception experiment)

[0063] 1. Establishment of the Rat Reproductive Model

[0064] (1) Female SD rats used in the experiment were divided into groups: negative control group, Lactobacillus crispatus group, 8 in each group, and 10 male SD rats in total;

[0065] (2) Cut the medical absorbent gelatin sponge into a size of 0.5cm×0.5cm, absorb 50μL with a concentration of 1×10 5 The CFU / mL bacterial liquid made the gelatin sponge fully absorb the bacterial liquid and put it into the vagina of the rat. After the vaginal administration of bacteria, the male rats and the female rats were placed in the same cage at a ratio of 1:4 to mate, and the female rats with vaginal plugs were checked at around 9:00 the next morning. Rats were kept separately and bacteria were stopped.

[0066] (3) After 14 days of continuous administration of bacteria, the vaginal secretions of rats were collected to extract vaginal ...

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Abstract

The invention provides an application of lactobacillus crispatus in preparation of female contraceptives. According to the technical scheme, firstly, the influence of lactobacillus crispatus on spermsis studied, and experiments are conducted in an in-vitro co-incubation mode and an animal vaginal administration mode respectively; experimental results show that the lactobacillus crispatus has obvious adhesion to sperms, so that the sperm motility and swimming ability are remarkably reduced. Based on the found new properties of the lactobacillus crispatus, the new application of the lactobacillus crispatus in preparation of female contraceptives is determined. Animal experiments show that when a vaginal suppository containing the lactobacillus crispatus liquid acts on the vagina of a femalemouse, the pregnancy rate of the female mouse can be accurately reduced, and the contraceptive rate can reach about 50%. A lactobacillus crispatus bacterial suspension is safe and non-toxic, the probiotic effect of the lactobacillus crispatus bacterial suspension is combined with reproductive contraception, the limitation of a traditional contraception mode is broken through, and the lactobacillus crispatus bacterial suspension is safe, effective and reversible, can help to adjust genital tract micro-ecological balance of women and is of great significance to reproductive health of women.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and further relates to human microecological technology and contraceptive technology, in particular to the application of Lactobacillus crispatus for preparing female contraceptives. Background technique [0002] Contraception is a technical means to temporarily prevent a woman from becoming pregnant through physical and chemical methods. Traditional contraceptive methods include vasectomy, hormonal contraceptives, and condoms. All of the above methods have certain defects, such as reducing the experience of sexual life, incomplete contraception, and affecting human hormone levels. Therefore, looking for a safer, Effective, reversible, non-hormonal contraceptive methods are of great significance. [0003] The human microbiota has been linked to many health effects, and research focus has recently expanded from the gut to many other organ systems. Published studies have shown that the vag...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61P15/18
CPCA61K35/747A61P15/18
Inventor 陈廷涛王欢李萍
Owner NANCHANG UNIV
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