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A South American tree frog host defense peptide dms-ps2 and its gene and application

A DMS-PS2, host defense peptide technology, applied in the field of biomedicine, can solve the problems of weak research on active ingredients and pharmacological properties, and achieve the effect of inhibiting the growth of bacteria and fungi

Active Publication Date: 2022-04-29
WENZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although our country has a long history of using amphibians, they are all used as medicines as a whole, and the research on their active ingredients and pharmacological properties is relatively weak.

Method used

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  • A South American tree frog host defense peptide dms-ps2 and its gene and application
  • A South American tree frog host defense peptide dms-ps2 and its gene and application

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Embodiment Construction

[0020] The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0021] 1. Preparation and amino acid sequence determination of South American tree frog host defense peptide DMS-PS2

[0022] 1. Collection of South American tree frog skin secretions

[0023] The live South American tree frog was cleaned with water and placed in a cylindrical sample bottle with a diameter of 10 cm and a height of 10 cm. The surface of the tree frog's back was rubbed, and the frog's back foam liquid was rinsed with 100 ml of deionized water. The secreted liquid was quick-frozen in liquid nitrogen and freeze-dried with a freeze-drying machine, and the freeze-dried powder was stored at -80°C.

[0024] 2. Isolation and purification of South American tree frog host defense peptide DMS-PS2

[0025] Using the lyophilized powder of the excretion collected above as raw material, the South American tree frog host defense peptide DMS-PS2 was purifie...

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Abstract

The invention discloses a South American tree frog host defense peptide DMS-PS2 and its gene and application, belonging to the field of biomedicine. The host defense peptide DMS‑PS2 is a polypeptide consisting of 28 amino acids with a molecular weight of 2953.5 Daltons. The gene encoding the DMS‑PS2 precursor peptide (GenBank: LT718217.1) consists of 346 nucleotide sequences, of which the 136‑228th nucleotide sequence is the gene encoding the DMS‑PS2 mature peptide, which is further post-translationally modified to obtain Mature peptide DMS‑PS2. The DMS-PS2 of the present invention has broad-spectrum antibacterial activity, has strong antibacterial effect on free bacteria and bacterial biofilm, and its mode of action is to directly act on bacterial biofilm to make bacterial plasma membrane broken, so it is not easy to produce bacterial drug resistance It provides a theoretical basis for the development of therapeutic drugs against infectious diseases and coating drugs for medical devices.

Description

technical field [0001] The invention relates to a South American tree frog host defense peptide DMS-PS2 and its application, belonging to the technical field of biomedicine. Background technique [0002] Antibiotics are widely used to treat infections caused by susceptible microorganisms, usually bacteria or fungi. However, due to people's irrational use and even abuse of these "traditional antibiotics", many bacteria have developed resistance to them. So people began to look for new antimicrobial alternatives. Studies in recent years have found that polypeptide antibiotics not only have broad-spectrum antimicrobial activity, but also have incomparable advantages over "traditional antibiotics": at very low concentrations, they can quickly kill various bacteria (including clinically resistant bacteria). It also has inhibitory effect on fungi and viruses; it is not easy to produce drug resistance to microorganisms; it is effective for both local infection and systemic infect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C12N15/12A61K38/17A61P31/04A61P31/10
CPCC07K14/47A61P31/04A61P31/10A61K38/00Y02A50/30
Inventor 高艺恬吴迪吴明江宋昕宇佟海滨张旭
Owner WENZHOU UNIVERSITY
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