Lyoprotectant, fermentation agent, preparation method and application thereof
A technology of freeze-drying protective agent and starter, applied in the field of starter and its preparation, and freeze-drying protectant
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[0059] According to one aspect of the present invention, a kind of preparation method of above-mentioned lyoprotectant, described preparation method comprises the following steps:
[0060] Mixing the raw materials evenly and sterilizing to obtain the freeze-dried protective agent;
[0061] The preparation method of the freeze-drying protective agent provided by the present invention comprises the following steps: uniformly mixing all raw materials and sterilizing to obtain the freeze-drying protective agent. The above preparation method has the advantage of simple preparation process.
[0062] In a preferred embodiment of the present invention, the preparation method specifically includes the following steps:
[0063] (a), dissolving skim milk powder and tamarind polysaccharide in part of water, and sterilizing to obtain mixed solution A;
[0064] (b), dissolving polydextrose in the remaining water, and sterilizing to obtain a polydextrose solution;
[0065] (c), under asep...
Embodiment 1
[0094] A starter, the starter is mainly made from a freeze-drying protectant and Lactobacillus plantarum Zhang-LL;
[0095] The preparation method of described starter comprises:
[0096] (1), prepare lyoprotectant:
[0097] The lyoprotectant comprises the following components in terms of mass percentage: 10% skim milk powder, 0.1% tamarind polysaccharide, 2% polydextrose, and 87.9% water;
[0098] The preparation method comprises the following steps:
[0099] (a) Dissolve skim milk powder and tamarind polysaccharide in part of the water, sterilize at 115°C for 7 minutes by high-pressure steam, take it out quickly after the temperature drops below 80°C, and obtain mixed solution A;
[0100] (b), dissolving polydextrose in the remaining water, and filtering and sterilizing through a 0.22 μm filter membrane to obtain a polydextrose solution;
[0101] (c), under aseptic conditions, mix the mixed solution A with the polydextrose solution to obtain a lyoprotectant;
[0102] (2)...
Embodiment 2
[0113] A starter, the starter is mainly made from a freeze-drying protectant and Lactobacillus plantarum Zhang-LL;
[0114] The preparation method of described starter comprises:
[0115] (1), prepare lyoprotectant:
[0116] The lyoprotectant comprises the following components in terms of mass percentage: 15% of skim milk powder, 0.3% of tamarind polysaccharide, 5% of polydextrose, and 79.7% of water;
[0117] The preparation method comprises the following steps:
[0118] (a) Dissolve skim milk powder and tamarind polysaccharide in some water, sterilize at 115°C for 15 minutes by high-pressure steam, and take it out quickly after the temperature drops below 80°C to obtain mixed solution A;
[0119] (b), dissolving polydextrose in the remaining water, and filtering and sterilizing through a 0.22 μm filter membrane to obtain a polydextrose solution;
[0120] (c), under aseptic conditions, mix the mixed solution A with the polydextrose solution to obtain a lyoprotectant;
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