Culture medium for saprophiticnematode in infected wood of pine wood nematodes and preparation method and application of culture medium

Abstract

The invention discloses a culture medium for saprophiticnematode in infected wood of pine wood nematodes and a preparation method and application of the culture medium and belongs to the technical field of culture of saprophiticnematode. The formula of the saprophiticnematode culture medium comprises 20-30g / L of pine wood bits, 10-20g / L of glucose and 20-30g / L of agar. After high-pressure steam sterilization, cooling and curing, the culture medium is inoculated with saprophiticnematode, the saprophiticnematode is cultured for 10 days or longer in an incubator at 25 DEG C, and the saprophiticnematode is collected, wherein the pine wood bits are wood bits of xylem of an original pinus tree variety from which the saprophiticnematode can be separated. Compared with a PDA (potato dextrose agar)culture medium which is full of botrytis cinerea for culturing the saprophiticnematode and takes 22-30 days to obtain a great deal of saprophiticnematode, the culture medium is capable of shorteningthe culture time by a half or greater, increasing the speed of indoor culture of the saprophiticnematode and improving the culture efficiency; and in addition, the culture medium is simple in preparation step, expected raw materials are easy to obtain and low in price, and large-scale production and use can be easily achieved.

Description

technical field

[0001] The invention belongs to the technical field of saprophytic nematode culture, and in particular relates to a culture medium for saprophytic nematodes of pine wood nematode disease and its preparation method and application. Background technique

[0002] Saprophytic nematodes refer to the nematodes that feed on sugar and protein decomposition products of bacterial action (Liu Nanxin et al., 1982), or feed on decayed organic debris. Saprotrophic nematodes are present in large numbers in the soil and are often seen in and around plant roots and dead and decaying tissue in the underground or aerial parts of plants. Saprophytic nematodes in diseased and dead wood of pine wood nematode are also extremely common (Ren-E et al, 2010; Li Xiangkang et al, 2010; Wang Xinrong et al, 2008), but people have little knowledge about the morphology, physiology and ecology of saprophytic nematodes in dead wood of pine wood nematode. Research is still limited, and one of ...

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