Method for evaluating toxicity of total sediments by utilizing daphnia magna

An evaluation method and sediment technology, which can be used in biological testing, material inspection products, measuring devices, etc., can solve the problems of reducing the toxic concentration of elutriation water, underestimating the actual risk of polluted sediments, etc., and achieve good holding capacity and mild preparation process. Effect

Pending Publication Date: 2020-04-10
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the actual risk of contaminating the sediment is underestimated because hydrophobic contaminants bound to sediment particles (or carbon particles) are removed during centrifugation / filtration, resulting in a decrease in toxic concentrations in the elutriation water

Method used

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  • Method for evaluating toxicity of total sediments by utilizing daphnia magna
  • Method for evaluating toxicity of total sediments by utilizing daphnia magna
  • Method for evaluating toxicity of total sediments by utilizing daphnia magna

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A method for evaluating the toxicity of whole sediments utilizing Daphnia magna, comprising the following steps:

[0029] 1. Freeze-dry the sediment of the river channel with a freeze dryer, sieve it, and obtain a sediment sample;

[0030] 2. Weigh 5g sediment sample, add 50mL calcium chloride (1%, m / v) solution, shake at 150rpm for 4h, and centrifuge at 7000rpm to remove the supernatant, then use deionized water to wash three times to remove soluble Then add 30mL acetone and 30mL dichloromethane respectively, ultrasonically extract for 10min three times, centrifuge at 7000rpm, and then use deionized water to wash three times to remove organic pollutants and remaining organic solvents. Dry in a fume hood to obtain blank deposits in situ;

[0031] 3. Mix the sediment sample and the in-situ blank sediment to obtain a group of mixed samples with a total dry weight of 0.5 g and different ratios of sediment samples, wherein the mass ratios of the sediment samples in each gr...

Embodiment 2

[0038] A method for evaluating the toxicity of whole sediments utilizing Daphnia magna, comprising the following steps:

[0039] 1. Take the soil and use a freeze dryer to freeze-dry it and sieve it to obtain a sediment sample;

[0040] 2. Weigh 10g sediment sample, add 50mL calcium chloride (2%, m / v) solution, shake at 300rpm for 8h, and centrifuge at 12000rpm to remove the supernatant, then use deionized water to wash three times to remove soluble state of heavy metals and remaining calcium chloride. Then add 30mL of acetone and 30mL of dichloromethane, ultrasonically extract three times for 30min, centrifuge at 12000rpm, then use deionized water, wash three times to remove organic pollutants and remaining organic solvents, and dry in a fume hood to obtain in-situ blank deposits;

[0041] 3. Mix the sediment sample and the in-situ blank sediment sample to obtain a group of mixed samples with a total dry weight of 1.5 g and different ratios of sediment samples, wherein the rat...

Embodiment 3

[0048] A method for evaluating the toxicity of whole sediments utilizing Daphnia magna, comprising the following steps:

[0049] 1. Take the sediment of the river course and use a freeze dryer to freeze-dry and sieve to obtain a sediment sample;

[0050] 2. Weigh 5g sediment sample, add 50mL calcium chloride (1%, m / v) solution, shake at 150rpm for 4h, and centrifuge at 7000rpm to remove the supernatant, then use deionized water to wash three times to remove soluble Then add 30mL acetone and 30mL dichloromethane respectively, ultrasonically extract for 10min three times, centrifuge at 7000rpm, and then use deionized water to wash three times to remove organic pollutants and remaining organic solvents. Dry in a fume hood to obtain blank deposits in situ;

[0051] 3. Mix the sediment sample and the corresponding in-situ blank sediment sample to obtain a group of mixed samples with a total dry weight of 1 g and different ratios of sediment samples, wherein the ratios of sediment ...

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Abstract

The invention provides a method for evaluating toxicity of total sediments by using daphnia magna, which comprises the following steps: 1) taking sediments, freeze-drying, grinding and sieving to obtain a sediment sample; 2) detoxicating the sediment sample to obtain an in-situ blank sediment sample, and diluting the corresponding sediment sample by using the in-situ blank sediment sample to obtain a plurality of groups of mixed samples with equal mass and different sediment sample concentrations; 3) respectively stirring and uniformly mixing the mixed samples with a sodium alginate solution to prepare a plurality of groups of standby mixed solutions; 4) respectively adding the plurality of groups of standby mixed solutions into the plurality of groups of calcium chloride solutions at a constant speed, cross-linking to form immobilized total sediments, rinsing, transferring into standard dilution water containing daphnia magna, continuously culturing, and periodically recording the death number of daphnia magna; and 5) calculating the lethal medium concentration LC50 of the sediments according to the biological effect of the daphnia magna and the dilution ratio of the sediments, and judging the toxicity grade of the sediments. The method for determining the toxicity of the full sediments by utilizing the daphnia magna is simple, rapid and sensitive in determination.

Description

technical field [0001] The invention relates to a method for evaluating the toxicity of whole sediments using daphnia magna, belonging to the field of ecological safety evaluation. Background technique [0002] After the pollutants are discharged into the river, they are finally deposited in the sediments. Under appropriate conditions, the pollutants will be released into the overlying water again, making the sediments a secondary pollution source in the water environment. Therefore, the Whole-Sediment-Toxicity test (WST) is very important in the ecological safety assessment of river courses. WST experiments usually use a 10-day amphiped (Hyalella Azteca) acute toxicity test or a 4-6 week chironomus (Chironomus tentans) chronic toxicity test to measure sediment ecotoxicity, which requires a lot of time and manpower. Daphnia magna (Daphnia magna) is one of the most commonly used model organisms in aquatic toxicity tests. Its growth rate is fast, its reproduction cycle is sho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/5014G01N33/5085
Inventor 孙丽伟张静裴舟韬王文强徐柔柔张萌张李凌
Owner SOUTHEAST UNIV
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