Applications of ECG is used as muscle-strengthening functional component

A functional ingredient and muscle-building technology, applied in food science and other fields, can solve problems such as toxic side effects and limited effects, and achieve the effects of maintaining muscle homeostasis, delaying muscle atrophy, and enhancing muscle strength

Pending Publication Date: 2020-04-14
HUNAN AGRICULTURAL UNIV
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many studies have attempted to reduce muscle loss through drug intervention, these drugs have limited effects and have certain toxic and side effects (Dugdale HF, Hughes D C, Allan R, et al. The role of resveratrol on Skeletal muscle cell differentiation and myotube hypertrophy during glucose restriction[J]. Mol Cell Biochem, 2018, 444(1-2): 109-123.; Zismanov V, Chichkov V, Colangelo V, et al. Phosphorylation of eIF2alpha Is a Translational Control Mechanism Re Muscle Stem Cell Quiescence and Self-Renewal[J]. Cell Stem Cell, 2016, 18(1): 79-90.)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Applications of ECG is used as muscle-strengthening functional component
  • Applications of ECG is used as muscle-strengthening functional component
  • Applications of ECG is used as muscle-strengthening functional component

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: ECG can enhance C2C12 cell differentiation

[0031] In order to evaluate the effect of ECG on enhancing the differentiation activity of C2C12 cells, two concentrations of 10 μM and 20 μM (specifically referring to the concentration of the active functional component ECG) were selected to act on C2C12 cells for 4 days, respectively, and the effect on myotube phenotype and differentiation markers ( The specific evaluation items were the number, length, and diameter of myotubes, and the relative expression levels of myogenic regulatory factors MyoD, MyoG, and myosin heavy chain (MyHC) mRNA. Figure 1A is the topography of myotubes after continuous culture of C2C12 cells differentiated with different concentrations of ECG for 4 days, including the blank control. Figure 1B shows the detection of myotube differentiation markers when the cells were differentiated to the 4th day, the relative expression levels of MyoD, MyoG and MyHC mRNAs in sequence. Overall, the ex...

Embodiment 2

[0034] Example 2: ECG can change the surface morphology and mechanical properties of myotube cells

[0035] In order to explore the influence of ECG on the morphology and mechanical properties of myotube cells differentiated to the mature stage, we selected ECG at concentrations of 0 and 10 μM to act on the cells differentiated to the terminal stage (day 5) for atomic force microscopy scanning. image 3 A and Figure 3B are the three-dimensional topography of mature myotube cells and the Young's modulus of each part of the myotube when the control group (0 μM) and the experimental group (10 μM ECG) were treated with cells to differentiate to day 5 respectively. Figure 3C is the statistical results of ECG on the height, stiffness, adhesion and Young's modulus of C2C12 differentiated to the fifth day. The height of mature myotubes increased from 2.226±0.122 (μm) to 6.400±0.338 (μm) after continuous treatment with 10μM ECG for 5 days, an increase of about 178.5%. On the other han...

Embodiment 3

[0036] Example 3: Proteomic analysis of the possible molecular mechanism of ECG regulation of myoblast differentiation

[0037] In order to further explore the mechanism by which ECG promotes the differentiation of C2C12 cells, and to find related protein molecules and signaling pathways that ECG enhances myotube differentiation, we used liquid chromatography tandem mass spectrometry to analyze the early (0 day) and middle (2 day) stages of ECG on C2C12 cells. ) and the final stage (5 days) for full proteomic analysis at three time points, the set groups include: undifferentiated group, 2-day differentiation blank control group, 2-day differentiation ECG treatment group (10 μM), 5-day differentiation blank control group And differentiate the 5-day ECG-treated group (10 μM). After extracting proteins from these five groups of cells for mass spectrometry detection and database comparison, a total of 179 common genes were detected. Genesis 1.8.1 software was used to perform SOM ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to applications of ECG as a muscle-strengthening functional component in food, and provides a green tea ECG-containing dietary nutrition supplement with a muscle-strengthening effect. The main functional component of the supplement is pure natural botanical green tea extract epicatechin gallate (ECG). The ECG can promote muscle regeneration, inhibit muscle protein degradation, inhibit oxidative damage of muscle protein, promote survival of muscle cells and improve athletic ability, and has extremely wide application value.

Description

technical field [0001] The invention belongs to the technical field of food, specifically relates to the application of ECG as a muscle-building functional component in food, and provides a dietary nutritional supplement containing green tea ECG with muscle-building effects. Background technique [0002] The loss of skeletal muscle mass and strength is mostly caused by conditions such as aging, muscle atrophy caused by chronic diseases, cachexia and sarcopenia. Fatigue and insulin resistance, which lead to increased mortality in patients (Engler A J, Carag-Krieger C, Johnson C P, et al. Embryonic cardiomyocytes beat best on a matrix with heart-like elasticity: scar-like rigidity inhibits beating[J]. J CellSci, 2008, 121(Pt 22): 3794-802.; Varga B, Martin-Fernandez M, Hilaire C, et al. Myotube elasticity of an amyotrophic lateral sclerosis mouse model[J]. Sci Rep, 2018, 8(1 ): 5917.). With age, after the age of 50, muscle mass declines at a rate of 1%-2% per year (Li P, Liu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105
CPCA23L33/105A23V2002/00
Inventor 张盛李鹏辉刘昌伟屈志豪袁斌肖文军刘仲华
Owner HUNAN AGRICULTURAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap