Solid-phase carrier device for oligonucleotide synthesis and selective modification method of device

Abstract

The invention discloses a solid-phase carrier device for oligonucleotide synthesis and a selective modification method of the device, and provides the solid-phase carrier device for oligonucleotide synthesis and the selective functional modification method of the device. The solid-phase carrier device is a silicon wafer, and includes primary pores and secondary pores, and the primary pores are arranged in a reasonable array and are through holes embedded in the secondary pores. The selective modification method comprises the following steps: applying a first group of molecules to the surface of the silicon wafer, performing uniform spin-coating at the surface of the silicon wafer and the secondary pore area by using a glue homogenization machine and centrifugal force to protect the molecules from having a functionalization reaction, and performing modification in the primary pore area by using a functionalized reagent. The method has the advantages of low costs, simple experimental operation, easy realization and high modification efficiency, and avoids complicated experimental operation and technological process of a photolithography technology; and the design of the selective modification method of the solid-phase carrier can solve the problem of positioning modification of a microfluidic chip solid-phase carrier device in a new generation DNA synthetic technology.

Description

technical field [0001] The invention relates to the field of biosynthesis, in particular to a solid-phase carrier device for oligonucleotide synthesis and a selective modification method thereof. Background technique [0002] With the development of society and the advancement of science and technology, synthetic biology technology has become an emerging strategic international frontier technology, and its rapid development has put forward an increasingly urgent demand for gene synthesis capabilities. Since 1960, the technology of chemical in vitro synthesis of oligonucleotides has been developed until now. The throughput, efficiency and performance of DNA synthesizers are the key factors affecting the cost and quality of DNA synthesis. At this stage, most of the first-generation DNA synthesizers are based on column synthesis equipment. In the latest research progress at home and abroad, the research on a new generation of DNA synthesis technology and instruments based on mi...

AI Technical Summary

Problems solved by technology

However, the use of porous glass as a solid phase carrier has disadvantages such as large reagent consumption and high synthesis cost.

The new-generation DNA synthesizer based on the microfluidic chip integrates micro reagents into the microfluidic chip for reaction, which reduces the cost, but the modification of its solid phase carrier mostly uses photolithography technology, which needs to go through glue coating, pre-baking, and exposure. , development, film hardening, etching and degumming and other seven steps, the process is more complicated

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