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Salt-tolerant growth-promoting bacterial strain JP-JH capable of alleviating plant salt damage and applications thereof

A JP-JH, growth-promoting bacteria technology, applied in the field of microorganisms, to achieve the effects of reducing production costs, alleviating plant salt damage, and solving cost increases

Active Publication Date: 2020-05-05
中国科学院遗传与发育生物学研究所农业资源研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the strains targeting Kushneria indalinina are limited to systematic taxonomic identification and basic salt tolerance characteristics at the gene level, and no research has yet pointed out that strains belonging to Kushneria indalinina have the ability to promote plant growth

Method used

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  • Salt-tolerant growth-promoting bacterial strain JP-JH capable of alleviating plant salt damage and applications thereof
  • Salt-tolerant growth-promoting bacterial strain JP-JH capable of alleviating plant salt damage and applications thereof
  • Salt-tolerant growth-promoting bacterial strain JP-JH capable of alleviating plant salt damage and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Isolation and purification of salt-tolerant growth-promoting bacteria JP-JH

[0026] Sample source: Rhizospheric soil of Suaeda salsa in saline-alkali land in Haixing County, Cangzhou City, Hebei Province, China.

[0027] LB medium (solid): tryptone 10g / L, yeast extract 5g / L, sodium chloride 50g / L, agar 20g / L, use 5M / L NaOH to adjust the pH value, sterilize by high pressure steam at 121℃ for 30min .

[0028] LB medium (liquid): Tryptone 10g / L, yeast extract 5g / L, sodium chloride 50g / L, after adjusting the pH value with 5M / L NaOH, sterilize under high pressure steam at 121°C for 30min.

[0029] Separation and purification steps: 1) collect the root system of Suaeda salsa in Haixing County, Cangzhou City, Hebei Province, China, shake off the surface soil of the root system, put it into a sterile 50mL centrifuge tube, add sterile water and vortex to obtain the rhizosphere soil stock solution ; 2) leave it still, take an appropriate amount of supernatant, and u...

Embodiment 2

[0030] Example 2: Identification of salt-tolerant growth-promoting bacteria JP-JH

[0031] 1) Identification of biological characteristics

[0032] The colony of the salt-tolerant growth-promoting bacteria JP-JH on the LB medium was orange-yellow protruding, and the edge of the colony was slightly transparent ( figure 1 ).

[0033] 2) System classification identification

[0034] The present invention adopts the kit TIANamp Bacteria DNA Kit (Tiangen, Beijing) and extracts the salt-tolerant growth-promoting bacteria JP-JH genome DNA according to its operation steps. The 16S rDNA sequence of strain JP-JH was amplified by primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-TACGGCTACCTTGTTACGACTT-3'). The PCR reaction system was 50 μL, which contained 10 μL EasyTaq buffer, 5 μL dNTPs, 0.5 μL primer 27F, 0.5 μL primer 1492R, 1.5 μL DNA template, 2 μL Easy Taq DNA polymerase, and finally added sterile ultrapure water to 50 μL. The PCR reaction program was: (i) pre-denaturatio...

Embodiment 3

[0036] Example 3: Evaluation of Salt-tolerant and Alkali-resistant Ability of Salt-tolerant Growth-Promoting Bacteria JP-JH

[0037]Pick a single colony of the strain JP-JH on the solid LB medium, inoculate it in 50mL liquid LB medium, cultivate it at 28°C and 150r / min for 48h, and then inoculate it in NaCl solution containing 1%, 5%, 10%, 15% and 20%, and the LB solid medium combined with the pH value of 7.0, 8.0 and 9.0 for streak culture. Grow in the medium of ~9.0.

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Abstract

The invention discloses a salt-tolerant growth-promoting bacterial strain JP-JH capable of alleviating plant salt damage and applications thereof. The strain belongs to Kushneria indalinina, and is deposited with the China Center for Type Culture Collection on December 12th, 2019 and assigned the accession number CCTCC M 20191039. The strain has strong salt and alkali resistance; the concentration, tolerated by the growth of the strain, of NaCl is 1-15%(w / v), and the tolerant pH value is 7.0-9.0; and IAA can be secreted without the induction of L-tryptophan. In addition, the strain can achievesignificant promotion effects on the growth (leaf length and fresh weight on the ground) of wheat in living and inactivated states and under salt-free stress and salt stress conditions and can obviously alleviate salt damage to the wheat under the salt stress conditions. Thus, compared with reported salt-tolerant strains, the bacterial strain JP-JH can reasonably reduce the requirements for the cultivation, storage, transportation and colonization of the strains, so that production costs can be reduced, and convenient promotion and application can be achieved.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a salt-tolerant growth-promoting bacterium for relieving plant salt damage and its application. Background technique [0002] Saline-alkali land is the main soil type in my country's medium and low-yield fields. Rational development and utilization of these saline-alkali land resources is of great significance for ensuring my country's national food security, promoting sustainable agricultural development, improving the ecological environment, and promoting coordinated regional economic and social development. The biological improvement measures of saline-alkali land can overcome some limitations of physical, chemical, and engineering improvement measures, and also meet the current needs of green development of saline-alkali land. Relatively mature bio-improvement measures are mainly plant-based measures, including planting salt-tolerant trees (such as Eleuthero jujube, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00C05F11/08C12R1/01
CPCA01N63/00C05F11/08C12N1/20C12N1/205C12R2001/01
Inventor 王新珍刘小京胡春胜刘彬彬
Owner 中国科学院遗传与发育生物学研究所农业资源研究中心
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