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Application of fraxetin in anti streptococcus agalactiae infection of aquatic animals

A technology for Streptococcus lactis and aquatic animals, which is applied in the fields of application, antibacterial drugs, animal feed, etc., can solve the problems of drug-resistant bacteria, poor treatment effect, and damage to the healthy breeding of tilapia, and achieve low dosage and high therapeutic effect Good, not easy to drug resistance effect

Inactive Publication Date: 2020-05-08
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The treatment of tilapia agalactiae streptococcosis mainly depends on chemical antibacterial drugs, which not only has poor treatment effect but also leads to the emergence of drug-resistant bacteria, which seriously damages the healthy farming of tilapia

Method used

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  • Application of fraxetin in anti streptococcus agalactiae infection of aquatic animals
  • Application of fraxetin in anti streptococcus agalactiae infection of aquatic animals
  • Application of fraxetin in anti streptococcus agalactiae infection of aquatic animals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: Minerin is measured to the minimum inhibitory concentration of Streptococcus agalactiae

[0013] The Streptococcus agalactiae strain PBSA0903 involved in the present invention was donated by Dr. Guo Weiliang of Hainan University (Meng Aiyun, Zhou Yongcan, Hu Wenting, Wu Mengxiao, Wang Shifeng, Xie Zhenyu, Guo Weiliang. The influence of temperature on the hemolytic value of 14 strains of Streptococcus agalactiae[J]. Journal of Hainan University (Natural Science Edition), 2016,34(02):170-176.), preserved by the Laboratory of Aquatic Animal Pharmacology and Drug Residue Control Technology, Yangtze River Fisheries Research Institute.

[0014] The minimum inhibitory concentration of afracetin on PBSA0903 was determined by the broth microdilution method recommended by CLSI. Proceed as follows:

[0015] (1) Dilute the prepared fencetrin (purchased from Shanghai Yuanye Biotechnology Co., Ltd., product number: B20990) in 96-well cell culture plates, and the concen...

Embodiment 2

[0017] Example 2: Determination of the inhibitory effect of afracetin on sortase A of Streptococcus agalactiae by energy transfer resonance method

[0018] Sortase A involved in the present invention (references: Khare, B., et al., Preliminarycrystallographic study of the Streptococcus agalactiae sortases, sortase A and sortase C1.Acta Crystallogr Sect F Struct Biol Crystal Commun, 2010.66 (Pt 9): p.1096-100.) is preserved by the Laboratory of Aquatic Animal Pharmacology and Drug Residue Control Technology of Yangtze River Fisheries Research Institute, and Streptococcus agalactiae PBSA0903 is preserved by Yangtze River Fisheries Research Institute. The specific test steps are as follows: Add 3 μL of 6 mg / mL sortase A protein stock solution and 10 μL of 200 μM fluorescent substrate (Dabcyl-QALPETGEE-Edans) to a black opaque 96-well culture plate, and then add 10240 μg / mL of Frucetin (purchased from Shanghai Yuanye Biotechnology Co., Ltd., product number: B20990) stock solution ...

Embodiment 3

[0019] Example 3: Effects of afracetin on the adhesion function of S. agalactiae mediated by sortase A

[0020] Dilute fibrinogen and fibronectin mother solution to 4 μg / mL with PBS, add 200 μL to a 96-well cell culture plate, and incubate overnight at 4°C. The next day, the liquid in the plate was discarded, washed 3 times with 200 μL PBS, and 5% BSA was added to each well to block in a 37° C. incubator for 2 hours. Then discard the liquid and wash 3 times with PBS for later use. Pick a single colony of Streptococcus agalactiae PBSA0903 strain and inoculate it in 2 mL of BHI medium, culture it at 37°C for 8 hours, expand it into 100 mL of medium, and add 32, 16, 8, 4, 2, 0 μg / mL of Fraxin, continue to culture to OD 600nm When =0.5, the cells were collected by centrifugation. The cells were washed 3 times with PBS and then adjusted to OD 600nm Standby for 1.0. Add 100 μL of the treated bacterial solution to the sealed 96-well cell culture plate, and incubate in a 37°C inc...

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Abstract

The invention discloses application of fraxetin in anti streptococcus agalactiae infection of aquatic animals. The adhesion effect of streptococcus agalactiae can be reduced through inhibiting the activity of sortase A by fraxetin, so as to reduce the pathogenicity of streptococcus agalactiae to aquatic animals. According to the present invention, the infection model of streptococcus agalactiae oftilapia confirms that fraxetin can play a therapeutic role in the infection of streptococcus agalactiae of tilapia. Fraxetin is the main chemical component of the bark of ash trees, has no toxic sideeffects and residues, and can be used as a pharmaceutical preparation and a feed additive for the prevention and treatment of streptococcus agalactiae of aquatic animals.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to the application of afracetin in the preparation of medicines for preventing or treating streptococcus agalactiae infection in aquatic animals, or as an aquatic feed additive for resisting streptococcus agalactiae infection. Background technique [0002] Ash tree, also known as green palm and white wattle, belongs to the deciduous trees of Oleaceae. It has a long history of cultivation in my country and is widely distributed in areas. Among them, the bark of ash tree is mostly used for medicinal purposes. White wax bark is slightly warm in nature, pungent in taste, non-toxic; returns to the liver, gallbladder, and large intestine meridian. It is mainly used to treat malaria, irregular menstruation and head sores in children. Modern medical research shows that ash cortex has the functions of anti-inflammatory and analgesic, affecting urine volume and excretion of u...

Claims

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Application Information

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IPC IPC(8): A61K31/366A61P31/04A23K50/80A23K20/195
CPCA61K31/366A61P31/04A23K50/80A23K20/195
Inventor 董靖艾晓辉刘永涛胥宁杨秋红杨移斌周顺
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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