Salt-tolerant, heterotrophic and aerobic nitrifying bacterium strain, culture method, bacterial liquid and application
A nitrifying bacteria strain and heterotrophic nitrification technology, applied in the field of salt-tolerant heterotrophic aerobic nitrifying bacteria strains, can solve problems such as poisoning and achieve good market application prospects
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[0038] Example one
[0039] The isolation and enrichment screening steps of heterotrophic nitrification strains are as follows:
[0040] (1) Activated sludge was collected from Tianjin PetroChina Dagang Petrochemical Company, and the sludge sample was inoculated into a sterilized nitrification medium by dilution and plate coating. The initial NH 4 + -N concentration is 100mg / L, the nitrification medium formula is as follows (g / L): ammonium chloride 0.3, sodium acetate 1, disodium hydrogen phosphate 0.2, potassium chloride 0.1, sodium chloride 10, magnesium sulfate 0.05, and 1mL Trace element solution, pH 7.0, culture on a shaker at 30°C to obtain isolated strains;
[0041] (2) Put the strains isolated in (1) into the culture medium for NH 4 + -N removal experiment, NH 4 + The strain with the highest N removal rate is the desired strain.
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[0042] Example two
[0043] Molecular identification of strains
[0044] Thirty strains were selected from the activated sludge, and the HQXH-21 strain with the best ammonia nitrogen removal effect was selected for 16S r RNA identification, and 16S r RNA was used to identify Pseudomonas ( Pseudomonas sp. ) And named Pseudomonas sp. HQXH-21, the single colony of this strain in LB medium is regular round, moist, yellow in color, opaque, and viscous. See the attachment for the sequence list in the 16S r RNA identification of the strain of the present invention.
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[0045] Example three
[0046] Pseudomonas sp. The cultivation method of HQXH-21
[0047] The above-mentioned isolated strain was cultured on a nitrification medium, and its composition was: ammonium chloride 0.3g / L, sodium acetate 1g / L, disodium hydrogen phosphate 0.2g / L, potassium chloride 0.1g / L, and sodium chloride 10g / L, magnesium sulfate 0.05g / L and 1mL trace element solution; culture temperature is 30℃; pH is 7;
[0048] Inoculate the nitrification medium at 5% of the inoculum, and cultivate in a shaker at 37°C and 150 r / min; replace the fresh heterotrophic nitrification medium at a volume ratio of 10% every 48 hours; continuously cultivate for more than 30 hours.
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