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A method for improving lignocellulose enzymatic hydrolysis and saccharification efficiency with glucose-assisted ball milling and its application

A technology for lignocellulase and saccharification efficiency, applied in the field of biomass utilization, can solve problems such as low efficiency of enzymatic hydrolysis and saccharification, and achieve the effects of avoiding equipment corrosion, shortening process flow and simple operation.

Active Publication Date: 2021-09-24
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the compact structure of lignocellulosic raw materials, they are highly resistant to enzymes, resulting in low enzymatic saccharification efficiency

Method used

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  • A method for improving lignocellulose enzymatic hydrolysis and saccharification efficiency with glucose-assisted ball milling and its application

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Embodiment 1

[0051] A method for improving the enzymatic hydrolysis and saccharification efficiency of eucalyptus by assisting ball milling with glucose, the specific steps are as follows:

[0052] (1) Preparation of eucalyptus wood powder: After pulverizing eucalyptus sapwood wood chips with a medicine grinder, sieve the eucalyptus wood powder with 40-60 meshes.

[0053](2) Degreasing treatment: the eucalyptus powder obtained by crushing is wrapped with 200 mesh gauze and placed in the extraction tube, acetone and ethanol are added into the extraction tube according to the volume ratio of 2:1, and the mixed solution must completely cover the eucalyptus powder (solid The liquid ratio is 1.2:10, g / ml), and after the wood powder is completely soaked, the eucalyptus wood powder is extracted by circulating the heating and cooling extract (heating temperature is 75°C, and the duration is 6h), and the eucalyptus raw material in the gauze is taken out, 50 Dry at ~70°C to obtain the degreased euca...

Embodiment 2

[0064] A method for improving the enzymatic hydrolysis and saccharification efficiency of eucalyptus by assisting ball milling with glucose, the specific steps are as follows:

[0065] (1) Preparation of eucalyptus wood powder: After pulverizing eucalyptus sapwood wood chips with a medicine grinder, sieve the eucalyptus wood powder with 40-60 meshes.

[0066] (2) Degreasing treatment: Wrap the eucalyptus wood powder obtained by crushing with 250 mesh gauze and place it in the extraction tube, add acetone and ethanol into the extraction tube according to the volume ratio of 2:1.2, and the mixed solution must completely cover the wood powder (solid-liquid The ratio is 1.5:10, g / ml), after the wood powder is completely soaked, extract the eucalyptus wood powder by circulating heating and cooling the extract (80°C, for 8h), take out the eucalyptus raw material in the gauze, and dry it (50~ 70°C) to obtain degreased eucalyptus raw material.

[0067] (3) Dilute alkali pretreatment:...

Embodiment 3

[0073] A method for improving the enzymatic hydrolysis and saccharification efficiency of eucalyptus by assisting ball milling with glucose, the specific steps are as follows:

[0074] (1) Preparation of eucalyptus wood powder: After pulverizing eucalyptus sapwood wood chips with a medicine grinder, sieve the eucalyptus wood powder of 60 to 80 meshes.

[0075] (2) Degreasing treatment: the eucalyptus powder obtained by crushing is wrapped with 220 mesh gauze and placed in the extraction tube, acetone and ethanol are added into the extraction tube according to the volume ratio of 2:1.5 (solid-liquid ratio is 1.5:10, g / ml), the mixed solution must completely cover the wood powder, after the wood powder is completely soaked, extract the eucalyptus wood powder by circulating the heating and cooling extract (85°C, for 10 hours), take out the eucalyptus raw material in the gauze, and dry (50~ 70°C), to obtain degreased eucalyptus raw material.

[0076] (3) Dilute alkali pretreatme...

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Abstract

The invention discloses a method for improving lignocellulose enzymatic hydrolysis and saccharification efficiency by assisting ball milling with glucose and an application thereof. The method comprises the following steps: (1) pulverizing the lignocellulosic raw material to obtain lignofiber powder; (2) extracting the lignocellulosic powder with an organic solvent and drying it to obtain degreased lignocellulosic raw material; (3) degreasing The final lignocellulosic raw material is mixed with sodium hydroxide solution evenly and then reacted at 110-180°C. After the reaction is completed, cool, filter with suction, take the filter residue, wash and dry to obtain a solid product; (4) the solid product After being mixed with glucose, ball milling is carried out to obtain a ball milling product; (5) the ball milling product is added into a cellulase solution for enzymatic hydrolysis reaction to obtain reducing sugar. The method of the invention has the characteristics of mild process conditions, simple operation, high conversion rate and the like, and can be used for preparing reducing sugars such as glucose and xylose.

Description

technical field [0001] The invention belongs to the field of biomass utilization, and particularly relates to a method for improving lignocellulose enzymatic hydrolysis and saccharification efficiency by assisting ball milling with glucose and an application thereof. Background technique [0002] Due to the excessive use of petrochemical resources by humans, it has caused a series of environmental problems while causing the depletion of global petrochemical resources. common goals of workers. Among many new energy sources, biomass is regarded as an ideal material to replace petrochemical resources due to its advantages such as wide sources, low price, and environmental friendliness. Among them, the use of lignocellulose as raw material to produce clean fuel—ethanol has attracted more and more attention. However, due to the compact structure of lignocellulosic raw materials, they are highly resistant to enzymes, resulting in low enzymatic saccharification efficiency. There...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/02C12P19/14
CPCC12P19/02C12P19/14C12P2201/00
Inventor 李慧玲郑标黄嘉炜吴蔼民
Owner SOUTH CHINA AGRI UNIV