Microbial immobilization method for self-repairing of concrete cracks

A technology of microorganisms and concrete, which is applied in the interdisciplinary field of microbiology and civil engineering materials, to achieve the effects of enhancing sulfate erosion resistance, reducing compressive strength loss, and enhancing durability

Active Publication Date: 2020-05-12
ZHEJIANG OCEAN UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, microbial self-healing technology often uses porous carriers to immobilize microorganisms and then mix them into fresh concrete. The attached carriers include silica gel, nano-graphite sheets, ceramsite, and zeolite, etc. In terms of load capacity and compatibility between the carrier and the concrete matrix, the above-mentioned carriers have their own inherent shortcomings.

Method used

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  • Microbial immobilization method for self-repairing of concrete cracks
  • Microbial immobilization method for self-repairing of concrete cracks
  • Microbial immobilization method for self-repairing of concrete cracks

Examples

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Effect test

Embodiment 1

[0036] A method for immobilizing microorganisms, the specific steps of which are as follows:

[0037] (1) After activating Bacillus colii, inoculate it into a liquid medium, then cultivate it for 30 hours at a temperature of 35° C. to obtain a bacterial liquid, and then centrifuge the obtained bacterial liquid with a centrifuge at a speed of 5000 r / min for 25 min to obtain Bacterial slime, the composition of the above liquid medium is: ultrapure water 1L, yeast powder 5g, trimethylglycine 4.5g, tryptone 10g, ammonium sulfate 5g, glutamic acid 2g, urea 10g;

[0038] (2) Dilute the sludge with sterilized distilled water to a concentration of 6.5×10 7 cells / mL, make a bacterial suspension, crush the carrier coral reef calcareous sand to a particle size of 3mm, then put it into the bacterial suspension, and then soak the bacterial suspension at -0.08MPa pressure for 24 hours, then take out the coral reef calcium sand, and then dried to a constant weight in an environment of 45°C ...

Embodiment 2

[0042] A microbial immobilization method for concrete crack self-repair, the specific steps are as follows:

[0043] (1) Put cement, natural stone, recycled coarse aggregate, sand, silica fume, calcium chloride, polypropylene fiber, water reducing agent and water into the mixer, and stir evenly;

[0044] (2) Then put the microorganism-immobilized carrier prepared in Example 1 into it, and then stir for 1min, put the mixed concrete into the mould, and naturally maintain it to obtain the concrete with self-healing cracks, the above-mentioned microorganism-immobilized carrier The weight ratio in concrete raw materials is 10%.

Embodiment 3

[0046] A method for immobilizing microorganisms, the only difference between this embodiment and Example 1 is that the slurry used in step (3) also contains 0.03wt% phenylacetic acid-2-methylpropyl ester and 0.05wt% oxidized Styrene, the two are adsorbed into the carrier, due to its hydrophobicity, the arrangement of the molecules of the wrapping material changes, and the hydrophobic group is arranged on the outermost side, which makes the hydrophilicity of the carrier significantly reduced and the hydrophobicity increased, thereby avoiding bacteria or spores It can be revived in advance when it encounters water in concrete, and the dormant period will not be affected by water seepage. In addition, it can also participate in the deposition of calcium carbonate after the carrier is broken, and enhance the impermeability of the cracks, thereby reducing the compressive strength of the repaired concrete under freeze-thaw conditions. Reduction can enhance the frost resistance of the...

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Abstract

The invention provides a microbial immobilization method for self-repairing of concrete cracks and belongs to the field of microbiology and civil engineering material crossing technologies. The methodcomprises the following steps: immobilizing microorganisms or microbial spores in a carrier, and carrying out an immobilization process of wrapping treatment; carrying out a secondary immobilizationprocess of loading nutrient substances in the carrier; and carrying out an embedding process of doping the immobilized carrier obtained by the secondary immobilization process into a concrete raw material, wherein the carrier is coral reef calcareous sand; and the nutrient substances comprise 3, 5-dinitrobenzyl alcohol and sodium trimetaphosphate. According to the method provided by the invention,the survival rate of microorganisms can be increased; the crack repairing width can be increased (the maximum repairing width reaches 1 mm or above, and the average repairing width reaches 0.7-0.9 mm), and the concrete compressive strength is recovered by 80% or above; the compressive strength loss of the repaired concrete under sulfate erosion can be effectively reduced, the sulfate erosion resistance of the repaired concrete is improved, and the durability of the concrete is improved.

Description

technical field [0001] The invention belongs to the cross-technical field of microbiology and civil engineering materials, and in particular relates to a microbial immobilization method for self-repairing of concrete cracks. Background technique [0002] Studies have shown that the failure of concrete structures at home and abroad in the past 50 years has been caused by concrete cracks. The existence of concrete cracks makes the corrosive medium invade the interior of the concrete faster, causing corrosion of steel bars and damage to concrete structures. Concrete cracks are mainly caused by deformation (structure due to temperature and humidity changes, shrinkage creep, expansion, uneven subsidence, etc.) and direct stress of external loads (dynamic and static loads); statistical analysis of cracks in concrete due to deformation It accounts for about 80% of the total number of cracks, and the cracks caused by the direct stress of external loads account for about 20%. With t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C04B24/12C04B28/02C04B111/34
CPCC04B28/02C04B40/0039C04B2111/343C04B2201/50C04B2103/0001C04B14/28C04B14/106C04B22/00C04B24/126C04B24/00C04B24/04C04B24/12C04B22/16C04B14/02C04B14/06C04B16/0633C04B18/146C04B18/0427C04B22/124C04B2103/302C04B24/045C04B24/10
Inventor 李涛赖小颖黄亚娥孙浩铭张邦余昂烨陈晓伟王科英文有华
Owner ZHEJIANG OCEAN UNIV
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