Magnetic copper (II) chelating ferroferric oxide @carbon nanoparticles and preparation method thereof, and method for immobilizing laccase

A technology of ferric tetroxide and carbon nanoparticles, which is applied in biochemical equipment and methods, oxidoreductase, immobilized on or in inorganic carriers, etc. problems such as low volume, to achieve the effect of high yield, easy recycling, and large quantity

Pending Publication Date: 2020-05-12
ANHUI POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the magnetic Fe 3 o 4 , Fe 3 o 4 @SiO 2 , Fe 3 o 4 @MoS 2 Literature reports on nanoparticle immobilized laccase by @PEI and others (Chem.Eng.J.295(2016)201-206; ACS Appl.Mater.Interfaces3(2011)237-244; Chem.Eng.J.375(2019) 121947), but the current literature reports that the immobilization capacity of magnetic nanoparticles immobilized laccase is low, and the laccase loses part of its catalytic activity during the immobilization process, which cannot meet the needs of industrial production

Method used

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  • Magnetic copper (II) chelating ferroferric oxide @carbon nanoparticles and preparation method thereof, and method for immobilizing laccase
  • Magnetic copper (II) chelating ferroferric oxide @carbon nanoparticles and preparation method thereof, and method for immobilizing laccase
  • Magnetic copper (II) chelating ferroferric oxide @carbon nanoparticles and preparation method thereof, and method for immobilizing laccase

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Embodiment 1

[0058] A preparation method of magnetic copper (II) chelated ferric oxide@carbon nanoparticles, comprising the following steps:

[0059] 1) Preparation of hydroxyl / carboxyl functionalized magnetic Fe 3 o 4 The operation process of @C nanoparticles is as follows:

[0060] 1-1) In a three-necked flask, dissolve 0.4mmol of ferrous chloride and 0.8mmol of ferric chloride in 50mL of water, and add 4mL of concentrated ammonia (28% concentration by mass) under stirring in a nitrogen atmosphere. Add ammonia water 3 times; first add 2mL concentrated ammonia water, react at 60°C for 20min, then add 1mL concentrated ammonia water at 60°C, react at 60°C for 20min, then add 1mL concentrated ammonia water, ℃ for 20 minutes, and finally heated up to 90 ℃ for 1 hour to prepare Fe 3 o 4 Nanoparticle suspension, cooled to room temperature;

[0061] 1-2) To the above Fe 3 o 4 After adding 9mmol glucose and 180mmol sodium hydroxide to the nanoparticle suspension, transfer it to the autocla...

Embodiment 2

[0072] A preparation method of magnetic copper (II) chelated ferric oxide@carbon nanoparticles, comprising the following steps:

[0073] 1) Preparation of hydroxyl / carboxyl functionalized magnetic Fe 3 o 4 The operation process of @C nanoparticles is as follows:

[0074] 1-1) In a three-necked flask, dissolve 3mmol of ferrous chloride and 3mmol of ferric chloride in 100mL of water, and add 4mL of concentrated ammonia water (28% by mass concentration) under stirring in a nitrogen atmosphere, and 4mL of concentrated ammonia water Add 3 times; first add 2mL concentrated ammonia water, react at 70°C for 20min, then add 1mL concentrated ammonia water at 70°C, react at 70°C for 20min, then add 1mL concentrated ammonia water, The reaction was carried out at 100°C for 80 minutes, and the temperature was raised to 100°C for 2 hours to prepare Fe 3 o 4 Nanoparticle suspension, cooled to room temperature;

[0075] 1-2) To the above Fe 3 o 4 After adding 12mmol sucrose and 180mmol ...

Embodiment 3

[0083] A preparation method of magnetic copper (II) chelated ferric oxide@carbon nanoparticles, comprising the following steps:

[0084] 1) Preparation of hydroxyl / carboxyl functionalized magnetic Fe 3 o 4 The operation process of @C nanoparticles is as follows:

[0085] 1-1) In a three-necked flask, dissolve 0.4mmol of ferrous chloride and 0.8mmol of ferric chloride in 50mL of water, and add 4mL of concentrated ammonia (28% concentration by mass) under stirring in a nitrogen atmosphere. Add ammonia water 3 times; first add 2mL concentrated ammonia water, react at 60°C for 20min, then add 1mL concentrated ammonia water at 60°C, react at 60°C for 20min, then add 1mL concentrated ammonia water, ℃ for 20 minutes, and finally heated up to 90 ℃ for 1 hour to prepare Fe 3 o 4 Nanoparticle suspension, cooled to room temperature;

[0086] 1-2) To the above Fe 3 o 4 After adding 9mmol glucose and 180mmol sodium hydroxide to the nanoparticle suspension, transfer it to the autocla...

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Abstract

The invention provides magnetic copper (II) chelating ferroferric oxide @carbon nanoparticles and a preparation method thereof, and a method for immobilizing laccase. The preparation method comprisesthe steps of firstly preparing hydroxyl / carboxyl functionalized magnetic Fe3O4@C nanoparticles by a hydrothermal method, then performing coordination adsorption on Cu2+ ions to obtain magnetic Cu(II)chelating Fe3O4@C nanoparticles, and finally through the coordination effects of laccase histidine residues and Cu(II), preparing magnetic Cu(II) chelating Fe3O4@C nanoparticle immobilized laccase which is high in stability, high in activity and reusable. Compared with the prior art, the prepared magnetic Cu(II) chelating Fe3O4@C nanoparticle immobilized laccase is simple to operate, economic andenvironmentally-friendly; and the enzyme loading quantity is high, immobilized laccase is high in catalytic activity and high in stability, can be simply cycled and reused, and has great application prospects, and actual production cost can be effectively reduced.

Description

technical field [0001] The invention belongs to the field of preparation methods of immobilized laccase, in particular to magnetic Cu(II) chelated Fe 3 o 4 @C nanoparticle and its preparation method, the method for immobilizing laccase. Background technique [0002] Laccase is a kind of copper-containing polyphenol oxidase that is widely used. It can catalyze the oxidation of various phenolic dyes, substituted phenols, aromatic amines and other easily oxidized compounds. It has important applications in the treatment of phenolic wastewater and industrial papermaking wastewater. value. Although free laccase can efficiently catalyze under normal temperature and pressure and mild conditions, laccase is a protein composed of amino acids, and its molecular structure is very sensitive to the external environment. Changes can easily cause changes in the molecular structure of the enzyme protein, resulting in the loss of catalytic activity of the laccase. In addition, laccase is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N9/02
CPCC12N11/14C12N9/0061C12Y110/03002
Inventor 陈志明李志国汪春梅
Owner ANHUI POLYTECHNIC UNIV
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