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Separation process and application of natural free radical scavengers from Saxifrage tangutica

A Tanggut tiger ear and free radical technology is applied in the separation/purification, application, and medical preparations containing active ingredients of carboxylic acid compounds, which can solve the problem of natural free radical scavengers that have not been reported in literature and have not been systematic Chemical research and other issues, to achieve the effect of suitable for large-scale production, easy batch preparation, and low cost

Active Publication Date: 2021-06-22
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are no literature reports on the separation, preparation and application of natural free radical scavengers in Saxifrage tangutica, and the existing research has not been systematic.

Method used

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  • Separation process and application of natural free radical scavengers from Saxifrage tangutica
  • Separation process and application of natural free radical scavengers from Saxifrage tangutica
  • Separation process and application of natural free radical scavengers from Saxifrage tangutica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The separation process of natural free radical cleavage in Donut Tiger ear grass includes the following steps:

[0054] Step 1, extract: Take 500g Tang Gute Tiger ear grass is dry, roughly extract 1 g: 5 ml of methanol extraction, 4 times at room temperature, 2 h, filtrate, combined filtrate, ie filtrate A The amount of the filtrate A is in the amount of polyamide: Donut Tiger earwebed material = 1: 5 mixed and dried under reduced pressure, where the reduced pressure dry condition is a vacuum 50 mbar, temperature 40 ° C, ie Donut Tiger Purvant extract sample sample 160.2g;

[0055] Step 2, the microporous resin column coarse: Tang Gute tiger earbed extract sample sample, the sample is separated from the medium pressure chromatography column of the microporous resin, and the detection wavelength is 254 nm detection, collecting preparation The first major chromatographic fraction in the chromatogram figure 1 As shown, the distillate is obtained by drying the target component,...

Embodiment 2

[0062] The separation process of natural free radical cleavage in Donut Tiger ear grass includes the following steps:

[0063] Step 1, extract: Take 1000G Tang Gute Tiger ear grass is dry, roughly crushed, the liquid is extracted from 1 g: 100 ml of methanol, extract 2 times at room temperature, 4 h, filtrate, combined filtrate, ie filtrate A The amount of the filtrate A is in the amount of polyamide: the amount of Donut Tiger earned herbs = 1: 5 mixed and dried under reduced pressure, where the reduced pressure dry condition is a vacuum 250 mbar, temperature 60 ° C, ie Tang Guthu Earweled extract sample sample 719.3g;

[0064]Step 2, the microporous resin column coarse: Tang Gute tiger earbed extract sample sample, the sample is separated from the medium pressure chromatography column of the microporous resin, and the detection wavelength is 254 nm detection, collecting preparation The first primary chromatographic fraction in the chromatogram, the distillate was dried to dryness...

Embodiment 3

[0071] The separation process of natural free radical cleavage in Donut Tiger ear grass includes the following steps:

[0072] Step 1, extract: Take 1200g Tang Gute Tiger ear grass is dry, roughly extract 1 g: 100 mL of methanol extraction, extract 2 times at room temperature, 4 h, filtrate, combined filtrate, ie filtrate A The amount of the filtrate A is in the amount of polyamide: Donut Tiger earwebed material = 1: 5 mixed and dried under reduced pressure, where the reduced pressure dried conditions are vacuum 150 mbar, temperature 50 ° C, that is, Tang Guthu Earweled extracts sample sample 863.7g;

[0073] Step 2, the microporous resin column coarse: Tang Gute tiger earbed extract sample sample, the sample is separated from the medium pressure chromatography column of the microporous resin, and the detection wavelength is 254 nm detection, collecting preparation The first primary chromatographic fraction in the chromatogram, the distillate was discharged to the target component...

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Abstract

The invention relates to the technical field of separation of natural free radical scavenger in saxifrage tangutica, in particular to a separation process and application of the natural free radical scavenger in saxifrage tangutica. Its preparation method: extraction, microporous resin column rough separation, online free radical scavenger component screening, reversed-phase preparative column preparation, reversed-phase preparative liquid chromatography purification of Fr1-1 and hydrophilic / reversed-phase two of Fr1-3 Six steps of liquid chromatography purification. The present invention has low cost and product purity greater than 95%; the technical means adopted in the present invention can carry out large-scale production: raw material requirements are not high, the cost is low, and it is easy to prepare materials in batches; methanol is extracted by cold soaking at room temperature, and it is easy to operate; the separation adopts a microporous resin column For coarse separation, the microporous resin separation material can be installed in a medium-pressure column chromatography system, which is easy to scale; the reversed-phase preparative liquid chromatography or hydrophilic preparative liquid chromatography used in separation and purification is a fast isocratic method.

Description

Technical field [0001] The present invention relates to the field of natural free radical separation of natural free radicals in Donut Tiger ear, and in particular to the separation process of natural free radical densotrion in Donut Tiger ear. Background technique [0002] Tonalt tiger ear grass ( Saxifraga tangutica eNGL. ), Alias ​​Ganqing Tiger Earra, is Tiger Ear Grass ( Saxifragaceae ) 耳耳 (( Saxifraga The year, III, almost green herb, Tibetan medicine name: "Samiti", Chinese medicine called "Yuri", mainly distributed in Qinghai, Gansu, Tibet, Sichuan and the Bhutan and Kashmir region 2900 ~ 4900 meters above sea level Coniferous forest pressures. Tang Guthu earlast is commonly used in Tibetan medicine, but also into the medicine, "Chinese medicine resignation" records: its sexy taste is slightly bitter, Xin, cold. Main effects: clear liver gallbladder, spleen and stomach. Indications: hepatitis, cholecystitis, epidemic cold. Modern pharmacological studies have proved phenol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C51/47C07C65/03C07C67/56C07C69/84C07H15/203C07H1/08A61K31/192A61K31/235A61K31/7034A61P39/06A23L33/105
CPCA23V2002/00A61K31/192A61K31/235A61K31/7034A23L33/105A61P39/06C07C51/47C07C67/56C07H1/08C07H15/203C07C2601/16C07C65/03C07C69/84A23V2200/30
Inventor 党军陶燕铎王启兰邵赟
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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