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Ultrahigh-pressure extraction method of lentinan

A technology of lentinan and ultra-high pressure, which is applied in the purification of lentinan and the extraction of lentinan from lentinan by ultra-high pressure technology, can solve the problems of low extraction purity and low extraction rate, and achieve good thermal stability, large adsorption capacity, physical and chemical stable effect

Inactive Publication Date: 2020-05-22
南京萌源康德生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of conventional techniques such as relatively low extraction rate and low extraction purity, and provide a method for extracting lentinan from shiitake mushrooms using ultra-high pressure technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for extracting lentinan from shiitake mushrooms using ultra-high pressure technology, the steps are as follows:

[0021] (1) After the dried shiitake mushrooms are crushed through a 60-mesh sieve, put them into a pressure-resistant bag, add 10 times the volume of ethanol with a concentration of 70%, and process at an ultra-high pressure of 200MPA, 10min, and 18°C, reclaim the ethanol, and use it at 60 ℃ vacuum drying to obtain the crude product;

[0022] (2) Add appropriate amount of 75% ethanol to dissolve the obtained Lentinan crude product powder, adjust its pH value to 5.5, heat to 60°C, add 2.0% pectinase (1 million u / ml), mix well and keep enzymatic hydrolysis for 0.5h . Cool to room temperature, let stand, filter, discard the filter residue, and concentrate the filtrate under reduced pressure to obtain the crude extract of lentinan;

[0023] (3) Adsorb the obtained Lentinan crude extract with LX-100 type macroporous adsorption resin;

[0024] (4) Elut...

Embodiment example 2

[0029] A method for extracting lentinan from shiitake mushrooms using ultra-high pressure technology, the steps are as follows:

[0030] (1) After the dried shiitake mushrooms are crushed through an 80-mesh sieve, put them into a pressure-resistant bag, add 12 times the volume of ethanol with a concentration of 90%, and process at an ultra-high pressure of 300MPA, 15min, and 20°C, reclaim the ethanol at 60 ℃ vacuum drying to obtain the crude product;

[0031] (2) Add an appropriate amount of 75% ethanol to dissolve the obtained Lentinan crude product powder, adjust its pH value to 7.5, heat to 60°C, add 2.0% pectinase (1 million u / ml), mix well and keep enzymatic hydrolysis for 1.5h . Cool to room temperature, let stand, filter, discard the filter residue, and concentrate the filtrate under reduced pressure to obtain the crude extract of lentinan;

[0032] (3) Adsorb the obtained Lentinan crude extract with AB-8H macroporous adsorption resin;

[0033] (4) Elute the macropor...

Embodiment example 3

[0038] A method for extracting lentinan from shiitake mushrooms using ultra-high pressure technology, the steps are as follows:

[0039] (1) After the dried shiitake mushrooms are crushed into an 80-mesh sieve, put them into a pressure-resistant bag, add 12 times the volume of ethanol with a concentration of 80%, and process them under the conditions of ultra-high pressure 200MPA, 12min, and 18°C, recycle the ethanol, and heat it at 60°C The crude product was obtained by vacuum drying;

[0040] (2) Add an appropriate amount of 75% ethanol to dissolve the obtained lentinan crude product powder, adjust its pH value to 4.5, heat to 50°C, add 1.5% pectinase (1 million u / ml), mix well and maintain enzymatic hydrolysis for 2 hours. Cool to room temperature, let stand, filter, discard the filter residue, and concentrate the filtrate under reduced pressure to obtain the crude extract of lentinan;

[0041] (3) Adsorb the obtained Lentinan crude extract with H103 macroporous adsorption...

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Abstract

The invention relates to an extraction method of lentinan. According to the invention, an ultrahigh pressure technology is a treatment method for applying pressure of more than 100 MPa to raw materials under the conditions of normal temperature and near normal temperature, a solvent can enter plant cells under ultrahigh pressure, after pressure relief, the solvent can overflow along with effectivecomponents so as to achieve the high extraction efficiency of plant effective components, and lentinan is adsorbed and purified through a macroporous resin, so that the lentinan adsorption selectivity is good, adsorption and desorption are fast, the adsorption capacity is large, and the defects that in the conventional technology, the extraction rate is low, and the extraction purity is low are overcome.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry and relates to a method for purifying lentinan, in particular to a method for extracting lentinan from lentinan by ultra-high pressure technology. Background technique: [0002] Lentinusedodes (scientific name: Lentinusedodes), also known as mushroom, mushroom, northern mushroom, thick mushroom, thin mushroom, flower mushroom, shiitake, is a kind of edible fungus. The generally edible part is the fruiting body of shiitake mushrooms, and fresh shiitake mushrooms are dehydrated into dried shiitake mushrooms, which are convenient for transportation and storage. The edible part of dried shiitake mushrooms accounts for 72%, and every 100g edible part contains 13g of water, 1.8g of fat, 54g of carbohydrates, 7.8g of crude fiber, 4.9g of ash, 124mg of calcium, 415mg of phosphorus, 25.3mg of iron, 10.07mg of vitamin B, and vitamin B21 .13mg, Niacin 18.9mg. Shiitake mushrooms are rich in lentina...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 王俊
Owner 南京萌源康德生物技术有限公司
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