Cultivation method of Zhijin dictyophora rubrovolvata

A technology of bamboo fungus and cultivation method, which is applied in cultivation, plant cultivation, mushroom cultivation, etc., can solve the problems of long occupation time, different strain quality, nutrient loss, etc., achieve pure strain, shorten cultivation time, and improve quality Excellent effect

Inactive Publication Date: 2020-05-26
贵州久源顺生态农业开发有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional production process is cumbersome, there are many varieties of medium materials, and the production process takes a long time, resulting in a large amount of nutrient loss, and the quality of the strains is different, which brings disadvantages to the production of high-quality Dictyophora spp.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for cultivating Dictyophora zhijinhongtuo, comprising the following steps:

[0025] S1 mother seed slant culture: put the medium prepared by potato, glucose, dihydrogen phosphate, vitamin b, agar and water into the test tube, sterilize it with a temperature of 124 degrees under high pressure, place it on a slope, and put it on a temperature of 26 degrees. Inoculate and cultivate for 10 days;

[0026] S2 liquid strain culture: put the strain after step S1 into the liquid culture medium prepared by bran, corn flour, soybean flour, sawdust and Juncao in the shake flask for 6 days;

[0027] S3 solid fine powder medium culture: Inoculate the strains after S2 culture into the solid fine powder medium prepared by miscellaneous wood chips, cotton hulls, corn flour, gypsum, dihydrogen phosphate, magnesium sulfate and carrot powder for 6 Tianfa bacteria;

[0028] S4 liquid strain culture: re-inoculate the strain after step S3 cultivation into the fermenter containing t...

Embodiment 2

[0037] A method for cultivating Dictyophora zhijinhongtuo, comprising the following steps:

[0038] S1 mother seed slant culture: put the medium prepared by potato, glucose, dihydrogen phosphate, vitamin b, agar and water into the test tube, sterilize it with a temperature of 124 degrees under high pressure, place it on a slope, and put it on a temperature of 26 degrees. inoculation, cultured for 11 days;

[0039] S2 liquid strain culture: the strains after the cultivation in step S1 were inserted into a shake flask for 7 days in a liquid medium prepared from bran, corn flour, soybean flour, sawdust and Juncao;

[0040] S3 solid fine powder medium culture: Inoculate the strains after S2 culture into the solid fine powder medium prepared by miscellaneous wood chips, cotton hulls, corn flour, gypsum, dihydrogen phosphate, magnesium sulfate and carrot powder for 7 Tianfa bacteria;

[0041] S4 liquid strain culture: re-inoculate the strain after step S3 cultivation into the ferm...

Embodiment 3

[0050] A method for cultivating Dictyophora zhijinhongtuo, comprising the following steps:

[0051] S1 mother seed slant culture: put the medium prepared by potato, glucose, dihydrogen phosphate, vitamin b, agar and water into the test tube, sterilize it with a temperature of 124 degrees under high pressure, place it on a slope, and put it on a temperature of 26 degrees. inoculation, and cultivated for 12 days;

[0052] S2 liquid strain culture: the strains after the cultivation in step S1 were inserted into the liquid culture medium prepared by bran, corn flour, soybean flour, sawdust and Juncao in shake flasks for 8 days;

[0053] S3 solid fine powder medium culture: Inoculate the strains after S2 culture into solid fine powder medium prepared by miscellaneous wood chips, cotton hulls, corn flour, gypsum, dihydrogen phosphate, magnesium sulfate and carrot powder for 8 Tianfa bacteria;

[0054] S4 liquid strain culture: re-inoculate the strain after step S3 cultivation into t...

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PUM

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Abstract

The invention discloses a cultivation method of Zhijin dictyophora rubrovolvata and relates to the technical field of cultivation to solve the problems that conventional processes are tedious in preparation and take longer preparation time. The cultivation method comprises steps as follows: S1, slant culture of a mother strain; S2, culture of a liquid strain; S3, culture in a solid-state fine powder culture medium; S4, culture of the liquid strain; S5, bamboo fungus bagging; and S6, net pavement and soil replacement. A liquid-solid-liquid coupling technology is adopted, liquid fermentation andsolid-state fermentation are connected in series in terms of process according to production demands, a new technological procedure is formed, the strain culture time is shortened, and the strain ispure and high in quality.

Description

technical field [0001] The invention relates to the field of cultivation technology, in particular to a method for cultivating Dictyophora zhijinhongtuo. Background technique [0002] The artificial cultivation of red-tuo bamboo fungus in China originated in Zhijin County, Guizhou Province; Zhijin County began to plant wild bamboo fungus in 1983, and Hongtuo bamboo fungus was successfully cultivated in 1986. In the early 1990s, 32 townships in the county were planted with bamboo fungus , with an area of ​​nearly ten thousand mu. At present, Guizhou has formed a bamboo fungus industry with Zhijin County as the distribution center and Bijie City, Anshun City, and Qianxinan Prefecture as the main production areas. At present, the production and cultivation mode of the red-tuo bamboo fungus is still the model in the 1980s. The cycle is long and the output is unstable, which greatly restricts the development of Guizhou Zhijin red-tuo bamboo fungus industry. The traditional proce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20A01G18/40
CPCA01G18/00A01G18/20A01G18/40
Inventor 刘德育丁远江丁统彪
Owner 贵州久源顺生态农业开发有限责任公司
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