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Preparation method of boar semen diluent

A diluent, pig semen technology, applied in the field of animal husbandry, can solve the problems of pig semen failure, short storage time, etc., and achieve the effect of increasing the storage time and reducing the use cost.

Inactive Publication Date: 2020-05-29
厦门汇承药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the present invention provides a preparation method of a pig semen diluent, which solves the problem that the existing diluents have a short storage time during pig semen preservation, and it is easy to make the pig semen lose efficacy. There are certain deficiencies

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0025] The embodiment of the present invention provides a preparation method of a pig semen diluent, wherein the pig semen diluent comprises the following components in parts by weight: 30 parts of nutrients, 15 parts of buffer, 1 part of potassium chloride, 1 part of ethylenediaminetetraacetic acid, protective 1 part of agent, 0.1 part of vitamin E, 2 parts of enrofloxacin, 0.5 part of amphotericin B, and 1000 parts of solution.

[0026] Wherein the nutrient is one of glucose, fructose, lactose and mannose, preferably glucose.

[0027] The buffer solution is a mixture of sodium dihydrogen phosphate and disodium hydrogen phosphate, and the ratio of sodium dihydrogen phosphate to disodium hydrogen phosphate is 1:1.

[0028] The protective agent is a mixture of bovine serum albumin and glycerin, wherein the ratio of bovine serum albumin and glycerin is 1:3-4.

[0029] Wherein the solution is one of distilled water, distilled water and triple distilled water.

[0030] A prepara...

Embodiment 2

[0038] The embodiment of the present invention provides a preparation method of a pig semen diluent, wherein the pig semen diluent comprises the following components in parts by weight: 35 parts of nutrients, 20 parts of buffer, 2 parts of potassium chloride, 1.5 parts of ethylenediaminetetraacetic acid, protective 2 parts of medicine, 0.2 parts of vitamin E, 3 parts of enrofloxacin, 0.6 parts of amphotericin B, and 1200 parts of solution.

[0039] Wherein the nutrient is one of glucose, fructose, lactose and mannose, preferably glucose.

[0040] The buffer solution is a mixture of sodium dihydrogen phosphate and disodium hydrogen phosphate, and the ratio of sodium dihydrogen phosphate to disodium hydrogen phosphate is 1:1.

[0041] The protective agent is a mixture of bovine serum albumin and glycerin, wherein the ratio of bovine serum albumin and glycerin is 1:3-4.

[0042] Wherein the solution is one of distilled water, distilled water and triple distilled water.

[0043]...

Embodiment 3

[0051] The embodiment of the present invention provides a preparation method of a pig semen diluent, wherein the pig semen diluent comprises the following components in parts by weight: 40 parts of nutrients, 25 parts of buffer, 3 parts of potassium chloride, 2 parts of ethylenediaminetetraacetic acid, protective 13 parts of medicine, 0.3 parts of vitamin E, 5 parts of enrofloxacin, 0.8 parts of amphotericin B, and 1400 parts of solution.

[0052] Wherein the nutrient is one of glucose, fructose, lactose and mannose, preferably glucose.

[0053] The buffer solution is a mixture of sodium dihydrogen phosphate and disodium hydrogen phosphate, and the ratio of sodium dihydrogen phosphate to disodium hydrogen phosphate is 1:1.

[0054] The protective agent is a mixture of bovine serum albumin and glycerin, wherein the ratio of bovine serum albumin and glycerin is 1:3-4.

[0055] Wherein the solution is one of distilled water, distilled water and triple distilled water.

[0056] ...

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Abstract

The invention provides a preparation method of a boar semen diluent, and relates to the field of livestock breeding. The boar semen diluent is prepared from the following components in parts by weight: 30-40 parts of nutrients, 15-25 parts of a buffer solution, 1-3 parts of potassium chloride, 1-2 parts of ethylene diamine tetraacetic acid, 1-3 parts of a protective agent, 0.1-0.3 part of vitaminE, 2-5 parts of enrofloxacin, 0.5-0.8 part of amphotericin B and 1000-1400 parts of a solution, wherein the nutrient is one of glucose, fructose, lactose and mannose, preferably glucose, the buffer solution is a mixture of sodium dihydrogen phosphate and disodium hydrogen phosphate, the ratio of sodium dihydrogen phosphate to disodium hydrogen phosphate is 1: 1, the protective agent is a mixture of bovine serum albumin and glycerin, and the ratio of bovine serum albumin to glycerin is 1: (3-4). Through reasonable material selection and preparation mode, a same proper preservation environment can be provided for boar semen, so that the diluent can greatly prolong the preservation time of boar semen, and the later insemination operation is facilitated.

Description

technical field [0001] The invention relates to the technical field of animal husbandry, in particular to a preparation method of a pig semen diluent. Background technique [0002] Nowadays, animal husbandry has become an important industry. With the continuous development and progress of society, people have started to breed scientifically. In order to optimize and improve the breed of pigs in breeding, the semen of boars has appeared to be transported This method is also called artificial insemination. This technology has greatly promoted the development of my country's animal husbandry industry and made a great contribution to the improvement of pig breeds. . [0003] For now, diluents are needed for artificial insemination between pig semen. However, most of the diluents are in powder form, and some liquid diluents are used. However, these diluents Agent, when carrying out the preservation of pig semen, the time of preservation is shorter, makes pig semen invalidation ea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0221A01N1/0226
Inventor 刘凯卫潘英萍
Owner 厦门汇承药业有限公司
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