Antibacterial fermentation additive based on covalent organic framework material and preparation and application thereof

A covalent organic framework and additive technology, applied in the fields of microbiology, material chemistry and biology, can solve problems such as bacterial contamination, achieve the effects of improving stability, avoiding economic losses, and improving production efficiency

Active Publication Date: 2020-05-29
NANKAI UNIV
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  • Abstract
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Problems solved by technology

[0006] The present invention aims to provide an antibacterial fermentation additive based on covalent organic framework materials to solve the problem of bacterial contamination in the fermentation process in the field of biological manufacturing. This technology is simple, efficient, and low in cost. At the same time, it can also significantly improve the stability of biomolecules, which can effectively reduce its cost and avoid economic losses.

Method used

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  • Antibacterial fermentation additive based on covalent organic framework material and preparation and application thereof
  • Antibacterial fermentation additive based on covalent organic framework material and preparation and application thereof
  • Antibacterial fermentation additive based on covalent organic framework material and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: The synthesis of covalent organic framework materials TPA-DMTP, TPB-DMTP, TPT-DMTP used for loading, the specific implementation steps are as follows:

[0030] TPA-DMTP: Weigh the ligands tris(4-aminophenyl)amine (TPA 11.6mg) and 2,5-dimethoxybenzene-1,4-dicarbaldehyde (DMTP 11.7mg) and add them to thick-walled heat-resistant Add 0.2mL o-dichlorobenzene, 0.8mL n-butanol and 0.2mL, 3M acetic acid to the glass tube, then quickly freeze in liquid nitrogen, then vacuumize, and then seal the tube with a flame of an oxygen hydrogen machine. Put the sealed glass tube in an oven at 120°C for 3 days.

[0031] TPB-DMTP: Weigh the ligands 1,3,5-tris(4-nitrophenyl)benzene (TPB 14,1 mg) and 2,5-dimethoxybenzene-1,4-dicarbaldehyde (DMTP 11.7mg) into a thick-walled heat-resistant glass tube, then add 0.6mL o-dichlorobenzene, 0.6mL n-butanol and 0.25mL, 3M acetic acid, then quickly freeze in liquid nitrogen, then vacuumize, and then use a hydrogen oxygen machine flame S...

Embodiment 2

[0035] Example 2: Adsorption of covalent organic framework materials for nisin

[0036] Weigh 10 mg of COFs material and traditional inorganic material SBA-15, add 5 mL of nisin buffer solution containing 0.5 mg / mL directly, shake on a shaker at 37 ° C, take supernatant at regular intervals, and use BCA protein quantification method to quantify The amount of nisin contained in the supernatant was used to calculate the amount of nisin adsorbed by the material, and the final adsorption amount is shown in Table 1.

[0037] Table 1 Adsorption capacity of materials

[0038]

Embodiment 3

[0039]Embodiment 3: MIC antibacterial test

[0040] Bacterial solution preparation: Pick a single colony of Lactobacillus brevis from the plate and inoculate it into MRS liquid medium, culture at 37°C for 10 hours, and count. Preparation of the porous frame material suspension: weigh a certain amount of the porous frame material compound, put it on a ultra-clean workbench and irradiate it with an ultraviolet lamp for 30 minutes. Prepare several sterile test tubes and mark them. Use the MRS liquid medium to prepare the material into a series of suspensions with different concentrations, place them in centrifuge tubes, and inoculate Lactobacillus brevis so that the concentration of the suspension is 1×10 7 ~2×10 7 CFU / mL (CFU / mL: total number of microbial communities per milliliter of liquid). The control group was sterile liquid medium, liquid medium after inoculation and material suspension without inoculation. Put it on a shaker at 37°C and 150rpm for cultivation, and add...

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Abstract

Aiming at the regulation and control problem in the fermentation process, a covalent organic framework material is creatively designed as a novel carrier material with wide applicability, and antibacterial molecules (such as protein, enzyme, polypeptide and antibiotics) are efficiently loaded through an adsorption means, so that the intelligent pH response industrial additive is prepared and is used for regulating and controlling infectious microbe pollution in the fermentation process. The novel fermentation additive prepared by the technology has high stability and durability, and successfully solves the problem of infectious microbe pollution in the fermentation process in the field of biological manufacturing.

Description

technical field [0001] The present invention relates to material chemistry, biology and microbiology, especially to an antibacterial fermentation additive based on a covalent organic framework material, its preparation method and its application in the field of biomanufacturing. Background technique [0002] Green biomanufacturing is of great significance to the sustainable development of the economy and society. Among them, the development of intelligent regulation can improve the fermentation efficiency of the medium, save labor costs, and effectively avoid economic losses, which is the current trend in the field of biomanufacturing. However, how to achieve fine regulation is one of the major difficulties, especially in the fermentation process, the fermentation liquid is rich in nutrients and is a good medium for the growth of microorganisms. Pollution can cause huge economic losses. [0003] To prevent and control harmful microorganisms during fermentation, synthetic an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/10A01N43/90A01P3/00A01P1/00C12C5/02
CPCA01N25/10A01N43/90C12C5/02
Inventor 陈瑶张振杰段文杰张赛男
Owner NANKAI UNIV
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