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Rice MIT2 gene and protein encoded by same, and application thereof

A rice and protein technology, applied in the field of genetic engineering, can solve the problems such as the reduction of tiller number and the loss of OsTB1 function, and achieve the effect of obvious screening effect, huge economic value and improving rice productivity.

Active Publication Date: 2020-06-02
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

found that overexpression of OsTB1 reduced tiller number, while in fc1 Increased tillering in mutants with loss of OsTB1 function

Method used

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  • Rice MIT2 gene and protein encoded by same, and application thereof
  • Rice MIT2 gene and protein encoded by same, and application thereof
  • Rice MIT2 gene and protein encoded by same, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Obtaining and Phenotype Analysis of Mutants

[0050] A japonica rice variety Nipponbare was chemically induced by EMS to obtain a mutant with moderately increased tillers Moderately Increased Tiller-2 ( mit2 ) and its allelic mutants mit2-1 . mit2 Mutations to base deletions, allelic mutants mit2-1 It has a similar dwarf multi-tiller phenotype, and the mutation is a base substitution.

[0051] Phenotypic analysis showed that the mutant had many changes compared with the wild type ( figure 1 ). rice mit2 The plant height of the mutant is shorter than that of the wild type, and the plant height at the mature stage is slightly shorter, about 60% of the wild type, and the internodes are shortened to a certain extent; mit2 The mutant had a moderate increase in tillering, which was about twice that of the wild type. mutant mit2 and its allelic mutants had fewer branches, lower fertility, smaller grains, and open glumes.

Embodiment 2

[0052] Example 2 Rice MIT2 Gene acquisition and functional complementation verification

[0053] 1. Rice MIT2 Gene acquisition

[0054] of the present invention MIT2 mutant mit2 cloned. homozygous mutant mit2 When crossed with Dular, an indica rice variety with normal phenotype and high polymorphism, all crosses F 1 The medium individuals showed normal plant height and tiller number. f 2 Obtain segregating populations for genetic analysis and gene mapping. The analysis of the lines with segregated traits in the F2 generation showed that the segregation ratio of the wild type and the mutant conformed to the 3:1 genetic segregation ratio, which indicated that the mutant trait was controlled by a pair of recessive genes.

[0055] In order to locate the position of the gene controlling the moderately increased tillering mutant, we used the F 2 The segregated population was used as the targeted population, and 10 strains of F 2 A DNA pool was constructed from a mutan...

Embodiment 3

[0063] Example 3 Rice MIT2 gene expression pattern

[0064] to be clear MIT2 The tissue expression pattern of the gene is to extract the RNA of different tissues (seeds, roots, stems, leaves, leaf sheaths, young spikes, flowers, tiller buds, seedlings, and young roots) of the japonica rice variety Nipponbare, reverse transcribe into cDNA, and use the rice Ubiquitin gene as As an internal reference, Real-time PCR was used to detect the expression level of the gene in various tissues of rice, and the results showed that MIT2 The gene is expressed in the above tissues of rice, with the highest expression level in young roots, followed by higher expression levels in young panicle seeds and seedlings, and also expression levels in leaf sheaths and tiller buds, and relatively lower levels in seeds and mature roots ( Figure 4 ).

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Abstract

The invention provides a rice MIT2 gene and a protein encoded by the rice MIT2 gene. The sequence of an encoding region of the rice MIT2 gene is shown as SEQ ID No. 2, and the sequence of the proteinencoded by the rice MIT2 gene is shown as SEQ ID No. 3. The gene is expressed in a plurality of tissues. Experiments prove that the MIT2 gene has the function of inhibiting rice tillering and thus allows rice plants to be heightened. The invention further provides a mutant gene of the MIT2 gene and an allelic mutant mit2-1 of the mutant gene. The mutant gene and the allelic mutant mit2-1 enable rice to be short in plant height, increased in tillering moderation, reduced in branch number and stem number, lowered in fertility, lessened in grain size and open in glumes. The rice MIT2 gene and themutants thereof provided by the invention play an important role in genetic improvement breeding of rice germplasm resources, and are expected to regulate and control the formation of rice plant types and the tiller number of rice plants, so directional designing of the plant types or the tiller number is realized to improve the productivity of rice.

Description

technical field [0001] The invention belongs to the field of genetic engineering, in particular, relates to a rice MIT2 Gene, its encoded protein and its application in inhibiting branching in rice. Background technique [0002] Rice ( Oryza sativa) Belonging to the Poaceae Oryza genus, it is one of the most important food crops. Rice tillering is a very important agronomic trait, and one effective tiller corresponds to one panicle. Tillering is a key factor in determining rice yield. Rice tillering is not only an important factor related to rice yield, but also a central topic related to plant architecture in biological discussions. Some genes and Quantitative Traits Location (QTL) related to tillering have been mapped out. Therefore, further excavating rice tillering regulatory genes and elucidating the tillering mechanism are of great significance for shaping rice plant architecture and improving rice yield. [0003] The rice tiller formation process is divided int...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8261
Inventor 李学勇常颖赵金凤谢婷婷房静静袁守江
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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