A kind of rice mit2 gene and its encoded protein and application

A rice and protein technology, applied in the field of genetic engineering, can solve the problems of OsTB1 function loss and tiller number reduction, achieve obvious screening effect, huge economic value, and improve rice productivity

Active Publication Date: 2020-07-24
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

found that overexpression of OsTB1 reduced tiller number, while in fc1 Increased tillering in mutants with loss of OsTB1 function

Method used

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  • A kind of rice mit2 gene and its encoded protein and application
  • A kind of rice mit2 gene and its encoded protein and application
  • A kind of rice mit2 gene and its encoded protein and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Obtaining and Phenotype Analysis of Mutants

[0050] A japonica rice variety Nipponbare was chemically induced by EMS to obtain a mutant with moderately increased tillers Moderately Increased Tiller-2 ( mit2 ) and its allelic mutants mit2-1 . mit2 Mutations to base deletions, allelic mutants mit2-1 It has a similar dwarf multi-tiller phenotype, and the mutation is a base substitution.

[0051] Phenotypic analysis showed that the mutant had many changes compared with the wild type ( figure 1 ). rice mit2 The plant height of the mutant is shorter than that of the wild type, and the plant height at the mature stage is slightly shorter, about 60% of the wild type, and the internodes are shortened to a certain extent; mit2 The mutant had a moderate increase in tillering, which was about twice that of the wild type. mutant mit2 and its allelic mutants had fewer branches, lower fertility, smaller grains, and open glumes.

Embodiment 2

[0052] Example 2 Rice MIT2 Gene acquisition and functional complementation verification

[0053] 1. Rice MIT2 Gene acquisition

[0054] of the present invention MIT2 mutant mit2 cloned. homozygous mutant mit2 When crossed with Dular, an indica rice variety with normal phenotype and high polymorphism, all crosses F 1 The medium individuals showed normal plant height and tiller number. f 2 Obtain segregating populations for genetic analysis and gene mapping. The analysis of the lines with segregated traits in the F2 generation showed that the segregation ratio of the wild type and the mutant conformed to the 3:1 genetic segregation ratio, which indicated that the mutant trait was controlled by a pair of recessive genes.

[0055] In order to locate the position of the gene controlling the moderately increased tillering mutant, we used the F 2 The segregated population was used as the targeted population, and 10 strains of F 2 A DNA pool was constructed from a mutant ...

Embodiment 3

[0063] Example 3 Rice MIT2 gene expression pattern

[0064] to be clear MIT2 The tissue expression pattern of the gene is to extract the RNA of different tissues (seeds, roots, stems, leaves, leaf sheaths, young spikes, flowers, tiller buds, seedlings, and young roots) of the japonica rice variety Nipponbare, reverse transcribe into cDNA, and use the rice Ubiquitin gene as As an internal reference, Real-time PCR was used to detect the expression level of the gene in various tissues of rice, and the results showed that MIT2 The gene is expressed in the above tissues of rice, with the highest expression level in young roots, followed by higher expression levels in young panicle seeds and seedlings, and also expression levels in leaf sheaths and tiller buds, and relatively lower levels in seeds and mature roots ( Figure 4 ).

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Abstract

The invention provides a rice MIT2 The gene and its encoded protein, its coding region sequence is shown in SEQ ID No.2, and its encoded protein sequence is shown in SEQ ID No.3. This gene is expressed in multiple tissues. Experiments have proved that MIT2 has the function of inhibiting rice tillering and making rice plants taller. The present invention also provides MIT2 Mutated genes and their allelic mutants mit2‑1 , They make the rice show the performance of shorter plant height, moderate increase in tillering, fewer branches, lower fertility, smaller grains, and open glumes. Rice provided by the invention MIT2 Genes and their mutants play an important role in the genetic improvement and breeding of rice germplasm resources. They are expected to regulate the formation of rice plant type and the number of tillers, and then design the plant type or number of tillers to improve rice productivity.

Description

technical field [0001] The invention belongs to the field of genetic engineering, in particular, relates to a rice MIT2 Gene, its encoded protein and its application in inhibiting branching in rice. Background technique [0002] Rice ( Oryza sativa) Belonging to the Poaceae Oryza genus, it is one of the most important food crops. Rice tillering is a very important agronomic trait, and one effective tiller corresponds to one panicle. Tillering is a key factor in determining rice yield. Rice tillering is not only an important factor related to rice yield, but also a central topic related to plant architecture in biological discussions. Some genes and Quantitative Traits Location (QTL) related to tillering have been mapped out. Therefore, further excavating rice tillering regulatory genes and elucidating the tillering mechanism are of great significance for shaping rice plant architecture and improving rice yield. [0003] The rice tiller formation process is divided int...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8261
Inventor 李学勇常颖赵金凤谢婷婷房静静袁守江
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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