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Method for generating methane by bioelectrochemistry reduction of CO2

A bioelectrochemical and methane-producing technology, applied in the field of CO2 resource utilization, can solve the problems of no direct and simple method, and achieve the effect of simple operation, strong specificity and low energy consumption

Active Publication Date: 2020-06-02
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no direct and simple method for the analysis of methanogenesis pathways in bioelectrochemical systems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Take a strain of Methanosarcina barkeri isolated from the samples of Shengli Oilfield as the object to be detected, add culture solution into the bioelectrolyte cell body and inoculate the methanogen, connect the electrolyzer to the electrochemical workstation, and detect the methanogen according to the following method The optimization conditions for:

[0042] (1) Extract the RNA of methanogens in the bioelectrochemical reaction system to be tested and the control system: establish an anaerobic bioelectrochemical reaction system inoculated with Methanosarcina barkeri culture solution, the inoculum size is 10%, the set potential is -0.8V, and the reaction is performed at room temperature , the control system was 0 V with hydrogen (only hydrogen-mediated indirect electron transfer pathway) and -0.35 V without hydrogen (only direct electron transfer pathway). After reacting for 60 hours, the methane production in the cell body of the reaction system was measured to be 1.3...

Embodiment 2

[0051] Take a strain of Methanosarcina barkeri isolated from the Jiangsu oilfield sample as the object to be detected, add culture solution to the cell body of the bioelectrolysis cell and inoculate the methanogen, connect the electrolysis cell to the electrochemical workstation, and detect methane production according to the following method The optimization conditions for:

[0052] (1) Extract the RNA of methanogens in the bioelectrochemical reaction system to be tested and the control system: establish an anaerobic bioelectrochemical reaction system inoculated with Methanosarcina barkeri culture solution, the inoculum size is 30%, the setting potential is -0.5V, and the reaction is performed at room temperature , the control system was 0 V with hydrogen (only hydrogen-mediated indirect electron transfer pathway) and -0.35 V without hydrogen (only direct electron transfer pathway). After reacting for 72 hours, the amount of methane produced in the cell body of the reaction s...

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Abstract

The invention relates to a method for generating methane by bioelectrochemistry reduction of CO2. The method comprises the following steps of (1) extracting a bioelectrochemistry reaction system to betested and RNA of bacteria generating methane in a control system; (2) performing reverse transcription on extracted RNA into cDNA; (3) performing fluorescent quantitation PCR on the obtained cDNA byrespectively adopting specific primers of functional genes ccdA and frhB; and (4) calculating the ratio of expression levels of two functional genes, comparing the control system, judging a methane generating way, and selecting a reaction condition corresponding to the way to generate the methane. Compared with the prior art, the method has the characteristics of being higher in specificity, moreconvenient to operate, more accurate to detect, higher in methane generating efficiency and the like.

Description

technical field [0001] The present invention relates to CO 2 The field of resource utilization, especially related to a method of reducing CO by bioelectrochemical 2 method of producing methane. Background technique [0002] CO 2 As one of the main greenhouse gases, its increase in the atmosphere may lead to global warming and increased climate extremes. How to effectively reduce CO in the atmosphere 2 emissions and converting them into high value-added chemicals has become a global research hotspot. Common transformation methods include chemical transformation and biological transformation. Chemical methods often require high temperature, high pressure and catalysts. In contrast, biotransformation can occur at room temperature and normal pressure, and the reaction conditions are mild, so it has broad application prospects. [0003] Using a bioelectrochemical system to convert CO 2 Reduction of methane is one of the current research hotspots. When only methanogens (s...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/6851C12Q1/04C12P5/02C12R1/01
CPCC12Q1/689C12Q1/6851C12P5/023C12Q2600/158C12Q2521/107C12Q2531/113C12Q2563/107Y02E50/30
Inventor 牟伯中周蕾刘金峰杨世忠白杨梁甜甜
Owner EAST CHINA UNIV OF SCI & TECH