Application of oryza sativa L. heavy metal transport protein OsHMA6 on aspect of lowering Cu poison

A heavy metal and rice technology, applied in the field of genetic engineering, can solve the problems of biomass reduction, plant toxicity, etc.

Pending Publication Date: 2020-06-05
AGRI GENOME INST OF SHENZHEN CHINESE ACADEMY OF AGRI SCI
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Likewise, excess copper is toxic to plants (Abdel-Ghany et al, 2005; Andrés-Colás et al, 2006) and causes plant roo

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of oryza sativa L. heavy metal transport protein OsHMA6 on aspect of lowering Cu poison
  • Application of oryza sativa L. heavy metal transport protein OsHMA6 on aspect of lowering Cu poison
  • Application of oryza sativa L. heavy metal transport protein OsHMA6 on aspect of lowering Cu poison

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] 1. Cloning of rice gene OsHMA6 sequence

[0019] 1) Extraction of total RNA

[0020] Rice (Wuyunjing No. 7) seedlings grow to the 3-leaf stage, treated with 0.2mM nitrate nitrogen for 6 hours, immediately take the roots and quickly put them in liquid nitrogen for cryopreservation, weigh about 0.1g of roots, and grind them with liquid nitrogen , grind fully and add to a 1.5ml centrifuge tube, quickly add 1ml Trizol reagent (purchased from Invitrogen, USA), shake well and oscillate, and extract total RNA.

[0021] 2) Cloning of the full-length CDS of the OsHMA6 gene

[0022] The CDS sequence of the rice OsHMA6 (Os02g0172600) gene was retrieved from the gene database of the rice database (Rice Annotation Project database, https: / / rapdb.dna.affrc.go.jp / index.html), and primers were designed with the software Primer 5.0 Sequence (below), the full-length CDS sequence of OsHMA6 was fished out from the tissue cDNA library.

[0023] P1:5'-ATGGCCCATCTCCAGCTCACGCCGC-3'(SEQ ID N...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses application of an oryza sativa L. heavy metal transport protein OsHMA6 and an encoding gene thereof on an aspect of improving Cu poison or Cu accumulation by a plant. The expression of the gene in engineering saccharomyces cerevisiae can improve the endurance capacity of the saccharomyces cerevisiae for Cu, and the content of Cu in the saccharomyces cerevisiae can be lowered so as to indicate that the OsHMA6 protein has a capacity of degrading heavy metal Cu poison. The gene has a potential for being applied to the genetic engineering breeding of the Cu poison resistance of the plant, the expression of the gene in the plant is regulated and controlled, the tolerance of a transgenic plant for the heavy metal Cu can be changed, and the accumulation of the heavy metalCu by the transgenic plant can be changed.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and relates to the application of rice heavy metal transporter OsHMA6 and its coded gene in reducing copper toxicity. Background technique [0002] Copper (Cu) is an essential micronutrient that plays a key role in many plant processes such as photosynthesis, oxidative stress, nitrogen and carbon metabolism, hormone sensing, and cell wall synthesis (Pilon et al, 2006; Hansch and Mendel, 2009). Copper deficiency results in stunted plant growth, yellowing of young leaves, and developmental defects (Klaumann et al, 2011). Copper deficiency also impairs pollen fertility and reduces growth rate, seed setting rate and yield (Huang et al, 2016). Copper also plays an important role in human health, and copper deficiency leads to anemia and immunodeficiency (Collins and Klevay, 2011; Gulec and Collins, 2014). Currently, more than 2 billion people worldwide suffer from micronutrient deficiencies such ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K14/415C12N15/81C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/81C12N15/8271
Inventor 陈景光叶国友
Owner AGRI GENOME INST OF SHENZHEN CHINESE ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap