Application of oryza sativa L. heavy metal transport protein OsHMA6 on aspect of lowering Cu poison

Abstract

The invention discloses application of an oryza sativa L. heavy metal transport protein OsHMA6 and an encoding gene thereof on an aspect of improving Cu poison or Cu accumulation by a plant. The expression of the gene in engineering saccharomyces cerevisiae can improve the endurance capacity of the saccharomyces cerevisiae for Cu, and the content of Cu in the saccharomyces cerevisiae can be lowered so as to indicate that the OsHMA6 protein has a capacity of degrading heavy metal Cu poison. The gene has a potential for being applied to the genetic engineering breeding of the Cu poison resistance of the plant, the expression of the gene in the plant is regulated and controlled, the tolerance of a transgenic plant for the heavy metal Cu can be changed, and the accumulation of the heavy metalCu by the transgenic plant can be changed.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and relates to the application of rice heavy metal transporter OsHMA6 and its coded gene in reducing copper toxicity. Background technique [0002] Copper (Cu) is an essential micronutrient that plays a key role in many plant processes such as photosynthesis, oxidative stress, nitrogen and carbon metabolism, hormone sensing, and cell wall synthesis (Pilon et al, 2006; Hansch and Mendel, 2009). Copper deficiency results in stunted plant growth, yellowing of young leaves, and developmental defects (Klaumann et al, 2011). Copper deficiency also impairs pollen fertility and reduces growth rate, seed setting rate and yield (Huang et al, 2016). Copper also plays an important role in human health, and copper deficiency leads to anemia and immunodeficiency (Collins and Klevay, 2011; Gulec and Collins, 2014). Currently, more than 2 billion people worldwide suffer from micronutrient deficiencies such ...

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Problems solved by technology

[0003] Likewise, excess copper is toxic to plants (Abdel-Ghany et al, 2005; Andrés-Colás et al, 2006) and causes plant root growth inhibition, reduced biomass and plant necrosis (Navari-Izzo et al, 2006; Lequeux et al, 2010; Huang et al, 2016)

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