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Purification method for rabies virus inactivated vaccine

A technology of rabies virus and rabies vaccine, which is applied in the field of purification of rabies virus inactivated vaccine, can solve the problems of virus yield and expanded production that have not been further explored, and achieve the effects of improving consistency, avoiding dilution, and sufficient operating space

Pending Publication Date: 2020-06-09
WUHAN INST OF BIOLOGICAL PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Zeng Rongfang et al [13] As early as 1997, Sepharose CL-6B and DEAE exchange resin were used as media to try a small-scale trial production process of vaccines, but the virus yield and expanded production of this process have not been further explored

Method used

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  • Purification method for rabies virus inactivated vaccine
  • Purification method for rabies virus inactivated vaccine
  • Purification method for rabies virus inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1, production and chromatographic purification of rabies vaccine

[0063] 1. Cells and viruses

[0064] Vero cells: from the American Type Culture Collection (ATCC CCL-81), with a passage of 134 generations. The rabies vaccine laboratory of our company will pass the passage, establish the main cell bank and the working cell bank, and the cell generation for production shall not exceed 150 generations.

[0065] Rabies virus strain CTN-1V strain: purchased from China Institute for the Control of Pharmaceutical and Biological Products, the 23rd generation. The rabies vaccine laboratory of the company has established a main seed bank and a working seed bank. The batches of working seeds used for production do not exceed 35 generations, and the virus titer is 7.5-8.0LgLD50 / ml.

[0066] 2. Vero cell culture

[0067] (1) Take one or several cell tubes from the same batch in the working cell bank, resuscitate and amplify the cells until they are inoculated with th...

Embodiment 2

[0091] Embodiment 2, the effect evaluation of purified vaccine

[0092] 1. Detection of the nature of the vaccine

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Abstract

The invention relates to a purification method for a rabies virus inactivated vaccine. The method includes the following steps: (1) inoculating and cultivating rabies vaccine strains by using Vero cells as virus hosts, and harvesting a virus concentrate; (2) performing virus inactivation; and (3) preparing the purified inactivated rabies vaccine by using molecular sieve chromatography and anion exchange chromatography. The method provided by the invention does not add any chemical purification agent to the vaccine, the operation is simple, the process consistency is good, and the virus antigencan be prevented from being diluted in the purification process.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a purification method of an inactivated rabies virus vaccine. Background technique [0002] Rabies (Rabies) is an acute infectious disease caused by rabies virus, which is a natural foci or animal origin zoonotic. At present, vaccination is still the most effective way to prevent and control rabies. Since Louis Pasteur invented the first human rabies vaccine in 1885, the development of rabies vaccines has gone through early animal nerve tissue vaccines, poultry embryo vaccines, and crude cell cultured vaccines to the current primary hamster kidney vaccine. Cells, primary chicken embryo cells, primary duck embryo cells, African green monkey kidney cells, rhesus monkey fetal diploid cells, and purified vaccines cultured from human diploid cells. [0003] Before 1980, the adult sheep brain tissue vaccine was produced and used in my country, and the adverse reactions after vaccination ...

Claims

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Application Information

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IPC IPC(8): A61K39/205A61P31/14C12N7/00
CPCA61K39/12A61P31/14C12N7/00A61K2039/5252C12N2760/20134C12N2760/20151C12N2760/20163
Inventor 张智刘佐兵梁婧徐晓王玥王玲林弦彭小欢袁莎苏永福姜海天李榆杨刘翠丽金文芳
Owner WUHAN INST OF BIOLOGICAL PROD CO LTD
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