Process for extracting dendrobium polysaccharide and dendrobine from dendrobium officinale

Abstract

The invention provides a process for extracting dendrobium polysaccharide and dendrobine from dendrobium officinale. The process comprises the following steps: (1) cleaning, drying and crushing dendrobium officinale; (2) carrying out supercritical CO2 extraction on the dendrobium nobile powder to obtain an extracting solution A; (3) extracting the extracting solution A, concentrating under reducedpressure, and drying to obtain dendrobine; (4) carrying out centrifugal separation after dendrobium residue water impregnation, and evaporating and concentrating the supernatant to obtain an extracting solution B; (5) carrying out suction filtration on the alcohol precipitation solution of the extracting solution B and then keeping precipitates; (6) dissolving the precipitates in distilled water,adding protease for enzymolysis, adding a trichloroacetic acid solution after enzymolysis, standing for precipitation, filtering and collecting filtrate; and (7) adding activated carbon into the filtrate, standing, filtering, collecting the filtrate, and carrying out freeze drying on the filtrate to obtain the dendrobium polysaccharide. According to the method, step-by-step extraction of the dendrobium polysaccharide and the dendrobine in the dendrobium officinale is achieved, the extraction rate of the dendrobium polysaccharide and the dendrobine is high, and the purity of the obtained dendrobium polysaccharide and the dendrobine is high.

Description

technical field [0001] The invention relates to the technical field of dendrobium processing, in particular to an extraction process of dendrobium polysaccharides and dendrobine in dendrobium candidum. Background technique [0002] Dendrobium candidum is a herbaceous plant of Orchidaceae, which grows on semi-shady rocks in mountains at an altitude of 1,600 meters. Mainly distributed in Anhui, Zhejiang, Fujian and other places in China. Dendrobium officinale has the effects of nourishing yin and nourishing the stomach, moistening the lungs, relieving cough and clearing heat and improving eyesight. It has the functions of dilating blood vessels and enhancing human immunity, so it is called life-saving celestial grass. [0003] Modern pharmacological studies have proved that the main active ingredients contained in Dendrobium officinale are Dendrobium polysaccharides, dendrobine, amino acids, stilbenes and their derivatives, trace elements and various volatile components. De...

AI Technical Summary

Problems solved by technology

The existing extraction methods of Dendrobium polysaccharides mainly include heating extraction method, enzymatic hydrolysis method, ultrasonic-assisted extraction method, microwave-assisted extraction method, etc. There are also many extraction methods for Dendrobium, and the conventional extraction methods mainly include maceration method, percolation method, and reflux method. , microwave-assisted extraction, ultrasonic extraction and other methods, but at the same time, there are fewer methods for extracting polysaccharides and alkaloids of dendrobium, and polysaccharides and alkaloids of dendrobium interfere with each other when they are extracted, and proteins and pigments in dendrobium candidum are also mixed in the extracted dendrobium Polysaccharides and dendrobine affect the quality of the product