Method for producing adenosine by fermentation
A technology of adenosine and fermentation strains, applied in the field of microbial fermentation
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Embodiment 1
[0029] A method for producing adenosine by fermentation, the specific operation steps are as follows:
[0030] The components of the fermentation medium are: sucrose 80g / L, ammonium sulfate 12g / L, yeast extract 8g / L, sodium glutamate 5g / L, magnesium sulfate heptahydrate 3g / L, potassium dihydrogen phosphate 1g / L, a Manganese sulfate water 100mg / L, ferrous sulfate heptahydrate 50mg / L, soybean oil 10ml / L, pH 6.8;
[0031] Taking Bacillus subtilis ATCC21616 as an example, the seed solution (OD 600 The value is 8) connected to a 500L fermenter with 300L fermentation medium, the culture temperature is 33°C, the dissolved oxygen is 20%, the pH is controlled by automatic flow of ammonia water, 0-24h, the pH is controlled at 6.8, 24h to At the end, the pH is controlled to be 6.5; a sucrose solution with a mass fraction of 50% is added to control the residual sugar content in the fermentation broth to be not less than 1g / L; the total fermentation time is 48h to obtain an adenosine ferm...
Embodiment 2
[0034] A method for producing adenosine by fermentation, the specific operation steps are as follows:
[0035] The components of the fermentation medium are: sucrose 80g / L, ammonium sulfate 10g / L, yeast extract 7g / L, sodium glutamate 5g / L, magnesium sulfate heptahydrate 2g / L, potassium dihydrogen phosphate 1g / L, a Manganese sulfate water 120mg / L, ferrous sulfate heptahydrate 40mg / L, soybean oil 12ml / L, pH 6.8;
[0036] Taking Bacillus subtilis ATCC21616 as an example, the seed solution (OD 600 The value is 8) connected to a 500L fermenter with 300L fermentation medium, the culture temperature is 32.5°C, the dissolved oxygen is 20%, the pH is controlled by automatic flow of ammonia water, 0-24h, the pH is controlled at 6.8, 24h to At the end, the pH is controlled to be 6.5; a sucrose solution with a mass fraction of 50% is added to control the residual sugar content in the fermentation broth to be not less than 1g / L; the total fermentation time is 50h to obtain an adenosine fe...
Embodiment 3
[0039] A method for producing adenosine by fermentation, the specific operation steps are as follows:
[0040] The components of the fermentation medium are: sucrose 70g / L, ammonium sulfate 15g / L, yeast extract 6g / L, sodium glutamate 4g / L, magnesium sulfate heptahydrate 4g / L, potassium dihydrogen phosphate 1.5g / L, Manganese sulfate monohydrate 150mg / L, ferrous sulfate heptahydrate 60mg / L, soybean oil 11ml / L, pH 6.8;
[0041] Taking Bacillus subtilis ATCC21616 as an example, the seed solution (OD 600 The value is 8) connected to a 500L fermenter with 300L fermentation medium, the culture temperature is 32°C, the dissolved oxygen is 20%, the pH is controlled by automatic flow of ammonia water, 0-24h, the pH is controlled at 6.8, 24h to At the end, the pH is controlled to be 6.5; sucrose solution with a mass fraction of 50% is added to control the residual sugar content in the fermentation broth to be not less than 1g / L; the total fermentation time is 52h to obtain an adenosine ...
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