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3D printed skin and preparation method thereof

A 3D printing and skin technology, which is applied in the field of 3D bioprinting, can solve the problems affecting the shape of the scaffold, cell damage, tilting and collapse of the scaffold, etc., and achieve the effect of reducing the molding time

Active Publication Date: 2020-06-12
NANJING NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many scientific researchers use 3D bioprinting technology to manufacture tissue-engineered skin, but the following problems generally exist: (1) The curing molding method or using the temperature-sensitive properties of the material to cure it layer by layer, the defect is that the molding time is too long, which may It will cause the collapse of the stent structure and affect the performance of the formed stent; or compound the photosensitizer into the material, and use ultraviolet light to irradiate the extruded material while printing. Although the molding speed is improved, it may contain toxic components. Photosensitizers and long-term ultraviolet light exposure will cause damage to the cells in the material; (2) Most tissue-engineered skins are produced according to specific parameters, which may cause deviations in characteristics such as shape, size, and pore structure; (3) The mixing of the two-component materials may not be sufficient, resulting in the lack of effective contact in some areas of the mixed material, which cannot quickly cross-link and solidify, resulting in tilted collapse of the stent and affecting the shape of the stent

Method used

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  • 3D printed skin and preparation method thereof
  • 3D printed skin and preparation method thereof
  • 3D printed skin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1: Using an external nozzle with an aperture of 0.46mm to print skin

[0060] (1) 3D scanning and skin modeling

[0061] Use a 3D scanner to scan the damaged wound of human skin (2cm*2cm*6mm) to obtain the three-dimensional structure of the damaged skin, and then use the computer-aided design software solidworks to complete the three-dimensional layered modeling of the damaged part to obtain the skin 3D skin model suitable for damaged parts.

[0062] (2) Equipment of materials

[0063] 1) Preparation of polyamino acid hydrogel

[0064] i. To modify the polymer side chain of polyglutamic acid, dissolve 4g of γ-polyglutamic acid (0.031mol) in 50ml of 0.1mol / L MES buffer (PH=5), and stir until a clear solution is formed Finally, add 0.96g EDC (0.005mol) and 0.58g NHS (0.005mol) to activate polyglutamic acid at 37°C for 80 minutes, add 1g adipic acid dihydrazide and 0.5g cysteine ​​hydrochloride (the molar ratio of the two is 1:0.77), react at 37°C for 24 hours,...

Embodiment 2

[0089] Embodiment 2: In this embodiment, the skin is printed by means of screw pushing and coaxial extrusion, such as Figure 5 As shown, 51 is a coaxial rubber cartridge, and 52 is a coaxial rubber cartridge nozzle; 51 is selected as the extrusion rubber cartridge, and the rest are the same as in Example 1, and the final molding time is 18 minutes. Among them, because the coaxial rubber cylinder is not designed with a corresponding external spiral nozzle, and the contact of the two materials in the coaxial extrusion method is not as sufficient as that of the two-component rubber cylinder. Therefore, the time of Example 2 is slightly longer than that of Comparative Example 1.

Embodiment 3

[0090] Embodiment 3: In this embodiment, the skin is printed by means of pneumatic push and 1:1 Y-type mixed extrusion, such as image 3 As shown, 31 is the push rod of the pneumatic glue gun, and 32 is the fixed position of the rubber cartridge; the pneumatic glue gun is fixed on 13 of the 3D bioprinter, and 41 is selected as the extrusion glue cartridge. The XYZ-axis motion module 11 of the 3D bioprinter controls the pneumatic glue gun to run on a specific trajectory. The bottom of the pneumatic glue gun is connected to the air pipe, and the air pressure pushes the push rod 31 of the pneumatic glue gun, which pushes the piston in the rubber cartridge for stable and uniform extrusion. The material is sprayed in a spiral shape and the final molding time is 12 minutes.

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Abstract

The invention discloses 3D printed skin and a preparation method thereof. The skin is composed of an artificial epidermal layer, an acellular dermal scaffold and an artificial dermal layer from top tobottom. The 3D printed skin is characterized in that the skin is prepared from polyamino acid hydrogel and hyaluronic acid hydrogel through two-component 3D printing. Compared with the prior art, theinvention has the following advantages that: (1) the skin is formed by composite printing of two-component materials, the forming speed is high, the spiral external spray head is adopted to further shorten the printing forming time, and collapse is not easily caused in the printing process, and the shape retentivity is good; and (2) the proper shape, size and pore structure determined by scanningare adopted, and the acellular dermal scaffold is added, so that cell ingrowth and healing of wounds are facilitated, and a certain degree of mechanical support is provided.

Description

technical field [0001] The invention relates to the field of 3D bioprinting in tissue engineering, in particular to a 3D printed skin and a preparation method thereof. Background technique [0002] The skin is the largest organ of the human body, an important tissue covering the body surface, and a barrier between the human body and the external environment. It not only undertakes functions such as protecting the body, sweating, and sensing, but also maintains the stability of the internal environment of the body. For some minor skin injuries, the skin can heal and recover by itself; but when the skin suffers a large area of ​​serious injury (such as burns), if only the superficial layer of the skin is damaged, after the wound is treated in normal healthy people, new skin will regenerate ; but for some patients with diseases, such as diabetes, it is obviously not realistic to recover by the human body. At this time, the damaged skin of the patient needs to be transplanted. I...

Claims

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Application Information

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IPC IPC(8): A61L27/60A61L27/36A61L27/20A61L27/18A61L27/52A61L27/50A61L27/54A61L27/56B33Y10/00B33Y70/00B33Y80/00
CPCA61L27/60A61L27/362A61L27/20A61L27/18A61L27/52A61L27/50A61L27/54A61L27/56B33Y10/00B33Y70/00B33Y80/00A61L2430/40A61L2300/404A61L2300/216C08L5/08C08L77/04
Inventor 朱莉娅朱国涛程明唐文来杨继全
Owner NANJING NORMAL UNIVERSITY