Method for preparing ganoderma lucidum spore powder wall shell crude polysaccharide by using enzymic-method assisted ultrasound

A technology of Ganoderma lucidum spore powder and Ganoderma lucidum spores, which can be used in sustainable manufacturing/processing, chemical industry, climate sustainability, etc., and can solve problems such as waste of resources

Active Publication Date: 2020-06-12
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing studies have shown that the effective substances in Ganoderma lucidum spore powder are difficult to be digested and absorbed by the human body without breaking the wall, so the current extraction process of Ganoderma lucidum spore powder is roughly as follows: Ganoderma lucidum spore powder wall breaking-water heating extraction-degreasing treatment, the remaining cell wall Ganoderma lucidum spore shell, currently the shell of Ganoderma spore powder is treated as waste, resulting in a great waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0035] Sample No. 1 was crushed through a 100-mesh sieving equipment to obtain ganoderma spore shell powder with a particle size greater than 100 mesh; accurately weighed 0.5 g of ganoderma spore shell powder, and mixed 0.02 g of cellulase and 0.02 g of hemicellulose Enzyme and 0.08g of chitinase were mixed to obtain a compound enzyme, and the compound enzyme was added to Ganoderma lucidum spore shell powder for mixing to obtain a mixture; 20mL of pH5 buffer solution was added to the mixture and placed in a constant temperature incubator at 50°C Carry out the reaction, after 4 hours of enzymolysis, carry out ultrasonic treatment, ultrasonic power 250W, ultrasonic treatment 60min under the condition of ultrasonic temperature 50 ℃, take it out, obtain enzymatic hydrolysis solution; After enzymolysis solution is inactivated by boiling water for 10min, carry out centrifugation (3500r / min, 5min) to filter to obtain the filtered separation liquid, take the supernatant of the filtere...

Embodiment example 2

[0037] After crushing sample No. 2, pass through a 100-mesh sieving equipment to obtain Ganoderma lucidum spore shell powder with a particle size greater than 100 mesh; accurately weigh 0.5g of Ganoderma lucidum spore shell powder, mix 0.03g of cellulase, 0.03g of hemifiber Sulfase and 0.04g of chitinase are mixed to obtain a compound enzyme, and the compound enzyme is mixed with Ganoderma lucidum spore shell powder evenly to obtain a mixture; after adding 15 mL of pH5 buffer solution to the mixture, place it in a constant temperature incubator at 45°C Carry out the reaction, take it out after enzymolysis for 2 hours, and obtain the enzymolysis solution; add 2 times the volume of absolute ethanol to carry out alcohol precipitation for 48 hours after the enzymolysis solution is inactivated, and obtain the precipitation separation solution, and collect the bottom layer of the precipitation separation solution Concentrate and dry the product, and finally weigh the ganoderma crude ...

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PUM

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Abstract

The invention discloses a method for preparing ganoderma lucidum spore powder wall shell crude polysaccharide by using an enzymic-method assisted ultrasound. According to the technical scheme, the method comprises the following steps of screening crushed ganoderma lucidum spore powder wall shells through screening equipment, and acquiring a ganoderma lucidum spore wall shell powder with a powder diameter larger than 100 meshes; treating the ganoderma lucidum spore wall shell powder with a compound enzyme, then carrying out ultrasonic treatment, then inactivating and filtering, and taking a supernatant to obtain an extracting solution of ganoderma lucidum spore powder wall shell crude polysaccharide; adding absolute ethyl alcohol into the extracting solution for alcohol precipitation, and obtaining a precipitation separation solution; and taking a bottom-layer precipitate of the precipitation separation solution to be concentrated and dried, and finally obtaining the ganoderma lucidum spore powder wall shell crude polysaccharide. A yield of the extracted ganoderma lucidum crude polysaccharide is as high as 11.25%, efficiency is improved by about 100 times compared with that of direct extraction of the crude polysaccharide, and meanwhile, the crude polysaccharide has a moisturizing effect and multiple immune functions and can be widely applied to health care products, beauty markets and the like.

Description

technical field [0001] The invention relates to the technical field of extraction of fungal effective substances, in particular to a method for preparing ganoderma lucidum spore powder wall shell crude polysaccharide by using an enzyme method to assist ultrasound. [0002] technical background [0003] Ganoderma lucidum spores are extremely tiny ovoid reproductive cells ejected from the gills of Ganoderma lucidum during the growth and maturity stage of Ganoderma lucidum, that is, the seeds of Ganoderma lucidum. Each spore of Ganoderma lucidum is only 4-6 microns, and it is a living organism with a double-walled structure, surrounded by hard chitin cellulose, and has all the genetically active substances of Ganoderma lucidum. Its spore powder and fruiting body can be used as medicine. The chemical composition of Ganoderma lucidum is very complex, containing active ingredients such as Ganoderma lucidum polysaccharides, triterpenoids, proteins, amino acids, sterols, and alkaloi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04C08B37/00
CPCC12P19/14C12P19/04C08B37/0003Y02P20/10
Inventor 帅玉英俞汾王帅帅
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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