Preparation method of freeze-dried lactobacillus paracasei powder

Abstract

The invention discloses a preparation method of freeze-dried lactobacillus paracasei powder, and belongs to the technical field of probiotic starter culture. The method comprises the following steps:collecting thalli of lactobacillus paracasei, and then mixing the thalli with a freeze-drying protective additive, wherein the freeze-drying protective additive is prepared from skim milk powder, canesugar, polyvinylpyrrolidone, glutathione and water; then paving a mixed solution of thalli and the freeze-drying protective additive in a freeze-drying vessel for pre-freezing treatment, and after the mixture of the thalli and the freeze-drying protective additive are completely frozen, carrying out vacuum freeze-drying treatment on a pre-frozen sample. The survival factor of thalli in the freeze-dried lactobacillus paracasei powder prepared by the preparation method disclosed by the invention reaches 0.998 + / -0.001, the viable count is 2.35 * 10 < 11 > cfu / g, and the viable count content ishigh. And necessary technical support is provided for development of lactobacillus paracasei products and the aspects of dairy products, foods, medicines and the like.

Description

technical field [0001] The invention relates to a preparation method of freeze-dried bacteria powder of Lactobacillus paracasei, belonging to the technical field of probiotic starters. Background technique [0002] Lactobacillus paracasei belongs to the Lactobacillus casei group in the genus Lactobacillus, widely exists in fermented foods such as cheese, pickles and human gastrointestinal tract, has good acid resistance and bile resistance, and can reduce plasma Cholesterol has physiological functions such as promoting the elimination of pathogens in the human intestinal tract, treating intestinal flora disorders, preventing food allergies and acute diarrhea, and preventing tumors. [0003] In recent years, with the continuous research on the mechanism of action of lactic acid bacteria, lactic acid bacteria have been widely used in many fields such as food industry, pharmaceutical industry, light industry and livestock and poultry production industry, which also promoted the...

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that the existing freeze-drying process has caused great damage to Lactobacillus paracasei, resulting in low bacterial activity and low number of viable bacteria in the freeze-dried bacterial powder, the invention provides a preparation method for the freeze-dried bacterial powder of Lactobacillus paracasei , the method can improve the freeze-dried survival factor of Lactobacillus paracasei and the viable count of bacteria powder, and the technical scheme adopted is as follows:

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