Sustained-release composition preparation of polypeptide protein drugs and preparation method thereof
A slow-release composition and peptide protein technology, which is applied in the fields of peptide/protein components, drug delivery, pharmaceutical formulations, etc., can solve the problems of complex formulation design, achieve structure and biological activity, high drug content, and stability Effect
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Embodiment 1
[0041] Accurately weigh 200mg PLGA1500-PEG1450-PLGA1500 (both are number average molecular weight, the weight percentage of PEG block is 32.6%, the weight percentage of PLGA block is 67.4%, the molar ratio of LA / GA is 3:1), add 0.80ml to remove Ionized water was dissolved under magnetic stirring at room temperature to obtain a temperature-sensitive polymer aqueous solution with a concentration of 20% (w / w), and an LCST of 32°C. Accurately weigh 1mg of recombinant human growth hormone (rhGH), add 0.3ml of water to dissolve to obtain rhGH aqueous solution; accurately weigh 1mg of protamine (Tokyo Chemical Industry Co., Ltd. (TCI)), add 0.3ml of water to dissolve to obtain protamine aqueous solution; under magnetic stirring conditions, the protamine aqueous solution was added dropwise to the rhGH aqueous solution, and mixed uniformly to obtain a suspension of the rhGH complex; the suspension of the rhGH complex was centrifuged, and the supernatant was removed, that is The rhGH co...
Embodiment 2
[0043] Accurately weigh 200mg of PLGA1200-PEG1000-PLGA1200 (both are number-average molecular weight, the weight percent of PEG block is 29.4%, the weight percent of PLGA block is 70.6%, and the molar ratio of LA / GA is 3:1), add 0.80ml of Dissolve in deionized water under magnetic stirring at 15°C to obtain a temperature-sensitive polymer aqueous solution with a concentration of 20% (w / w), and an LCST of 20°C. Accurately weigh 1 mg of recombinant human growth hormone, add 0.3 ml of water to dissolve it, and obtain rhGH aqueous solution; accurately weigh 1 mg of protamine (Tokyo Chemical Industry Co., Ltd. (TCI)), add 0.3 ml of water to dissolve it, and obtain protamine Aqueous solution; add the protamine aqueous solution dropwise to the rhGH aqueous solution under stirring, mix evenly to obtain a suspension of the rhGH complex; centrifuge the suspension of the rhGH complex, remove the supernatant, and obtain the rhGH complex things. The rhGH complex and the temperature-sensit...
Embodiment 3
[0045] Accurately weigh 200 mg of PLGA1500-PEG1450-PLGA1500 (both are number average molecular weight, the weight percentage of PEG block is 32.6%, the weight percentage of PLGA block is 67.4%, the molar ratio of LA / GA is 3:1), add 0.80 ml of deionized water was dissolved under magnetic stirring at 15°C to obtain a temperature-sensitive polymer aqueous solution with a concentration of 20% (w / w), and an LCST of 32°C. Accurately weigh 1mg of recombinant human growth hormone, add 0.3ml of water to dissolve, obtain rhGH aqueous solution; accurately weigh 1mg of zinc acetate, add 0.3ml of water to dissolve, obtain zinc acetate aqueous solution; accurately weigh 1mg of protamine (Tokyo Chemical Industry Co., Ltd. (TCI)), add 0.3ml of water to dissolve to obtain an aqueous solution of protamine; add the aqueous solution of zinc acetate and aqueous solution of protamine dropwise to the aqueous solution of rhGH under stirring, and mix evenly to obtain a rhGH complex The suspension of t...
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