An electrode system for electrochemical detection and its application

An electrode system and electrode technology, applied in the direction of electrochemical variables of materials, measuring devices, scientific instruments, etc., can solve the problems of high manufacturing cost of non-enzymatic electrodes, failure to meet actual detection requirements, and narrow detection range of sensors, and achieve good resistance. Interference ability and selectivity, wide detection concentration range, effect of wide detection range

Active Publication Date: 2022-03-29
SHANXI UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0003] Among many glutamic acid detection methods, electrochemical detection has higher accuracy and sensitivity, but the current electrochemical glutamic acid detection methods have the following problems: 1) The preparation process of enzyme-based electrochemical sensors is complicated, the cost is high, Problems such as low sensitivity and poor enzyme stability lead to a narrow detection range of this type of sensor and more restrictions on use conditions; 2) non-enzyme electrodes such as Pt / Ni nanowire arrays can only achieve 10 2 μA / mM·cm 2 sensitivity and 10 -6 The linear detection range in the mol / L range is far from the actual detection requirements, and the manufacturing cost of the non-enzyme electrodes in the prior art is high

Method used

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  • An electrode system for electrochemical detection and its application
  • An electrode system for electrochemical detection and its application
  • An electrode system for electrochemical detection and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0045] 1) Add chitosan powder in the acetic acid aqueous solution whose concentration is 0.3mol / L, prepare the chitosan-acetic acid solution that concentration is 10mg / mL. Pour this solution into a cylindrical glass beaker, freeze at -20°C for 24 hours, and dry it in a freeze dryer at -80°C to obtain a cylindrical chitosan foam and a shell prepared from chitosan-acetic acid solution. SEM images of glycan foam as figure 1 shown. The chitosan foam was placed in a tube furnace and calcined to 900°C at a heating rate of 5°C / min under an argon atmosphere and maintained for 2h. SEM images of chitosan-derived carbon foams prepared from chitosan-acetic acid solution as figure 2 shown.

[0046] 2) The chitosan-derived porous carbon foam was cut into 1 mm circular slices, fixed on the gold sheet current collector with conductive carbon tape as the working electrode, the silver / silver chloride electrode was used as the reference electrode, and the platinum sheet electrode was used as...

Embodiment 2

[0057] 1) Add chitosan powder in the acetic acid aqueous solution whose concentration is 0.3mol / L, prepare the chitosan-acetic acid solution that concentration is 10mg / mL. Pour the solution into a cylindrical glass beaker, freeze at -20°C for 24 hours at a constant temperature, and dry in a freeze dryer at -80°C to obtain a cylindrical chitosan foam. The chitosan foam was placed in a tube furnace and calcined to 900°C at a heating rate of 5°C / min under an argon atmosphere and maintained for 2h.

[0058] 2) The chitosan-derived porous carbon foam was cut into 1 mm circular slices, fixed on the gold sheet current collector with conductive carbon tape as the working electrode, the silver / silver chloride electrode was used as the reference electrode, and the platinum sheet electrode was used as the counter electrode. electrodes to form a three-electrode system.

[0059] 3) Prepare 1.4mmol / L KH 2 PO 4 , 4.3mmol / L Na 2 HPO 4 , a mixed aqueous solution of 137mmol / L NaCl and 2.7m...

Embodiment 3

[0064] 1) Add chitosan powder in the acetic acid aqueous solution that concentration is 0.5mol / L, prepare the chitosan-acetic acid solution that concentration is 10mg / mL. Pour the solution into a cylindrical glass beaker, freeze at -20°C for 12 hours at a constant temperature, and dry in a freeze dryer at -80°C to obtain a cylindrical chitosan foam. The chitosan foam was placed in a tube furnace and calcined to 900°C at a heating rate of 10°C / min under an argon atmosphere and maintained for 3h.

[0065] 2) The chitosan-derived porous carbon foam was cut into 1 mm circular slices, fixed on the gold sheet current collector with conductive carbon tape as the working electrode, the silver / silver chloride electrode was used as the reference electrode, and the platinum sheet electrode was used as the counter electrode. electrodes to form a three-electrode system.

[0066] 3) Prepare 1.4mmol / L KH 2 PO 4 , 4.3mmol / L Na 2 HPO 4 , a mixed aqueous solution of 137mmol / L NaCl and 2.7m...

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Abstract

The invention provides a preparation method of a chitosan-derived porous carbon foam electrode, comprising: 1) mixing chitosan powder and an aqueous acetic acid solution uniformly to obtain a chitosan-acetic acid solution, and freezing and drying the chitosan-acetic acid solution at a low temperature Finally, chitosan foam is obtained; 2) calcining the chitosan foam in an inert atmosphere to obtain chitosan derived porous carbon foam; 3) slicing the chitosan derived porous carbon foam to obtain chitosan derived porous carbon foam A carbon foam sheet, the chitosan-derived porous carbon foam sheet is fixed on a metal current collector through a conductive medium to obtain a chitosan-derived porous carbon foam electrode. The invention also provides its application in detecting glutamic acid. The invention has the advantages of low cost, high sensitivity, wide detection range, low detection limit, good anti-interference performance, good stability and the like.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and specifically relates to a chitosan-derived porous carbon foam electrode system, in particular to a glutamic acid with simple production, non-enzyme, wide detection concentration range, high sensitivity and strong anti-interference ability Detection method. Background technique [0002] Glutamic acid is a very important substance in the human body. Plays an important role in neural transmission in vertebrates. Numerous studies have shown that changes in the concentration of glutamate in the human body may be related to many neurological diseases, such as stroke, autism, some forms of intellectual disability, Huntington's disease, etc. In addition, glutamic acid is also widely used as a food additive, but if taken in excess, it will cause head and gastrointestinal adverse reactions. Therefore, the detection of glutamic acid plays an important role in the fields of biomedicine an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30
CPCG01N27/308
Inventor 王梅马一飞秦洁玲黎子健陈旭远肖连团贾锁堂
Owner SHANXI UNIV
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