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High-throughput detection method for carotenoid in aquatic product

A technology for carotenoids and detection methods, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as inability to identify carotenoids, and achieve the effect of reducing time and improving detection efficiency

Active Publication Date: 2020-06-19
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most carotenoids have three maximum absorption peaks in ultraviolet light, commonly known as "three-finger peaks", which can analyze the total amount of carotenoids, but cannot analyze the existing forms of carotenoids in the matrix (free, esterified, oxidized). ) progressive identification

Method used

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  • High-throughput detection method for carotenoid in aquatic product
  • High-throughput detection method for carotenoid in aquatic product
  • High-throughput detection method for carotenoid in aquatic product

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Experimental program
Comparison scheme
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specific Embodiment 1

[0052] A high-throughput detection method for carotenoids in aquatic products, comprising the following steps:

[0053] A. Pre-processing:

[0054] (A1) Weigh 1 g of the aquatic product to be tested, add it to 10 mL of methanol solution containing 0.1 wt % 2,6-di-tert-butyl-p-cresol, shake for 5 min, ultrasonically extract for 20 min, and then at 4 °C at a speed of 12000 r / min After centrifugation for 10 min, the organic phase and residue were obtained after completion;

[0055] (A2) Take the organic phase obtained in step (A1), and add at least 4 times the volume of water to it to obtain the sample solution, then purify the sample solution through the HLB column to obtain the purified sample solution, and then blow it with nitrogen Concentrate the purified sample solution to <2.0ml, then dilute to 2.0ml with methanol solution containing 0.1wt% HF to obtain the purified sample solution after constant volume, wherein:

[0056] HLB column purification adopts methanol solution ...

specific Embodiment 2

[0086] A high-throughput detection method for carotenoids in aquatic products, comprising the following steps:

[0087] A. Pre-processing:

[0088] (A1) Weigh 1 g of the aquatic product to be tested, add it to 10 mL of methanol solution containing 0.1 wt % 2,6-di-tert-butyl-p-cresol, shake for 5 min, ultrasonically extract for 20 min, and then at 10 ° C at a speed of 12000 r / min After centrifugation for 5 minutes, the organic phase and residue were obtained after completion;

[0089] (A2) Take the organic phase obtained in step (A1), and add at least 4 times the volume of water to it to obtain the sample solution, then purify the sample solution through the HLB column to obtain the purified sample solution, and then blow it with nitrogen Concentrate the purified sample solution to <2.0ml, then dilute to 2.0ml with methanol solution containing 0.1wt% HF to obtain the purified sample solution after constant volume, wherein:

[0090] HLB column purification adopts methanol solu...

specific Embodiment 3

[0118] Specific embodiment 3 (method one)

[0119] A high-throughput detection method for carotenoids in aquatic products, comprising the following steps:

[0120] A. Pre-processing:

[0121] (A1) Weigh 1 g of the aquatic product to be tested, add it to 10 mL of methanol solution containing 0.1 wt % 2,6-di-tert-butyl-p-cresol, shake for 5 min, ultrasonically extract for 20 min, and then at 5 °C at a speed of 12000 r / min After centrifugation for 8 minutes, the organic phase and residue were obtained after completion;

[0122] (A2) Take the organic phase obtained in step (A1), and add at least 4 times the volume of water to it to obtain the sample solution, then purify the sample solution through the HLB column to obtain the purified sample solution, and then blow it with nitrogen Concentrate the purified sample solution to <2.0ml, then dilute to 2.0ml with methanol solution containing 0.1wt% HF to obtain the purified sample solution after constant volume, wherein:

[0123] H...

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Abstract

The invention discloses a high-throughput detection method for carotenoid in an aquatic product. The method comprises the following steps: A, performing pretreatment; B, detecting a to-be-detected sample solution through a high performance liquid chromatography-quadrupole electrostatic field orbitrap high-resolution mass spectrometer to obtain a high performance liquid chromatogram of the to-be-detected sample solution; C, making a data analysis. The high-throughput detection method for carotenoid in aquatic products disclosed by the invention has the following beneficial effects: 1, the carotenoid determination lower limit is 0.05 mg / kg, and a standard curve is good in a linear range of 10ng / mL-200ng / mL; 2, during the pretreatment, solid-phase extraction and matrix dispersion purification technologies are used; the extraction and purification work of three carotenoids with great physical and chemical property differences is realized through one-time pretreatment, and the expensive time and reagent cost consumption are reduced while the detection efficiency is improved; 3, the method is applied to aquatic products, and 50 carotenoid types are detected in total.

Description

technical field [0001] The invention relates to the field of detection of nutritional components of aquatic products, in particular to a high-throughput detection method for carotenoids in aquatic products. Background technique [0002] Carotenoids are a kind of natural pigment molecules, which mainly exist in fish, shrimp, crab, shells and other aquatic objects. Their structural composition and distribution are important indicators for evaluating the quality of aquatic products. These photosensitive carotenoids not only serve as coloring agents for the body and tissues, but also improve the sensory quality, and can also enhance the adaptability of aquatic organisms to the environment and some important physiological functions, including improving seedling efficiency and disease resistance. Studies have shown that aquatic organisms cannot synthesize carotenoids from scratch, but they can transform or directly absorb dietary carotenoids. Each organism has its own unique carot...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/7233G01N30/8637G01N30/8679G01N2030/062
Inventor 李双陈娟娟徐继林陈海敏杨锐骆其君吴玮
Owner NINGBO UNIV