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Embedded immobilized microorganism particles, preparation method and sewage treatment method

A technology for immobilizing microorganisms and microorganisms, applied in biological water/sewage treatment, sustainable biological treatment, water/sludge/sewage treatment, etc. Convenient and simple, improve purification effect, reduce density effect

Active Publication Date: 2020-06-26
YUNCHENG UNIVERISTY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When traditional microbial technology treats sewage, it usually directly inoculates microorganisms in the sewage to be treated, and reduces the concentration of pollutants through the growth, absorption, and metabolism of microorganisms. However, due to environmental factors, competition, and easy loss, traditional methods The processing effect is not very ideal
[0003] Immobilized microorganism technology locates free organisms in a limited area through physical and chemical actions. Compared with traditional suspension biological treatment, it can purify and maintain high-efficiency strains. However, the current production process of immobilized microorganisms usually still uses microorganisms The species is directly embedded with polyvinyl alcohol or sodium alginate. Due to its unsatisfactory mechanical strength, it often needs to be placed in a mesh metal shell for use. When it is applied to natural water bodies, the metal shell is difficult to recycle. Therefore, its use in black and odorous water bodies in nature is limited. In addition, when polymers are used to immobilize microorganisms, microorganisms will continue to grow in embeddings, resulting in a high damage rate of embeddings, which also limits its use to a certain extent. Large-scale application in urban sewage biological treatment

Method used

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  • Embedded immobilized microorganism particles, preparation method and sewage treatment method
  • Embedded immobilized microorganism particles, preparation method and sewage treatment method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A preparation method for embedding immobilized microbial particles, characterized in that it specifically comprises the following steps:

[0041] Step 1. Adsorption of microorganisms

[0042] Step 1-1, preparation and activation of adsorbent,

[0043] Soak the nano-carbon with 1mol / L hydrochloric acid solution for 48 hours, rinse it with distilled water until it is neutral, and filter it, then put it in an oven and dry it at 105°C until it reaches a constant weight, which is the adsorbent. Reserve in the desiccator;

[0044] Step 1-2, suspension of microbial cells

[0045] Centrifuge the activated and fermented seed liquid of Pseudomonas shiitake to obtain Pseudomonas thallus, and suspend the microorganisms according to the mass ratio of Pseudomonas thallus (in terms of dry weight) to liquid medium at a mass ratio of 1:9 In the liquid culture medium, the microbial suspension solution is obtained;

[0046] Step 1-3, Adsorption

[0047] According to the 1:1 ratio of ...

Embodiment 2

[0053] A preparation method for embedding immobilized microbial particles, characterized in that it specifically comprises the following steps:

[0054] Step 1. Adsorption of microorganisms

[0055] Step 1-1, preparation and activation of adsorbent,

[0056] Soak the nano-carbon with 1mol / L hydrochloric acid solution for 48 hours, rinse it with distilled water until it is neutral, and filter it, then put it in an oven and dry it at 105°C until it reaches a constant weight, which is the adsorbent. Reserve in the desiccator;

[0057] Step 1-2, suspension of microbial cells

[0058] Centrifuge the activated and fermented seed liquid of Pseudomonas shiitake to obtain Pseudomonas thallus, and suspend the microorganisms according to the mass ratio of Pseudomonas thallus (in terms of dry weight) to liquid medium at a mass ratio of 1:9 In the liquid culture medium, the microbial suspension solution is obtained;

[0059] Step 1-3, Adsorption

[0060] According to the 1:1 ratio of mi...

Embodiment 3

[0066] A preparation method for embedding immobilized microbial particles, characterized in that it specifically comprises the following steps:

[0067] Step 1. Adsorption of microorganisms

[0068] Step 1-1, preparation and activation of adsorbent,

[0069] Soak the nano-carbon with 1mol / L hydrochloric acid solution for 48 hours, rinse it with distilled water until it is neutral, and filter it, then put it in an oven and dry it at 105°C until it reaches a constant weight, which is the adsorbent. Reserve in the desiccator;

[0070] Step 1-2, suspension of microbial cells

[0071] Centrifuge the activated and fermented seed liquid of Pseudomonas shiitake to obtain Pseudomonas thallus, and suspend the microorganisms according to the mass ratio of Pseudomonas thallus (in terms of dry weight) to liquid medium at a mass ratio of 1:9 In the liquid culture medium, the microbial suspension solution is obtained;

[0072] Step 1-3, Adsorption

[0073] According to the 1:1 ratio of ...

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Abstract

The invention relates to embedded immobilized microorganism particles, a preparation method and a sewage treatment method. The preparation method comprises the following steps: 1, suspending microorganisms in a liquid culture medium to obtain a microorganism suspension solution; slowly pouring into an adsorbent, stirring, standing for 2 hours, and centrifuging after the adsorbent fully adsorbs themicroorganism suspension solution to obtain an adsorbent adsorbed with microorganisms; 2, dissolving polyvinyl alcohol and agar in water, adding an adsorbent adsorbed with microorganisms, stirring uniformly, cooling, and cutting into particles to obtain immobilized microorganism particles; and step 3, uniformly mixing the cement, the porous ore, the porous carbon fiber and the organic silicon cross-linking agent, adding water into the mixture, continuously stirring until the mixture is uniformly mixed, spraying the mixture on the surfaces of the immobilized microorganism particles, and curing. After the microorganisms are embedded and immobilized by adopting the method, the survival rate of the microorganisms in sewage is high, and the sewage treatment effect is good.

Description

technical field [0001] The invention belongs to the field of sewage treatment, and in particular relates to an embedded and immobilized microbial particle, a preparation method and a sewage treatment method. Background technique [0002] In order to deal with the increasingly serious pollution problem, scientists from all over the world are trying to find water treatment technologies with low cost, low energy consumption and low secondary pollution. Due to the unique role of microorganisms in the removal of pollutants, biodegradation is regarded as a relatively effective, economical and energy-saving water treatment technology. When traditional microbial technology treats sewage, it usually directly inoculates microorganisms in the sewage to be treated, and reduces the concentration of pollutants through the growth, absorption, and metabolism of microorganisms. However, due to environmental factors, competition, and easy loss, traditional methods The processing effect is no...

Claims

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Application Information

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IPC IPC(8): C02F3/34
CPCC02F3/34Y02W10/10
Inventor 张晓斌
Owner YUNCHENG UNIVERISTY
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