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Method for evaluating authenticity of lotus bee pollen

A technology for lotus bee pollen and bee pollen, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve problems such as deceiving consumers, and achieve the effect of easy operation and promotion and high content

Active Publication Date: 2020-06-26
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to pursue high profits, some unscrupulous companies often use other varieties of pollen as lotus bee pollen to deceive consumers
At present, the industry has not formulated any standards or industry standards related to lotus bee pollen, and the lack of relevant standards and identification methods provides the possibility of adulteration in the current lotus bee pollen market

Method used

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  • Method for evaluating authenticity of lotus bee pollen
  • Method for evaluating authenticity of lotus bee pollen
  • Method for evaluating authenticity of lotus bee pollen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] 1. Sample source

[0056] A total of 24 common real bee pollen samples were purchased from the market or beekeepers, namely rape bee pollen (3 parts), camellia bee pollen (3 parts), lotus bee pollen (3 parts), corn bee pollen (3 parts) , Rose Bee Pollen (3 parts), Codonopsis Bee Pollen (3 parts), Schisandra Bee Pollen (3 parts), Buckwheat Bee Pollen (3 parts). It is used to detect the content of biflavonoids in various bee pollens.

[0057] 2. sample handling

[0058] Grind bee pollen into powder, weigh 0.8 g of ground bee pollen, add 7.2 mL of extraction solution, vortex at room temperature until completely dissolved, and g , centrifuged at 4°C for 20 min. Collect the supernatant and pass it through a 0.22 µm filter; absorb 10 µL of the extract and dilute once with 990 µL of methanol-water; draw another 100 µL of extract: dilute once with 900 µL of methanol-water. The diluent was transferred to a sample vial for analysis on the machine.

[0059] 3. Q Exactive plu...

Embodiment 2

[0067] 1. Sample source

[0068] A total of 24 common real bee pollen samples were purchased from the market or beekeepers, namely rape bee pollen (3 parts), camellia bee pollen (3 parts), lotus bee pollen (3 parts), corn bee pollen (3 parts) , Rose Bee Pollen (3 parts), Codonopsis Bee Pollen (3 parts), Schisandra Bee Pollen (3 parts), Buckwheat Bee Pollen (3 parts). It is used to detect the content of biflavonoids in various bee pollens.

[0069] 2. Solution preparation

[0070] (1) Solution preparation

[0071] Extraction solution: pipette 20 mL of methanol, and dilute to 100 mL with ultrapure water. Store at 4°C.

[0072] Standard solution of abiflavones from A. spiconiae: Weigh 10 mg of abiflavones from A. spiconiae standard, and dilute to 10 mL with methanol. Store at 4°C, valid for 2 months.

[0073] 3. Q Exactive plus test conditions are as follows:

[0074] (1) Sample processing:

[0075] Grind bee pollen into powder, weigh 0.8 g of ground bee pollen, add 7.2 m...

Embodiment 3

[0097] 1. Sample source

[0098] Buy 20 kinds of commercial lotus bee pollen from the market.

[0099] 2. Experimental procedure

[0100] (1) Solution preparation

[0101] Extraction solution: pipette 20 mL of methanol, and dilute to 100 mL with ultrapure water. Store at 4°C.

[0102] AF standard solution: Weigh 10 mg of AF standard and dilute to 10 mL with methanol. Store at 4 ℃, valid for 2 months.

[0103] (2) Sample processing:

[0104] Grind bee pollen into powder, weigh 0.8 g of ground bee pollen, add 7.2 mL of extraction solution, vortex at room temperature until completely dissolved, and g , centrifuged at 4°C for 20 min. Collect the supernatant and pass it through a 0.22 µm filter; absorb 10 µL of the extract and dilute once with 990 µL of methanol-water; draw another 100 µL of extract: dilute once with 900 µL of methanol-water. The diluent was transferred to a sample vial for analysis on the machine.

[0105] (3) Blank bee pollen spiked:

[0106] Grind blan...

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Abstract

The invention relates to the field of food detection, in particular to a method for evaluating authenticity of lotus bee pollen. According to the invention, firstly, the application of high-content amentoflavone (AF) as a lotus bee pollen characteristic marker is discovered. Accordingly, the invention provides the method for evaluating the authenticity of the lotus bee pollen, the AF is used as anindicative substance, and when the content of the AF in the lotus bee pollen is 600-850 mg / kg, the AF can be judged as real lotus bee pollen; otherwise, the bee pollen is judged as other types of beepollen or adulterated lotus bee pollen. The method provided by the invention also has the advantages of simplicity, accuracy and the like, is convenient to operate and popularize, and has important practical significance for protecting the legitimate rights and interests of lotus bee pollen consumers and maintaining the healthy development of the bee product consumption industry.

Description

technical field [0001] The invention relates to the field of food testing, in particular to a method for evaluating the authenticity of lotus bee pollen. Background technique [0002] Bee pollen is the pollen collected by bees from plants that are the source of nectar, mixed with their own secretions to form pollen clusters, and stored in the hive. Bee pollen is the food of bees, and it is also the main source of protein for bees. Studies have found that bee pollen is rich in nutrients, such as protein, amino acid, phenolic flavonoids and minerals, so it has good beauty and health effects on the human body, and is deeply loved by young women and the elderly. [0003] At present, the total output of bee pollen in my country is about 10,000 tons, of which more than 5,000 tons can enter the market and become a commodity. Although there are many types of bee pollen plants, the varieties that can produce large quantities of bee pollen mainly include rapeseed powder, camellia po...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 杨术鹏李熠张玉豪周金慧张金震杨宇晖黄京平金玥赵文王鹏
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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