Method for extracting and separating ganoderma triterpene extract by using subcritical technology

A technology of Ganoderma lucidum triterpenes and subcritical water, which is applied in the field of subcritical water extraction and separation of Ganoderma lucidum triterpenoid active extracts, can solve the problem that it is difficult to truly reflect the extraction rate of Ganoderma lucidum triterpenoid components in subcritical extraction, and the unspecified Ganoderma lucidum triterpenoid acids are not specified. Purity and pharmacological activity, unexplained Ganoderma lucidum polysaccharide purity and pharmacological activity, etc., to achieve good antioxidant activity, inhibit tumor cell growth activity, and low cost

Active Publication Date: 2020-06-30
福建仙芝楼生物科技有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Deng Chenchen et al. ("Study on the technology of extracting Ganoderma lucidum polysaccharides with subcritical water", Deng Chenchen et al., Journal of Henan University of Technology (Natural Science Edition), 2016, 37(2): 105-108) although the conditions for extracting Ganoderma lucidum polysaccharides with subcritical water To optimize, but still need to go through the steps of ultrafiltration, protein removal, and alcohol precipitation to obtain Ganoderma lucidum polysaccharides. The steps are cumbersome, and the purity and pharmacological activity of Ganoderma lucidum polysaccharides obtained are not explained.
[0007] Xu Ruiling et al. ("Optimization of subcritical water extraction of ganoderma acid components in ganoderma lucidum fruiting bodies", Xu Ruiling et al., West China Pharmaceutical Journal, 2019, 34(4): 393~395) on subcritical extraction of ganoderma acid (i.e., the acidic part of the triterpenoids of Ganoderma lucidum) components are optimized, but the liquid phase detection conditions use Ganoderma acid A and Ganoderma acid C2 as reference substances, and only detect the total content of Ganoderma acid A and Ganoderma acid C2, because they are in the Ganoderma acid The proportion of the acidic part of Ganoderma lucidum is too small, it is not easy to reflect the complexity of Ganoderma lucidum acid; since the neutral part of Ganoderma lucidum triterpenes is not detected, it is difficult to truly reflect the extraction rate of subcritical extraction of Ganoderma lucidum triterpenoids
At the same time, the purity and pharmacological activity of the ganoderma triterpene acid obtained by it are not stated.

Method used

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  • Method for extracting and separating ganoderma triterpene extract by using subcritical technology
  • Method for extracting and separating ganoderma triterpene extract by using subcritical technology
  • Method for extracting and separating ganoderma triterpene extract by using subcritical technology

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Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0037] 1. Crush the fruiting bodies of Ganoderma lucidum, and take 1kg of Ganoderma lucidum particles of 40-60 mesh;

[0038] 2. Add 10kg of weight water to Ganoderma lucidum granules, extract at a pressure of 5Mpa and a temperature of 170°C for 10min, filter, rinse with 10kg of water, and combine to obtain filtrate I; after concentration, the relative density of filtrate I is 1.00-1.10 at 25°C , add 95% ethanol alcohol precipitation, alcohol precipitation final concentration is 70%, centrifugation, separation factor 6000~8000 during centrifugation, the turbidity of supernatant is below 100NTU, obtains Ganoderma lucidum triterpene acidic part (sample) after supernatant concentration drying 1); the content of triterpenoid acid parts in Ganoderma lucidum reaches 29.2%. After the precipitate was dried, the polysaccharide fraction of Ganoderma lucidum was obtained (sample 5);

[0039] 3. Add 10kg of water to the Ganoderma lucidum particles extracted in step 2, extract for 10min a...

specific Embodiment 2

[0041] 1. Crush the fruiting bodies of Ganoderma lucidum, and take 1kg of Ganoderma lucidum particles of 40-60 mesh;

[0042] 2. Add 10kg of weight water to Ganoderma lucidum granules, extract at a pressure of 5Mpa and a temperature of 180°C for 10min, filter, add 10kg of water to wash, and combine to obtain filtrate Ⅰ; the relative density of filtrate Ⅰ after concentration is 1.00~1.10 (25°C) , add 95% ethanol alcohol precipitation, alcohol precipitation final concentration is 70%, centrifugation, separation factor 6000~8000 during centrifugation, the turbidity of supernatant is below 100NTU, obtains Ganoderma lucidum triterpene acidic part (sample) after supernatant concentration drying 2), the content of the triterpenoid acidic part of Ganoderma lucidum reached 28.2%; the polysaccharide part of Ganoderma lucidum was obtained after precipitation and drying (sample 6);

[0043] 3. Add 10kg of water to the Ganoderma lucidum particles extracted in step 2, extract for 10min at a...

specific Embodiment 3

[0045] 1. Crush the fruiting bodies of Ganoderma lucidum, and take 1kg of Ganoderma lucidum particles of 40-60 mesh;

[0046] 2. Add 10kg weight of water to Ganoderma lucidum granules, extract at a pressure of 5Mpa and a temperature of 190°C for 10min, filter, add 10kg of water to wash, and combine to obtain filtrate Ⅰ; the relative density of filtrate Ⅰ after concentration is 1.00-1.10 (25°C) , add 95% ethanol alcohol precipitation, alcohol precipitation final concentration is 70%, centrifugation, separation factor 6000~8000 during centrifugation, the turbidity of supernatant is below 100NTU, obtains Ganoderma lucidum triterpene acidic part (sample) after supernatant concentration drying 3), the content of the triterpenoid acidic part of Ganoderma lucidum reached 25.5%; the polysaccharide part of Ganoderma lucidum was obtained after precipitation and drying (sample 7);

[0047] 3. Add 10kg of water to the Ganoderma lucidum particles extracted in step 2, extract for 10min at a...

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Abstract

The invention provides a method for extracting and separating ganoderma triterpene extract by using a subcritical technology. The method comprises the following steps of: sequentially extracting and separating active ingredients, namely a ganoderma triterpene acid part and a ganoderma triterpene neutral part, in ganoderma from ganoderma sporocarp by using the subcritical water extraction technology under different pressure and temperature conditions. According to the method, different active extracts in ganoderma are efficiently and economically obtained by using the subcritical water extraction technology. The antioxidant activity of the DPPH clearance rate of the ganoderma triterpene acid part in the obtained active extract can reach 88.38%, the semi-inhibition ratio IC50 of the ganoderma triterpene neutral part to human large cell lung cancer cells H460 is 95.0 micrograms / ml, and the semi-inhibition ratio IC50 of the ganoderma triterpene neutral part to human hepatoma carcinoma cells HepG2 is 77.7 micrograms / ml.

Description

technical field [0001] The invention belongs to the field of natural product extraction and edible and medicinal fungi, relates to the technical field of processing and utilization of Ganoderma lucidum, in particular to a method for subcritical water extraction and separation of active triterpenoid extracts of Ganoderma lucidum Background technique [0002] Ganoderma lucidum (Leyss.ex Fr.) Karst.) is a kind of Ganoderma lucidum in the polyporaceae fungus (Chinese Pharmacopoeia 2015 edition one), sweet in taste, flat in nature, strengthening the body, and the main active ingredients are Ganoderma lucidum polysaccharide and Ganoderma triterpenoids , Ganoderma lucidum triterpenes have anti-oxidation, enhance immunity, anti-tumor, liver protection and other pharmacological effects. Ganoderma lucidum triterpenoids are divided into acidic part and neutral part, and the acidic part contains pentacyclic triterpene acids such as ganoderma acid A which have been reported in a large nu...

Claims

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Application Information

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IPC IPC(8): A61K36/074A61P39/06A61P35/00
CPCA61K36/074A61P39/06A61P35/00A61K2236/331A61K2236/37A61K2236/55A61K2236/53Y02P20/54
Inventor 周岩飞李晔姚渭溪朱忠敏林真陈桂秋吴长辉许建华郑光耀
Owner 福建仙芝楼生物科技有限公司
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