Method for inducing castanea henryi tissue culture seedling leaves to generate adventitious roots

A technology of tissue culture seedlings and leaves, applied in the field of plant tissue culture, can solve the problems of low multiplication coefficient, easy browning, difficult rooting, etc.

Active Publication Date: 2020-07-07
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, most of the reports on the tissue culture of Chinese chestnut are the establishment of the regeneration system of Chinese chestnut seed embryos, the induction of Chinese chestnut callus, and the research on different explant disinfection methods, etc., and the leaves of Chinese chestnut contain tannins, polyphenols and other substances. , there are problems such as easy browning, low multiplication coefficient, and difficult rooting, and there is no report on the induction of adventitious roots in leaves of Chestnut tissue culture seedlings

Method used

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  • Method for inducing castanea henryi tissue culture seedling leaves to generate adventitious roots
  • Method for inducing castanea henryi tissue culture seedling leaves to generate adventitious roots
  • Method for inducing castanea henryi tissue culture seedling leaves to generate adventitious roots

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Embodiment 1

[0038] A method for inducing the direct generation of adventitious roots from leaves of tissue-cultured plantlets of Chestnut, comprising the following steps:

[0039]S1. Cultivation of Chinese chestnut tissue culture seedlings: Peel the seeds of Chinese chestnut, wash them with tap water for 20 minutes, disinfect them with 75% alcohol for 90 seconds on the ultra-clean workbench, rinse them with sterile water for 3 times; then wash them with 0.1% alcohol mercuric chloride solution for 9 minutes, rinse with sterile water 5 to 6 times. On the filter paper after high-temperature sterilization, part of the cotyledons of the seeds were excised with a sterile blade to obtain seed embryos with a size of 1 cm×0.6 cm×0.6 cm. Inoculate into the first MS medium after autoclaving. Placed in a light culture room for 25-35 days. The temperature of the light cultivation room is 27±1°C; the light cycle is 14 / 10h, and the light intensity is 50molm -2 the s -1 . The above mediums all conta...

Embodiment 2

[0051] A method for inducing the direct generation of adventitious roots from leaves of tissue-cultured plantlets of Chestnut, comprising the following steps:

[0052] S1. Cultivation of Chinese chestnut tissue culture seedlings: Peel the seeds of Chinese chestnut, wash them with tap water for 20 minutes, disinfect them with 75% alcohol for 90 seconds on the ultra-clean workbench, rinse them with sterile water for 3 times; then wash them with 0.1% alcohol mercuric chloride solution for 9 minutes, rinse with sterile water 5 to 6 times. On the filter paper after high-temperature sterilization, part of the cotyledons of the seeds were excised with a sterile blade to obtain seed embryos with a size of 1 cm×0.6 cm×0.6 cm. Inoculate into the first MS medium after autoclaving. Placed in a light culture room for 25-35 days. The temperature of the light cultivation room is 27±1°C; the light cycle is 14 / 10h, and the light intensity is 50molm -2 the s -1 . The above mediums all cont...

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Abstract

The invention relates to a method for inducing castanea henryi tissue culture seedling leaves to generate adventitious roots. The method comprises the following steps: S1, taking down fresh leaves ofcastanea henryi tissue culture seedlings and pricking the fresh leaves; S2, transferring the pricked fresh leaves into a second MS culture medium for inoculation, performing dark culture and then performing illumination culture to generate adventitious roots, wherein the second MS culture medium further comprises cane sugar, agar, IBA and zeatin. The method is not influenced by seasons and can beused for annual culture production; and the adventitious roots are directly generated through the leaves, callus induction is not needed, the culture time is shortened, and cost reduction is facilitated.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for inducing adventitious roots from leaves of tissue-cultured seedlings of Chestnut chestnut. Background technique [0002] Castanea henryi is a plant of the genus Chestnut in the family Fagaceae, and is one of the unique woody food tree species in my country, known as the "hardcore crop". Chestnut is mainly distributed in subtropical hilly areas south of the Qinling Mountains in my country, including Zhejiang, Fujian, Hunan, Jiangxi, Sichuan, Guizhou and other places. Chestnut nuts are rich in starch, sweet and delicious, and are an excellent forest health food. Chestnut bark and fungus contain tannin, which can extract tannins; roots can be used as medicine, decoction and washing can cure sores, and adventitious roots can be used as sustainable resources for medicine. The development of chestnut production can not only ensure the food security of our co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/008A01H4/001
Inventor 熊欢毛小雪邹锋袁德义王荣洁唐窈
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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