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Surface enhanced Raman spectroscopy rapid detection method for ciprofloxacin in eggs and milk

A surface-enhanced Raman and ciprofloxacin technology, which is applied in Raman scattering, measuring devices, and material analysis through optical means, can solve problems such as long cycle times, cumbersome processing procedures, and loose drug incompatibility

Inactive Publication Date: 2020-07-07
OPTOTRACE SUZHOU TECH
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Problems solved by technology

Eggs and milk are important sources of protein food in the lives of Chinese residents, so safety issues should not be underestimated. However, in terms of the current domestic breeding situation, there are still widespread phenomena such as excessive use of antibiotics and lax enforcement of drug incompatibility.
[0003] The detection method of ciprofloxacin mentioned in existing studies and reports is mainly high-performance liquid chromatography. Although the detection limit is low, the sensitivity and accuracy are high, the pretreatment process is cumbersome, the cycle is long, and the cost is high. The personnel requirements are high, and the practicability is greatly limited

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  • Surface enhanced Raman spectroscopy rapid detection method for ciprofloxacin in eggs and milk

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Embodiment Construction

[0020] The present invention will be described in further detail below in conjunction with the examples, but the present invention is not limited to the following examples.

[0021] A surface-enhanced Raman spectrum rapid detection method for ciprofloxacin in eggs and milk, specifically comprising the following steps:

[0022] (1) Prepare ciprofloxacin-negative milk samples and prepare ciprofloxacin-positive milk samples at a concentration of 0.1ppm;

[0023] (2) Take 1 mL of the sample to be tested in a 2 mL centrifuge tube, add 300 μL of milk reagent A, shake for 30 s, and centrifuge at 10,000 r / min for 120 s to separate layers;

[0024] (3) Take all the supernatant in a 2 mL centrifuge tube, add 500 μL of ethyl acetate, oscillate to mix, then centrifuge at 10,000 r / min for 3 min to separate the layers, and the supernatant is the solution to be tested;

[0025] (4) Take 500ul enhanced reagent A, 50ul test solution, and 100ul enhanced reagent B in the detection bottle and mi...

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Abstract

The invention discloses a surface enhanced Raman spectroscopy rapid detection method for ciprofloxacin in eggs and milk, and belongs to the technical field of food safety detection; and the method comprises the following steps: removing protein from an egg (egg white) or milk sample to be detected, extracting ciprofloxacin by using ethyl acetate, uniformly mixing the obtained supernatant to be detected with a proper amount of nano enhancement reagent A and enhancement reagent B, and detecting by using a Raman spectrometer. According to the detection method, a pretreatment method is newly built, the ciprofloxacin in the egg or the milk is detected by using a surface enhanced Raman spectrum technology, the detection limit is 0.1 ppm, the sample pretreatment is simple and convenient, the useamount of samples and reagents is small, the detection is rapid, simple and convenient, the precision is high, and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and in particular relates to a surface-enhanced Raman spectrum rapid detection method for ciprofloxacin in eggs and milk. Background technique [0002] Ciprofloxacin is a synthetic third-generation quinolone antibacterial drug with broad-spectrum antibacterial activity and good bactericidal effect. Its antibacterial activity against almost all bacteria is 2-4 times stronger than that of norfloxacin and enoxacin. Enterobacter, Pseudomonas aeruginosa, Haemophilus influenzae, Neisseria gonorrhoeae, Streptococcus, Legionella, and Staphylococcus aureus have antibacterial effects and are one of the commonly used veterinary drugs in animal husbandry. Eggs and milk are an important source of protein food in the lives of Chinese residents, so safety issues should not be underestimated. However, the current domestic farming situation still shows that there are still widespread phenomena such ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 常化仿倪天瑞马宁贾梦伟章祥尧伟峰
Owner OPTOTRACE SUZHOU TECH
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