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Application of fenbendazole for preparing anti-tumor medicine

An anti-tumor drug, the technology of fenbendazole, applied in the field of biomedicine, can solve the problems of poor accuracy, poor overall treatment effect, high toxicity and side effects of recurrence and metastasis anti-tumor treatment, achieve strong synergy and improve anti-cancer effect , the effect of reducing the dosage of drugs

Active Publication Date: 2020-07-10
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The complex pathogenesis of lung cancer, the occurrence of treatment antagonism, the high incidence of recurrence and metastasis, the high toxicity and side effects of anti-tumor treatment, and poor precision are all important reasons for the poor overall treatment effect.

Method used

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  • Application of fenbendazole for preparing anti-tumor medicine
  • Application of fenbendazole for preparing anti-tumor medicine
  • Application of fenbendazole for preparing anti-tumor medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Cell Proliferation and Colony Formation Experiments

[0029] The tumor cell proliferation experiment was carried out by MTT assay, and the effect of fenbendazole on cell proliferation was analyzed. 3000 cells / well were plated in a 96-well plate, and fenbendazole with different drug concentrations (0.05-200 μM) was added after the cells adhered to the wall, and 25 μl / well MTT solution (5 mg / ml) was added after 48 hours of drug action. After continuing to incubate for 4 hours, the culture was terminated, and the culture supernatant in the well was carefully aspirated and discarded. Add 150ul DMSO to each well to dissolve the crystals. Measure the light absorption value of each well at a wavelength of 490nm with an enzyme-linked immunosorbent monitor, and calculate the IC50 value. The results are as follows: figure 1 shown. The results showed that the drug can inhibit the proliferation of lung cancer cell lines in a dose-dependent manner after 48 hours of acti...

Embodiment 2

[0032] The cell cycle is a tightly regulated orderly event in which genomic DNA replicates and the genome divides equally into two similar cells. The cell cycle can be divided into four phases: G1, S, G2 and M phases. When the cell receives a signal that it is not conducive to division, or the time is not ripe, the cell will stop at the check point of the cell cycle and check. When the time is right, continue to split. When the check reveals an irreparable error, the cell initiates apoptosis. Spread the cells evenly in a 6-well plate, add different concentrations of fenbendazole (0.5-2 μM) after the cells adhere to the wall, collect the cells after 24 hours of action, and add pre-cooled 75% ethanol to fix at -20°C. Afterwards, collect the cells by centrifugation, wash the cells once with 1mL of PBS, add 500uL ethidium bromide (PI) and incubate in the dark for 10 minutes, and then run the flow cytometer to detect the cycle distribution of the cells. The results are shown in ...

Embodiment 3

[0034] Lung cancer cells were evenly spread into 6-well plates, and after being treated with different concentrations of fenbendazole (0.5-2 μM) for 48 hours, the fenbendazole-induced lung cancer was detected by the Annexin V-FITC / PI double staining method on a flow cytometer The effect of apoptosis. It was found that fenbendazole can induce apoptosis in PC-9, H460 and A549 cells in a dose-dependent manner after 48 hours of action. The results are shown in Figure 4 . Figure 4 The results showed that fenbendazole can significantly induce apoptosis of lung cancer cells.

[0035] The Hoechst 33258 apoptosis staining kit was used to stain the nucleus, and the degree of nuclear deformation was judged by observing the fluorescent staining of the nucleus, thereby reflecting the situation of cell apoptosis. It was found that after different concentrations of fenbendazole acted on PC-9 and H460 cells for 24 hours, the nucleus of the cells underwent apoptosis such as nuclear pyknosi...

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Abstract

The invention discloses application of fenbendazole for preparing an anti-tumor medicine. An experiment result indicates that the fenbendazole can obviously inhibit the proliferation of lung carcinomacells, in addition, when the fenbendazole and curcumin are jointly utilized, a strong synergistic effect is shown, and therefore, the fenbendazole and the curcumin can be used as a medicine composition to achieve a better anti-tumor effect.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of fenbendazole in the preparation of antitumor drugs. Background technique [0002] Tumor is one of the main causes of human death, and its morbidity and mortality are generally increasing year by year. In my country, with the acceleration of urbanization, the morbidity and mortality of lung cancer rank first among malignant tumors for a long time. Although many advances have been made in lung cancer research, including screening and treatment, the overall survival rate of lung cancer patients is still low, and the tumor recurrence rate remains unchanged. The complex pathogenesis of lung cancer, the occurrence of treatment antagonism, the high incidence of recurrence and metastasis, the side effects of anti-tumor treatment, and the poor precision are all important reasons for the poor overall treatment effect. Therefore, the development of new antitumor d...

Claims

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Application Information

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IPC IPC(8): A61K31/4184A61P35/00A61P11/00A61K31/12
CPCA61K31/12A61K31/4184A61P11/00A61P35/00A61K2300/00
Inventor 赵承光黄晓颖林士崇杨乐和周峰项友群胡万乐周斌朱宇
Owner WENZHOU MEDICAL UNIV
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