Acorus tatarinowii saccharide polymer as well as preparation method and application thereof

A technology of polymers and calamus sugar, which is applied in the field of medicine, can solve the problems of prevention and treatment of neurodegenerative diseases that have not been reported yet

Active Publication Date: 2020-07-10
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main components of Acorus calamus are volatile oils, alkaloids, sugar polymers, flavonoids, etc. Among them, there are many research reports on volatile oils and alkaloids, and their crude sugar polymers, refined sugar polymers and their prevention and treatment of neurodegenerative diseases research has not yet been reported

Method used

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  • Acorus tatarinowii saccharide polymer as well as preparation method and application thereof
  • Acorus tatarinowii saccharide polymer as well as preparation method and application thereof
  • Acorus tatarinowii saccharide polymer as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The extraction method of embodiment 1 calamus glycopolymer

[0057] 1) Material selection: select the dried rhizome of Acorus calamus, cut into small sections, soak in water for 6-12 hours;

[0058] 2) Water extraction: Extract the rhizome of Acorus calamus soaked in step 1) with 10 times the volume of hot water (80° C.) for 4 times, 3 hours each time, collect the extract, and dry the medicinal residues;

[0059] 3) Fractional alcohol precipitation: the extract obtained in step 2) was concentrated under reduced pressure at 60° C., and ethanol was added for alcohol precipitation, so that the volume concentration of ethanol was 50%, and left standing at room temperature for 24 hours, divided into supernatant 1 and precipitation 1, Collect the precipitate 1 to obtain the crude sugar polymer AT1; Concentrate the supernatant 1 again under reduced pressure at 60°C, add ethanol for alcohol precipitation, so that the volume concentration of ethanol is 70%, let it stand at room ...

Embodiment 2

[0062] The extraction and separation method of embodiment 2 Acorus calamus sugar polymer AT1-3

[0063] 1) Material selection: select the dried rhizome of Acorus calamus, cut into small sections, soak in water for 6-12 hours;

[0064] 2) Water extraction: Extract the rhizome of Acorus calamus soaked in step 1) with 10 times the volume of hot water (80° C.) for 4 times, 3 hours each time, collect the extract, and dry the medicinal residues;

[0065] 3) Fractional alcohol precipitation: Concentrate the extract obtained in step 2) at 60°C under reduced pressure, add ethanol to make the volume concentration of ethanol 50%, let stand at room temperature for 24h, divide into supernatant 1 and precipitate 1, collect the precipitate and Raw sugar polymer AT1 was obtained;

[0066] 4) Purification: the crude sugar polymer AT1 was deproteinized by the Sevag method, and after the deproteinization, the crude sugar polymer was dialyzed with a dialysis bag (molecular weight cut-off of 1000...

Embodiment 3

[0105] Example 3 Study on Anti-Alzheimer's Disease Activity of Acorus Calamus Raw Sugar Polymers AT1, AT2, and ATB

[0106] 1. Experimental method

[0107] 1.1 Experimental design and grouping:

[0108] A total of 120 male KM mice aged 6 weeks in SPF grade were randomly divided into 6 groups, 20 in each group. Each group was administered separately: normal group NC: normal saline; model group SCO: normal saline; positive control group HA: huperzine A (2 mg / kg); AT1 group: calamus glycopolymer AT1 (500 mg / kg); AT2 group: Acorus glycopolymer AT2 (500 mg / kg); ATB group: Acorus glycopolymer ATB (500 mg / kg). After intragastric administration for 30 minutes every day, the normal group was intraperitoneally injected with normal saline, and the other groups were intraperitoneally injected with scopolamine (1mg / kg, injection volume: 0.1mL / 10g) to establish models. Weigh once every two days, adjust the dosage according to the change of body weight, and gavage continuously for 14 days...

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Abstract

The invention discloses an acorus tatarinowii saccharide polymer which comprises AT1-3 and/or AT2-2. AT1-3 is a saccharide polymer composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and xylose, and the structural formula of AT1-3 is shown as a formula (I). AT2-2 is a saccharide polymer composed of mannose, rhamnose, glucuronic acid, galacturonic acid,glucose, galactose, arabinose, xylose and fucose, and the structural formula of AT2-2 is shown as a formula (II). AT1-3 and AT2-2 have the activity of preventing and treating neurodegenerative diseases, and are screened acorus tatarinowii glycopolymer parts with high content and strong activity. The invention further provides a preparation method of the AT1-3 and the AT2-2.

Description

technical field [0001] The invention belongs to the technical field of medicine, and more specifically relates to a calamus sugar polymer and a preparation method and application thereof. Background technique [0002] Neurodegenerative disease is a disease caused by chronic progressive degeneration of the central nervous system, characterized by the apoptosis of neurons in the brain and spinal cord, such neuron cells generally do not regenerate, with As time goes by, the condition gradually deteriorates. Common neurodegenerative diseases mainly include Alzheimer's disease (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS), Huntington's disease (Huntington's disease, HD) and so on. According to statistics, nearly 50 million people worldwide are affected by AD, and the number is expected to increase steadily in the next decade. The main manifestation of Alzheimer's disease is acquired cognitive dysfunction syndrome. A large number of studies have shown that...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P25/28A61K36/888
CPCC08B37/006C08B37/0003A61K31/715A61P25/28A61K36/888
Inventor 严春艳钟晶张倩邱娴
Owner GUANGDONG PHARMA UNIV
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