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A kind of Acinetobacter producing surfactant and its application

A technology for producing surfactants and Acinetobacter, which is applied in the field of microorganisms, can solve the problems of environmental secondary pollution, poor biodegradability, and harsh environments, and achieve the effects of enhancing microbial activity, improving environmental adaptability, and promoting COD

Active Publication Date: 2021-06-15
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the disadvantages of physical and chemical treatment methods such as the above-mentioned patents are: it is easy to cause secondary pollution to the environment, and the cost is high
However, there are few reports in the literature that the fracturing flowback fluid can be repaired by microbial treatment. The reason may be that: on the one hand, the composition of the fracturing flowback fluid is complex, and its environment (salinity, pH, etc.) is harsh, which is not suitable for Many microorganisms survive; on the other hand, the pH fracturing flowback fluid contains a large amount of hydrophobic organic matter, which limits the degradation and utilization of microorganisms, resulting in poor biodegradability, and it is impossible to find suitable biochemical treatment for fracturing flowback. ideal microorganisms for drainage

Method used

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  • A kind of Acinetobacter producing surfactant and its application
  • A kind of Acinetobacter producing surfactant and its application
  • A kind of Acinetobacter producing surfactant and its application

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Experimental program
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specific Embodiment

[0036] Performance testing for Acinetobacter Y2

[0037] 1. Oil discharge ring experiment Add 30mL ultrapure water to a clean 10cm plate, add 100μL light crude oil dropwise to the water, the crude oil will spread rapidly on the water surface, and after it is stable, slowly add 10μL dropwise with a pipette gun For the upper layer of fermentation broth, measure the diameter of the oil ring, and add an equal volume of ultrapure water dropwise as a blank control. The result is as figure 1 (b) shown.

[0038] 2. Droplet collapse experiment

[0039] 25 µL of the cell-free supernatant was dropped onto the Parafilm, followed by observation of the shape of the droplet and the spread of the droplet on the surface of the Parafilm. Then add methylene blue (which has no effect on the shape of the droplet) into the water droplet to facilitate taking pictures, measure the diameter of the droplet with a ruler, and add an equal volume of ultrapure water as a blank control. The result is as...

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Abstract

The invention relates to the field of microorganisms, and discloses a surfactant-producing Acinetobacter and its application. The Acinetobacter of the present invention is named Y2, which has been preserved in the China Type Culture Collection Center on July 29, 2019. The preservation number is CCTCC NO:M 2019588, and the microbial classification is named Acinetobacter sp.; The 16S DNA sequence of Y2 is shown in SEQ ID NO.1. Acinetobacter Y2 obtained in the present invention has the functions of producing biosurfactant and degrading hydrocarbons at the same time. The biosurfactant produced by Acinetobacter Y2 of the present invention not only has high activity, but also exhibits very high activity in the range of pH (2-12), temperature (4-100°C) and salinity (0-100g / L). Strong tolerance.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a surfactant-producing Acinetobacter and its application. Background technique [0002] Fracturing flowback fluid is a product produced during oil drilling and fracturing operations. It mainly comes from a large amount of well-washing wastewater produced by active water washing before and after construction, fracturing gel-breaking fluid discharged from the wellbore after construction, and construction remaining fracturing base fluid. The fracturing flowback fluid contains impurities such as iron ions, petroleum, dissolved solids, suspended solids, and formation infiltrated water, which need to be effectively treated before being recycled or discharged. [0003] It can be seen from the above that the fracturing flowback fluid has the characteristics of high COD, high viscosity, and complex composition. Therefore, the treatment of the fracturing flowback fluid in the prior art mainly adop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P21/00C02F3/34C12R1/01
CPCC02F3/34C12N1/20C12P21/00C12N1/205C12R2001/01
Inventor 章春芳黄小敏周航海李平原程萍周少雄卜魁勇赵波吴君魏静
Owner ZHEJIANG UNIV