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Actinomucor elegans GD48, fermentation product thereof and microbial inoculum, and applications of actinomucor elegans GD48, fermentation product thereof and microbial inoculum

A technology of mucor radiata and fermentation products, applied in the field of microbiology, to reduce the occurrence of tomato fusarium wilt

Active Publication Date: 2020-07-28
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research on biocontrol strains mainly focuses on Pseudomonas in bacteria, Bacillus subtilis and Trichoderma in fungi, etc. There are few reports on the biological control of tomato fusarium wilt using Mucor

Method used

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  • Actinomucor elegans GD48, fermentation product thereof and microbial inoculum, and applications of actinomucor elegans GD48, fermentation product thereof and microbial inoculum
  • Actinomucor elegans GD48, fermentation product thereof and microbial inoculum, and applications of actinomucor elegans GD48, fermentation product thereof and microbial inoculum
  • Actinomucor elegans GD48, fermentation product thereof and microbial inoculum, and applications of actinomucor elegans GD48, fermentation product thereof and microbial inoculum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1A

[0034] Example 1 Separation, purification and identification of Actinomucor elegans GD48

[0035] Healthy tomatoes grown outdoors were collected in Zhaoyuan County, Heilongjiang (45°48'26.47”N 125°09’20.75”E) / min shaker for 30 minutes, and then take out the root to obtain the rhizosphere soil suspension. Take 1ml of the suspension for gradient dilution, and finally take 10 -4 The soil suspension was used for the isolation of fungi.

[0036] The specific operation of the plate dilution coating method is to draw 30 μL of soil suspension on the PDA plate, spread it evenly with a spreader, so that the liquid is completely absorbed by the plate, and repeat the same sample three times. After culturing in a constant temperature incubator at 30°C for 1 week, fungal colonies of different shapes were picked for further purification.

[0037] Perform plate confrontation experiments on different morphological strains for re-screening. The operation method is as follows:

[0038] A 4m...

Embodiment 2

[0045] The control effect of embodiment 2 tomato sickle wilt

[0046] In this example, a pot experiment was used to verify the antagonistic effect of the strain Actinomucor elegans GD48 on fusarium wilt of tomato in the greenhouse.

[0047] In this example, the GD48 spore suspension was first prepared, that is, the preserved strain was first inoculated on a solid PDA medium, and after the mycelium was overgrown with the medium, a small amount of mycelia was picked in a conical flask containing a liquid PDB medium, and placed at 37°C. , 150r / min shaking culture for 12-16h, remove the mycelia from the obtained culture solution by filtering to obtain a spore suspension, and then count with a hemocytometer under a microscope to count the concentration of the spore suspension. The spore suspension counting method is to use a hemocytometer under a microscope to first measure the number of spores in each small square, and then convert it into the number of spores per milliliter of ba...

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Abstract

The present invention relates to the field of microbiology and specifically discloses actinomucor elegans GD48, a fermentation product thereof and a microbial inoculum, and applications of the actinomucor elegans GD48, the fermentation product thereof and the microbial inoculum. The actinomucor elegans GD48 contains a nucleotide sequence shown in SEQ ID NO.1. The deposit number is CGMCC No.19367.The actinomucor elegans GD48 has an obvious antagonistic effect on a tomato fusarium blight pathogen fusarium oxysporum f.sp.Lycopersici and can significantly reduce occurrence of tomato fusarium blight. The actinomucor elegans GD48 provides a research basis for prevention and control of important soil-borne disease fusarium blight of tomatoes and provides a new idea for green prevention and control of plant diseases.

Description

technical field [0001] The invention relates to the field of microbiology, in particular to Mucor actinosa GD48 and its fermentation product, bacterial agent and application. Background technique [0002] Tomato is an important vegetable crop worldwide, and it is also one of the main vegetables grown in my country. Tomato Fusarium wilt, caused by the specialized Fusarium oxysporum f.sp. Lycopersici (FOL), is a disease that seriously affects tomato yield. Since the disease has no good disease-resistant varieties at present, and it is transmitted through the soil, the pathogen spreads upward from the root infection through the tomato vascular bundle. Once the disease occurs, it is difficult to control it with chemical pesticides. At the present stage, agriculture generally adopts methods such as soil chemical fumigation and application of antifungal pesticides for this disease, but these methods pollute the environment greatly, and the residual pesticide chemicals seriously e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P3/00C12R1/645
CPCC12N1/14C12R2001/645C12N1/145
Inventor 王金婷周欣蔡磊
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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