Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Hefang crucian Ferritin L gene, recombinant protein, preparation method and application and primers of recombinant protein

A technology of recombinant protein and compound crucian carp, which is applied in the field of genetic engineering research and development, can solve the problems of economic loss of freshwater aquaculture, attack on healthy fish breeding, aquatic animal septicemia, etc., to reduce the occurrence of drug-resistant bacteria, promote production efficiency, The effect of broad application potential

Pending Publication Date: 2020-07-31
HUNAN NORMAL UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to rapid climate change and seriously polluted water quality problems, it has dealt a serious blow to fish healthy farming, and it is also a problem that is difficult to solve
Among them, Aeromonas hydrophila is one of the main pathogenic microorganisms of bacterial diseases in freshwater fish, which can cause sepsis in aquatic animals and often cause serious economic losses to the freshwater aquaculture industry.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hefang crucian Ferritin L gene, recombinant protein, preparation method and application and primers of recombinant protein
  • Hefang crucian Ferritin L gene, recombinant protein, preparation method and application and primers of recombinant protein
  • Hefang crucian Ferritin L gene, recombinant protein, preparation method and application and primers of recombinant protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A Hefang crucian carp Ferritin L gene, the nucleotide sequence of which is shown in SEQ ID NO:1. Obtain the ORF sequence of Hefang crucian carp Ferritin L gene with specific restriction site by PCR method, the specific operation steps are as follows:

[0048] Specific primers were designed according to the conservation of the Ferritin L gene sequence. The first primer was the upstream primer Ferritin L-F1: 5'-CCGGAATTCATGTCTCTAGTCAAGCAGAA-3' (its nucleotide sequence is shown in SEQ ID NO: 3), the second The first primer is the downstream primer Ferritin L-R1: 5'-CCGCTCGAGTTAATGATGATGATGATGATGGAGCGTGTGCTTGTCA-3' (its nucleotide sequence is shown in SEQ ID NO: 4), with M-MLV reverse transcriptase kit (Promega, Madison, WI, USA ) reverse-transcribed Hefang crucian carp liver cDNA as a template, amplified Hefang crucian carp Ferritin L gene with an enzyme cutting site by touchdown PCR (touchdown PCR) technology, and obtained PCR amplification products were separated by elec...

Embodiment 2

[0050] The Hefang crucian carp Ferritin L recombinant protein is obtained from the Hefang crucian carp Ferritin L gene coded in Example 1, and its amino acid sequence is shown in SEQ ID NO:2.

[0051] The preparation method of He Fang crucian carp Ferritin L recombinant protein of the present invention, comprises the steps:

[0052] (1) Obtain the ORF sequence of the Ferritin L gene of Hefang crucian carp with specific restriction sites by PCR method:

[0053] The specific operation method is the same as in Example 1, and the amino acid sequence (as shown in SEQ ID NO: 2) is deduced according to the ORF nucleotide sequence of the Hefang crucian carp Ferritin L gene.

[0054] Using two sequence analysis softwares, ClustalX and GeneDoc, the amino acid sequence of Hefang crucian carp Ferritin L (WR-Ferritin L, shown in SEQ ID NO: 2) was compared with the human Ferritin L amino acid sequence (HsFerritin L), and it was found that the synthetic The amino acid sequence of Ferritin L...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Hefang crucian(WR) Ferritin L gene and a recombinant protein thereof. The nucleotide sequence of the WR Ferritin L gene is shown as SEQ ID NO: 1, and the amino acid sequenceof the recombinant protein is shown as SEQ ID NO: 2. The discovery of the WR ferritin L gene enriches a gene pool of WR and can be applied to preparation of the recombinant protein. Experiments provethat the recombinant protein can inhibit the growth activity of aeromonas hydrophila, and a new practical basis is provided for physiological immune research of the WR. The invention further disclosesa preparation method of the WR ferritin L recombinant protein. The invention discloses application of the WR Ferritin L recombinant protein in the fields of inhibition of growth of aeromonas hydrophila, immune preparations for aquatic animals or feed additives, and discloses primers for amplifying the WR Ferritin L gene.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering research and development, and in particular relates to a combined crucian carp Ferritin L gene, a combined crucian carp Ferritin L recombinant protein, a method for preparing a combined crucian carp Ferritin L recombinant protein, and the application and use of a combined crucian carp Ferritin L recombinant protein. Primers for amplifying the Ferritin L gene of Hefang crucian carp. Background technique [0002] Iron ions are one of the essential trace elements in organisms, which are directly related to individual development, oxidative stress and disease occurrence. In addition, biotic stress (infection by bacteria or viruses) or abiotic stress (heavy metals) will cause an imbalance in the dynamic regulation of iron ions in animals and accumulate in cells, causing a sharp increase in the level of reactive oxygen species, and finally affecting intracellular DNA, protein And macromolec...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/12C07K14/47C12N15/70A23K50/80A23K20/147A61K38/17A61P31/04A61P37/00
CPCA23K20/147A23K50/80A61P31/04A61P37/00C07K14/47C12N15/70
Inventor 罗盛伟刘少军罗紫烨罗凯坤覃钦博张纯陶敏刘庆峰胡方舟王石
Owner HUNAN NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com