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Primer set for triple detection of Neisseria gonorrhoeae, Chlamydia trachomatis and Ureaplasma urealyticum, product and application

A technology for Chlamydia trachomatis and Ureaplasma urealyticum, which is applied to the determination/inspection of microorganisms, microorganisms, recombinant DNA technology, etc., can solve the problems of increasing the cost of detection and cumbersomeness, and achieve fast and intuitive detection results, strong repeatability and specificity. Good results

Inactive Publication Date: 2020-07-31
HANGZHOU BIOER TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most products adopt a single detection method for Neisseria gonorrhoeae (NG), Chlamydia trachomatis (CT) or Ureaplasma urealyticum (UU), which increases the cost of detection and the complexity of operation

Method used

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  • Primer set for triple detection of Neisseria gonorrhoeae, Chlamydia trachomatis and Ureaplasma urealyticum, product and application
  • Primer set for triple detection of Neisseria gonorrhoeae, Chlamydia trachomatis and Ureaplasma urealyticum, product and application
  • Primer set for triple detection of Neisseria gonorrhoeae, Chlamydia trachomatis and Ureaplasma urealyticum, product and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0110] This embodiment provides a triple detection kit for Neisseria gonorrhoeae, Chlamydia trachomatis and Ureaplasma urealyticum, including: PCR reaction solution, primer set mixture, positive control substance and negative control substance.

[0111] Among them, the PCR reaction solution includes 10×PCR Buffer, 25mM MgCl 2 , 25mM dNTPs, 100mM dUTP, Taq enzyme and UDG enzyme.

[0112] Primer set mix includes:

[0113] Component (i): consists of a pair of primers for detecting Chlamydia trachomatis and a probe for detecting Chlamydia trachomatis, wherein the base sequences of the two primers are shown in SEQ ID NO.3 and SEQ ID NO.4 respectively, and the probe The base sequence of the probe is shown in SEQ ID No.8, the 5' end of the probe is marked with a fluorescent reporter group (FAM), and the 3' end is marked with a fluorescent quencher group (BHQ-1); wherein, the Chlamydia trachomatis primer The ratio with the probe is: Chlamydia trachomatis-F: Chlamydia trachomatis-R: ...

Embodiment 2

[0144] Embodiment 2 Positive control substance detection result

[0145] The positive control substance was detected using the kit and detection method provided in Example 1 of the present invention. The positive control substance is a mixed plasmid containing target fragments of target genes amplified by Neisseria gonorrhoeae (NG), Chlamydia trachomatis (CT), and Ureaplasma urealyticum (UU). figure 1 .

Embodiment 3

[0146] Embodiment 3 sensitivity test

[0147] Adopt the kit and detection method that the embodiment of the present invention 1 provides to detect:

[0148] The identified clinical samples of Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), and Ureaplasma urealyticum (UU) were diluted 10 times and 10 times respectively as initial samples. 2 times, 10 3 times, 10 4 Times, the test results show that: the kit has good sensitivity, and presents a gradient change, see the results figure 2 , image 3 , Figure 4 .

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Abstract

The invention provides a primer set for the triple detection of Neisseria gonorrhoeae, Chlamydia trachomatis and Ureaplasma urealyticum, a product and an application, and relates to the technical field of molecular biology. The primer set provided by the invention includes a primer pair for detecting Neisseria gonorrhoeae, a primer pair for detecting Chlamydia trachomatis, and a primer pair for detecting Ureaplasma urealyticum, the simultaneous detection of three pathogens of Neisseria gonorrhoeae (NG), Chlamydia trachomatis (CT), and Ureaplasma urealyticum (UU) in one detection system is realized, and the primer set provided by the invention also has the advantages such as high sensitivity, good specificity, strong reproducibility, and rapid and intuitive detection results.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a triple detection primer set, product and application of Neisseria gonorrhoeae, Chlamydia trachomatis and Ureaplasma urealyticum. Background technique [0002] Neisseria gonorrhoeae is a Gram-negative obligate aerobic diplococcus, the main pathogen causing gonorrhea, characterized by a short incubation period and strong infectivity. If it is not cured in time, serious complications and sequelae may occur. According to clinical manifestations, it can be divided into symptomatic urogenital system infection (commonly manifested as suppurative inflammation), asymptomatic urogenital system infection, infection of eyes, pharynx, skin, rectum, pelvic cavity and other parts, and hematogenous disseminated infection. The common manifestations of men are urethritis, and the complications include epididymitis, prostatitis, seminal vesiculitis, etc. The common manifestations of wom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/04C12N15/11C12R1/01C12R1/35
CPCC12Q1/689
Inventor 王善辰陈芝娟王虹军贺贤汉
Owner HANGZHOU BIOER TECH CO LTD
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