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Hepatic extracellular space information acquisition method

A technology of information collection and liver cells, which is applied in the field of information collection in the extracellular space of liver cells, can solve the problems of inability to study the whole liver comprehensively and have many influencing factors, and achieve the effect of realizing dynamic evaluation and avoiding invasive and invasive examination

Pending Publication Date: 2020-08-04
THE SECOND HOSPITAL OF SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, perfusion imaging studies only a certain level of the liver, and cannot conduct a comprehensive study on the whole liver, and there is no uniform standard for the mathematical model of perfusion imaging quantification, and there are large differences in the results obtained by different perfusion models. There are many factors affected by the patient (respiratory exercise, liver congestion, inflammation, etc.), and CT perfusion has certain radiation, while MRI perfusion scan imaging is slower

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  • Hepatic extracellular space information acquisition method
  • Hepatic extracellular space information acquisition method
  • Hepatic extracellular space information acquisition method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] The method for collecting information on the extracellular spaces of the liver in this embodiment includes the following steps:

[0062] (1) Pretreatment: cleaning the sample, then marking the sample, and standing it for use;

[0063] (2) Initial inspection: Paraffin-embedded and HE-stained the sample obtained from the pretreatment in step (1), wherein the thickness of the section after paraffin-embedded was 1 μm, and the specific operation of HE staining was as follows,

[0064] Select the improved hematoxylin stain solution containing ethanol for staining treatment for 4min,

[0065] Choose pure water to rinse for 2 minutes,

[0066] Select the hydrochloric acid immersion 5min that mass concentration is 1%,

[0067] Choose pure water to rinse again for 2 minutes,

[0068] Choose an improved alkaline solution to immerse for 5 minutes,

[0069] Choose pure water for the third rinse for 6 minutes,

[0070] Choose improved eosin dyeing solution for dyeing treatment f...

Embodiment 2

[0084] This embodiment is improved on the basis of Embodiment 1, the difference is:

[0085] In the above-mentioned method for collecting information on the extracellular space of the liver, the puncture operation described in step (1) is specifically to take the patient in the supine position or the inclined position, and use B-ultrasound to outline the patient's liver on the skin surface , and determine the two points farthest apart, then take the center point along the connection line formed between the two points, and draw a circle with the center point as the center and a distance of 2cm-3cm as the radius, where the arc of the circle Take the three points connected to form a triangle, and then use alcohol cotton balls for disinfection. After disinfection, use a biopsy needle to prick the liver tissue.

[0086] In the above-mentioned method for collecting information on the extracellular space of liver cells, the information card described in step (1) is specifically an Io...

Embodiment 3

[0111] With reference to embodiment 1 and embodiment 2, and introduce respectively normal people (normal group) and suffer from the patient of liver disease (hepatic fibrosis group) to test,

[0112] The results are shown in Table 1:

[0113] Table 1 Comparison of hepatic extracellular space between normal group and liver fibrosis group

[0114] group Number of cases fECS liver fibrosis group 32 35.7％±6.8％ normal group 32 26.5％±6.2％

[0115] get conclusion:

[0116] Sensitivity: 100%,

[0117] Specificity: 75%,

[0118] False Positive Rate: 25%,

[0119] False Negative Rate: 0%,

[0120] Youden Index: 0.75

[0121] Positive likelihood ratio: 4.0,

[0122] Negative Likelihood Ratio: 0.0,

[0123] Positive predictive value: 80%,

[0124] Negative predictive value: 100%.

[0125] In addition, in the above experiments, the slice pictures of liver fibrosis group and normal group are as follows: figure 1 shown.

[0126] Summary discussion: ...

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Abstract

The invention discloses a hepatic extracellular space information acquisition method, and belongs to the technical field of data information processing. The method comprises the following steps: (1),performing pretreatment; cleaning a sample, marking the sample, and standing for later use; (2), performing primary detection; to be specific, carrying out paraffin embedding and HE dyeing on the sample obtained by pretreatment in the step (1); and (3), performing final inspection; to be specific, carrying out image scanning on the sample obtained by initial inspection in the step (2), and evaluating the extracellular space of the hepatic cell. According to the method, invasive examination is avoided, fibrosis evaluation and treatment response observation can be repeatedly carried out, dynamicevaluation of pathological hepatic cells can be achieved, and the method has a positive effect on understanding of the dynamic process of liver fibrosis and fibrinolysis.

Description

technical field [0001] The invention belongs to the technical field of data information processing, and in particular relates to a method for collecting extrahepatic space information. Background technique [0002] The concept of hepatic fibroproliferative disease was proposed by the famous hepatologist Hans Popper in 1984. It is now known that liver fibrosis is a group of clinicopathological syndromes. Excessive extracellular matrix hyperplasia and deposition, the pathological change is the excessive deposition of collagen, resulting in decreased microcirculation perfusion under pressure of blood vessels, further development can cause portal hypertension and fatty degeneration and necrosis of liver cells, and finally become irreversible sclerosis. Therefore, hepatic fibrosis is a common and basic link for all types of liver parenchymal damage to cirrhosis. With the in-depth study of the mechanism of liver fibrosis, some scholars have confirmed that some liver fibrosis or e...

Claims

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Application Information

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IPC IPC(8): G01N21/84G01N1/28G01N1/30G01N1/36G01N1/34A61B5/00
CPCA61B5/4244A61B5/4842A61B5/4848G01N1/28G01N1/30G01N1/34G01N1/36G01N21/84
Inventor 李玉亮王维陈超于哲王武杰
Owner THE SECOND HOSPITAL OF SHANDONG UNIV
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