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ZIF-8 nanoparticles coated with anti-aging drug and application of ZIF-8 nanoparticles

A technology of ZIF-8 and nanoparticles, applied in the field of biomedicine, can solve the problems of poor controlled release effect, easy oxidation and inactivation of anti-aging drugs, etc., and achieve the effect of enhancing stability

Inactive Publication Date: 2020-08-07
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Despite the advantages of providing biocompatibility and the ability to absorb many drugs, organic carriers are often poorly controlled release
[0005] In summary, there are generally insurmountable technical problems in the existing technology: how to ensure the easy oxidative inactivation of anti-cell senescence drugs in the carrier system applicable to anti-cell senescence drugs, and how to ensure the anti-cell senescence drug carrier system The problem of the stability of the drug performance of the drug

Method used

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  • ZIF-8 nanoparticles coated with anti-aging drug and application of ZIF-8 nanoparticles
  • ZIF-8 nanoparticles coated with anti-aging drug and application of ZIF-8 nanoparticles
  • ZIF-8 nanoparticles coated with anti-aging drug and application of ZIF-8 nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1. A preparation method of a drug-carrying system for delaying the aging of mesenchymal stem cells

[0038] This example mainly describes a method for preparing a drug-carrying system for delaying the aging of mesenchymal stem cells, including the following processes:

[0039] Weigh out 150 mg of zinc nitrate hexahydrate solution and 330 mg of 2-methylimidazole and dissolve each in a glass jar with 7.15 ml of methanol. 2-Methylimidazole was added to the methanolic solution of zinc nitrate hexahydrate, and the reaction was stirred for 3 hours. After the reaction, ZIF-8 nanoparticles were collected by centrifugation, washed three times with methanol, and finally stored in methanol solution. Then prepare 50 mM resveratrol methanol solution, and then mix the two evenly with resveratrol methanol solution: ZIF-8 nanoparticle methanol solution at a volume ratio of 16:1. Subsequently, the mixture was stirred for 12 h at room temperature and protected from light, and t...

Embodiment 2

[0040] Example 2. Performance testing of the drug-loading system for delaying the aging of mesenchymal stem cells

[0041] According to the drug-loaded system Resveratrol@ZIF-8 prepared in Example 1, this embodiment has carried out a series of performance testing tests of the drug-loaded system Resveratrol@ZIF-8, including scanning electron microscope detection, X-ray diffractometer analysis, infrared spectroscopy ( IR) detection, etc.

[0042] (1) SEM detection

[0043] Dilute ZIF-8 and Resveratrol@ZIF-8 in methanol solution (promote the particles to disperse sufficiently to the mg level, the final concentration is 3mg / mL), disperse evenly by ultrasonication for 20 minutes, and add the droplets to the cleaned silicon Finally, paste the silicon wafer on the sample holder for scanning electron microscope photography. As shown in Figure 1. Such as figure 1 , (a) is the scanning electron microscope result of the prepared ZIF-8 nanoparticles (that is, the ZIF-8 nanoparticles p...

Embodiment 3

[0050] Example 3, Senescence Detection Test of Mesenchymal Stem Cells

[0051] This example mainly detects the aging process of mesenchymal stem cells. The detection process and results are as follows: normal 5th generation cells (mouse bone marrow mesenchymal stem cells) were subcultured, and the medium was replaced after 9th generation. Serum-reduced medium, cultivated to 20 passages. The cells of the 5th generation and the 20th generation were stained for β-galactosidase (β-gal) senescence respectively. From Figure 5a: normal cells of the 5th generation and Figure 5b: the cells of the 20th generation, we found that compared with the 5th generation cells, the 20th generation After the β-gal senescent staining of the cells, green chromogenic substances appeared, indicating that the cells were senescent.

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PUM

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Abstract

The invention provides ZIF-8 nanoparticles coated with an anti-aging drug and the application of the ZIF-8 nanoparticles, the ZIF-8 nanoparticles are used for coating the anti-aging drug, the anti-aging drug is loaded in the ZIF-8 nanoparticles, the function of inhibiting cell aging is achieved, and the main application scenarios include inhibition of cell aging and the like in the cell in-vitro expansion link. In the invention, the ZIF-8 nanoparticles not only play a role of a carrier, but also play a role in enhancing the stability of the anti-aging drug. Different from medicines in other systems, the ZIF-8 nanoparticles coated with the anti-aging medicines disclosed by the invention have the advantages that the scavenging capacity of the anti-aging medicines on free radicals is fully exerted, so that the anti-cell aging effect is realized.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to ZIF-8 nanoparticles wrapped with anti-aging drugs and an application thereof. Background technique [0002] With the rapid development of life science and medicine, cell therapy has made great progress in many fields such as tumor, inflammation, tissue regeneration, and anti-aging. In the process of cell therapy, in vitro expansion and culture are often required to obtain a large number of cells required for treatment. However, cells cultured in vitro quickly enter a state of replicative senescence. This phenomenon greatly hinders the clinical application of cell therapy. Therefore, how to inhibit cell senescence during in vitro expansion is extremely important for the application and development of cell therapy. [0003] Cellular senescence is a cellular fate that essentially includes irreversible cessation of replication, frequent increases in apoptosis resis...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/22A61K31/05A61K31/436A61K31/192A61P39/06
CPCA61K9/5123A61K31/05A61K31/436A61K31/192A61P39/06
Inventor 王国成卢丹萍
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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