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Graphene quantum dot and gadolinium ion chelate magnetic resonance contrast agent and preparation method thereof

A graphene quantum dot and chelate technology, which is applied in nuclear magnetic resonance/magnetic resonance imaging contrast agents, graphene, general/multifunctional contrast agents, etc., can solve the problem of unclear structure of carbon quantum dots and gadolinium complexes, The effect of weighted angiography is not as good as that of high gadolinium content, so as to achieve the effect of excellent weighted angiography performance, easy surface modification and uniform size

Active Publication Date: 2020-08-07
ANHUI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The disadvantage of this method is that the cost of raw materials is expensive, the structure of the obtained composite of carbon quantum dots and gadolinium is very unclear, and the content of gadolinium and the amount used in imaging are high, which will have a great impact on the organism in the later stage
More importantly, the longitudinal relaxation rate r of the contrast agent 1 should be much smaller than the r of the contrast agent of the present invention 1 , so its T 1 The weighted contrast effect is far inferior to the patented technology of the present invention

Method used

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  • Graphene quantum dot and gadolinium ion chelate magnetic resonance contrast agent and preparation method thereof
  • Graphene quantum dot and gadolinium ion chelate magnetic resonance contrast agent and preparation method thereof
  • Graphene quantum dot and gadolinium ion chelate magnetic resonance contrast agent and preparation method thereof

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Embodiment 1

[0040] A kind of preparation method of graphene quantum dot and gadolinium ion chelate specifically comprises the following steps:

[0041] 1) Graphene oxide was prepared using the Hummers method (this method is cited from the literature: ACS Nano, 2010, 4(8), 4806–4814.);

[0042] 2) Weigh 20 mg of the above-mentioned graphene oxide, dissolve it in 60 g of deionized water, treat it with an ultrasonic power of 500 W, and then add 60 g of hydrogen peroxide to fully dissolve it;

[0043] 3) Add 1000 μL of hydrazine hydrate solution to the above solution, then transfer the above solution to a round bottom flask, and reflux in an oil bath at 100°C for 8 hours;

[0044] 4) After the reflux reaction is completed, the above solution is filtered and purified to remove large impurities, and then dried at high temperature (80°C) to obtain GQDs, which have a two-dimensional regular shape, uniform size, and a lateral size of 3- Between 6nm, thickness between 1-2nm, non-excitation light-d...

Embodiment 2

[0056] A kind of preparation method of graphene quantum dot and gadolinium ion chelate specifically comprises the following steps:

[0057] 1) using the Hummers method to prepare graphene oxide;

[0058] 2) Weigh 60 mg of the above graphene oxide, dissolve it in 60 g of deionized water, treat it with an ultrasonic power of 800 W, and then add 60 g to a concentration of 1 mol L -1 The potassium persulfate solution is fully dissolved;

[0059] 3) Add 800 μL of ammonia solution to the above solution, then transfer the above solution to a round bottom flask, and reflux in an oil bath at 70°C for 12 hours;

[0060] 4) After the reflux reaction is completed, the above solution is subjected to chromatographic purification to remove bulk impurities, and then to freeze-drying to obtain GQDs;

[0061] 5) Prepare the GQDs prepared above to a concentration of 0.2 mg mL -1 solution, then add gadolinium chloride, control gadolinium chloride solution concentration is 0.2mmol L -1 , carry...

Embodiment 3

[0064] A kind of preparation method of graphene quantum dot and gadolinium ion chelate specifically comprises the following steps:

[0065] 1) using the Hummers method to prepare graphene oxide;

[0066] 2) Weigh 60 mg of the above-mentioned graphene oxide, dissolve it in 60 g of deionized water, and process it with an ultrasonic power of 600 W, then add a mixture of 20 g of concentrated sulfuric acid and concentrated nitric acid to fully dissolve;

[0067] 3) Add 400 μL, 1mol L to the above solution -1 Sodium hydroxide solution, then transfer the above solution to a round bottom flask, and reflux in an oil bath at 80°C for 10h;

[0068] 4) After the reflux reaction is completed, the above solution is subjected to suction filtration and purification to remove bulk impurities, and then to vacuum drying to obtain GQDs;

[0069] 5) Prepare the GQDs prepared above to a concentration of 0.05 mg mL -1 solution, then add gadolinium chloride to control its solution concentration to...

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Abstract

The invention discloses a graphene quantum dot and gadolinium ion chelate (Gd-GQDs), and belongs to the field of nano materials. The graphene quantum dot and gadolinium ion chelate is a nano material,and the surface of the graphene quantum dot and gadolinium ion chelate is provided with a hydrophilic group; the preparation method comprises the following steps: preparing graphene oxide by using aHummers method, carrying out heating oxidation and purification to obtain pure graphene quantum dots, and chelating the pure graphene quantum dots with Gd < 3 + > to form stable Gd-coated GQDs. The Gd-coated GQDs prepared by the method are easily dispersed in water, a phosphate buffered solution (PBS), a biological culture medium and other aqueous solution systems; good biocompatibility and low toxicity are realized; in a 1.5 T magnetic resonance test system, the T1 weighted contrast agent shows excellent T1 weighted contrast performance, and the r1 relaxation rate of the T1 weighted contrastagent is as high as 72 mM <-1 > s <-1 > and is 20 times higher than the r1 value of an existing commercial T1 weighted magnetic resonance contrast agent Gd-DTPA.

Description

technical field [0001] The invention relates to a graphene quantum dot and gadolinium ion chelate magnetic resonance contrast agent and a preparation method thereof, belonging to the technical field of new nanometer materials. Background technique [0002] Magnetic resonance imaging (MRI) is one of the most widely used diagnostic tools in clinical applications. MRI has many advantages, such as noninvasiveness, high spatial and temporal resolution, and good soft tissue contrast. Still, the intrinsic signal differences between tissues, especially between diseased and normal tissues, are not that great. Therefore, an MRI contrast agent is usually injected before the scan to improve image quality. According to the principle of action, MRI contrast agents can be divided into longitudinal relaxation contrast agents (T 1 contrast agent) and transverse relaxation contrast agent (T 2 contrast agent). Most commonly used T 1 The contrast agent is a gadolinium-based contrast agent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K49/08
CPCA61K49/0002A61K49/0019A61K49/085C09K11/65C01B32/15C01B32/182B82Y30/00B82Y25/00C09K11/025C01B32/198B82Y5/00B82Y20/00B82Y40/00C01P2004/04C01P2004/64C01P2004/80C01P2006/60C01P2006/82
Inventor 毕红王东
Owner ANHUI UNIVERSITY
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