Anti-duck circovirus single-chain antibody and preparation method and application thereof

A technology of duck circovirus and single-chain antibody, which is applied in the field of bioengineering to achieve good virus recognition specificity and good blocking effect
CN111499734AActive Publication Date: 2020-08-07青岛嘉智生物技术有限公司 +2
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
青岛嘉智生物技术有限公司
Publication Date
2020-08-07

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Abstract

The invention discloses an anti-duck circovirus single-chain antibody and a preparation method and application thereof, and relates to the technical field of bioengineering. The amino acid sequence ofthe anti-duck circovirus single-chain antibody is Seq ID NO.1 or Seq ID NO.3. The invention provides a coding gene of the anti-duck circovirus single-chain antibody. The nucleotide sequence of the coding gene is Seq ID NO.2 or Seq ID NO.4. The anti-duck circovirus single-chain antibody ScFv-DuCV is screened by adopting a phage antibody library technology, antibody structure modification is further carried out on the anti-duck circovirus single-chain antibody ScFv-DuCV so that the anti-duck circovirus single-chain antibody ScFv-mDuCV is obtained, and the anti-duck circovirus single-chain antibody ScFv-DuCV and the anti-duck circovirus single-chain antibody ScFv-mDuCV can be used for duck circovirus detection and diagnosis. The anti-duck circovirus single-chain antibody ScFv-mDuCV and the duck circovirus have good virus recognition specificity, meanwhile, the anti-duck circovirus single-chain antibody ScFv-mDuCV has a good blocking effect on the duck circovirus, and a foundation is laidfor research of novel drugs for treating and inhibiting the duck circovirus.
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Description

technical field

[0001] The invention relates to the technical field of bioengineering, in particular to an anti-duck circovirus single-chain antibody and its preparation method and application. Background technique

[0002] In order to detect duck circovirus disease and prevent and control its harm to duck industry, antibody technology has played an important role in duck industry disease. However, the commonly used monoclonal antibody technology has disadvantages such as easy loss of antibody genes, long production cycle, and difficult modification of antibody genes. People are gradually turning their attention to the preparation of antibodies by genetic engineering methods.

[0003] The principle of the phage antibody library is to link the antibody variable region gene with the capsid protein gene of the filamentous phage, and express it on the surface of the phage, perform multiple rounds of affinity adsorption on the antibody library by the antigen, and screen out the...

Claims

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